LOCUS       sequence-421229-        5058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Tong Q, Dalgin G, Xu H, Ting CN, Leiden JM, Hotamisligil GS
  TITLE     Function of GATA transcription factors in preadipocyte-adipocyte 
            transition.
  JOURNAL   Science 2000 Oct 6;290(5489):134-8.
  PUBMED    11021798
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2000-10-6"; volume: "290(5489)"; pages: "134-8"
COMMENT     Sequence Label: pcDNA-GATA3
FEATURES             Location/Qualifiers
     source          1..5058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     123..142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     376..393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    510..531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(546..551,552..569,570..576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    584..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     589..611
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     608..624
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     608..624
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        642..660
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     642..659
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2840..2859)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2841..2859)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2866..2883)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2866..2882)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2875..2897)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3023..3478
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3110..3129)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3320..3341
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3556..3660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3661..3729,3730..4521)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3879..3898)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4692..5058,1..222)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
       61 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      421 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      481 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      541 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      601 tttcacacag gaaacagcta tgaccatgat tacgccaagc tatttaggtg acactataga
      661 atactcaagc tatgcatcca acgcgttggg agctctccgg atccaagctt atcgatttcg
      721 aacccggggt accgaattcc ttctttcttt tttttttttt taaatgcatc aaacaactga
      781 agggaaacaa aactgacagt gtgcccattt ggacatcaga cttagtggtt tcttctgtat
      841 ctccatactt taatccccct atattcctta aaactcttgg cgtccttcat gcctttctta
      901 cagctatcca ggtacaataa agtcttcctc aaatgtacag tatttcttac aatataagtt
      961 atatgcaatg ttcagcaata tttttttcac agcactagag accctgttaa atagggaata
     1021 tgagtctgaa tggcttattc acaaatggga tccggattca gtggttggaa tgcagacacc
     1081 acctcgagct cctttgaaaa aagaagcaaa cattttgctt tctgccttca aaaacacaga
     1141 tctgtcgctt tcgggcttca tgatactgct cctgcgaaaa acgcaagtag aaggggtcgg
     1201 aggaactctt cgcacacttg gagactcctc acgcatgtgg agcagggctc tgcctctcta
     1261 acccatggcg gtgaccatgc tggaagggtg gtgaggtccg aaggagaggc cggagggcgg
     1321 atgcatgggc gtcggtgtgg tcagcatgtg gctggagtgg ctgaagggag agatgtggct
     1381 cagggatgac atgtgtctgg agagagcggc cgggttgaag gagctgctct tggggaagtc
     1441 ctccagcgcg tcatgcacct ttttgcactt tttcgatttg ctagacatct tccggtttcg
     1501 ggtctggatg ccttctttct tcatagtcag gggtctgtta atattatgaa gcttgtagta
     1561 cagcccacag gcattgcaga ccgggtcccc attagcgttc ctcctccaga gggtggtggt
     1621 ggtggtctga cagttcgcgc aggatgtccc tgctctcctt gctgccgaca gccttcgctt
     1681 gggcttgata aggggccggt tctgcccatt cattttatgg tagagtccgc aggcattgca
     1741 aaggtagtgc ccggtaccat ctcgccgcca cagtggggta gaggttgccc cgcagttcac
     1801 acactccctg ccttctgtgc tggatcgtgc cttgggcctc gacttacatc cgaacccggt
     1861 aggggatcct cccagcaggc tgctgggtgg gaagagtcca gagctgtact cgggcacata
     1921 gggcggatag gtggtaatgg ggtggtgggc tgaggatgag gccccaccca gggtggtcat
     1981 gctgcctcga gagtgagacg tctccagctt catgctatct ggcagctgca cctgatactt
     2041 gaggcactct ttctcatctt gcctggccga cccggcggat cccggggtgg acagcgacgg
     2101 gtctggggag acgtctttcg gcggggtggg cgggaaggtg aagagatgag gactggagtg
     2161 gccggccgcc agagaagagg atgaagccgg agggtaaacg gacagaggcc ccggagagcc
     2221 gtggtggatg gacgtcttgg agaaggggct gaggttccag ggcgaggcgg tgtggtggct
     2281 gctcagggct ttgccgccat ccagccaggg cagagatccg tgcagcagag gcgggcggca
     2341 tacctggctc ccgtggtggg tcggaggata cctctgcacc gtagccctga cggagtttcc
     2401 gtagtaggac gggacgtggt tgccttgacc atcgatgtta aaaagtacgt ccacctcttc
     2461 cgtcagcgga tactgagctt ccatgtacga atggccgagg cccgggtggt gcgtgtctgg
     2521 gtgctgaccg ttgaggaccg cggggtgatg gtggctcacc cagcgcggct ggtccgcagt
     2581 cacctccatg tcctcggctg tgctcgcgcc ctctcgcccg gcccggaacc ctcgcgggta
     2641 gagggagggc tgtcggcctg ggaggtccgc ggactcctgc cgtcggaggc ccaggagagg
     2701 ggtcgtttaa tggtttgttt gcagttaagg gtatagaaaa ggagagcaaa aaggagggtt
     2761 tagggaggaa agaaaaatct ttgcgggata gttggaattc ctcgagtcta gaggagcatg
     2821 cgacgtcggg cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
     2881 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     2941 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3001 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3061 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     3121 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3181 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     3241 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3301 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     3361 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     3421 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     3481 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc aggtggcact
     3541 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3601 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3661 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3721 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3781 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3841 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3901 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3961 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4021 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4081 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4141 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4201 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4261 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4321 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4381 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4441 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4501 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4561 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4621 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4801 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5041 gagcgaacga cctacacc
//
