LOCUS       sequence-421249-        7393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LR destination vector for integration into N2a rtTA3 expressing 
            cells with an FRT site in the Rosa26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Roth JF, Braunschweig U, Wu M, Li JD, Lin ZY, Larsen B, Weatheritt 
            RJ, Gingras AC, Blencowe BJ
  TITLE     Systematic analysis of alternative exon-dependent interactome 
            remodeling reveals multitasking functions of gene regulatory 
            factors.
  JOURNAL   Mol Cell. 2023 Dec 7;83(23):4222-4238.e10. doi: 
            10.1016/j.molcel.2023.10.034.
  PUBMED    38065061
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.10"; journalName: "Mol Cell"; date: 
            "2023-12-7- 7"; volume: "83"; issue: "23"; pages: "4222-4238.e10"
COMMENT     Sequence Label: 5'-BirA*-FLAG minimal CMV pCDNA5
FEATURES             Location/Qualifiers
     source          1..7393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(48..95)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     227..246
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    379..603
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     592..609
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    651..775
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(816..1121)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(918..937)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1463..2122)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2037..2056
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2176..2182,2183..2200,2201..2206))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2231..2355)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2368..2391)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(join(2422..3030,3031..3033,3034..3384))
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(3517..3541)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3563..3581
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3600..3618
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3637..3655
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3674..3692
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3891..3910
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3985..4089
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4090..4158,4159..4950)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4308..4327)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5121..5709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5610..5629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5863..5880
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5997..6018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6033..6038,6039..6056,6057..6063)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6071..6087
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6076..6098
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6095..6111
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6095..6111
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6160..6281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6171..6190)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6225..6244
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(6411..7393,1..40))
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(join(6411..7393,1..40))
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
ORIGIN
        1 gaaacttctc gacagacgtc gcggtgagtt caggcttttt ggccaaggaa gttcctatac
       61 tttctagaga ataggaactt cggaatagga acttctaggt acgtgaacca tcaccctaat
      121 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
      181 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
      241 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
      301 ccgccgcgct taatgcgccg ctacagggcg cgtggggata ccccctagag ccccagctgg
      361 ttctttccgc ctcagaagcc atagagccca ccgcatcccc agcatgcctg ctattgtctt
      421 cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac
      481 tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt
      541 ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca
      601 cagtcgaggc tgatcagcgg gtttaaacgg gccctctaga ctcgagtcac accactttgt
      661 acaagaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
      721 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggtcga
      781 cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta
      841 atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg
      901 gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc
      961 accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact ggccaggggg
     1021 atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga
     1081 taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc
     1141 gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt
     1201 ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac
     1261 cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact
     1321 gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat
     1381 cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt
     1441 ttagtaagcc ggatccagat ctttacgccc cgccctgcca ctcatcgcag tactgttgta
     1501 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     1561 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     1621 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     1681 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     1741 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     1801 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     1861 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     1921 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     1981 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     2041 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     2101 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gacggatcct
     2161 aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2221 atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2281 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2341 tttgtacaaa cttgtgggcg cgcctagttt atcgtcatcg tctttgtagt ctggcgcgcc
     2401 gccgccgggg ccagatcccc ccttctcggc gctccgcagg ctgatctcgc cgcccatcca
     2461 gggcttgatg atgccgtcct gctccagcag cagggcgccc tgcttgtcga tgccccggct
     2521 gatgccgaag atctccttgt cgccgatgat cagcttcacg ggccggttga tgaagttgtc
     2581 cagcttctcc caccggctca ggtagggggc caggccctcc tgctcgaaca gctccagggc
     2641 ggcccgcagc tcccggatca gcatggcggc cagggtgttc cggtccaggt tgatgccggc
     2701 ctcctgcagg gtgatccagc cctggttcac cacgctctcc tccacccgcc gcatggccat
     2761 gttgatgccg gcgccgatca cgatctgggc ggcgtcgccg gtcttgccgg tcagctccac
     2821 caggatgccg gccagcttcc ggtcctgcag gtacaggtcg ttgggccact tcacccgcac
     2881 cttgtcggcg cccagcttcc gcagcacctc ggccatcacg atgccgatca ccaggctcag
     2941 gccgatggcg gcggcggggc cctgctccag ccgccagaac atgctcaggt acaggttggc
     3001 gccgaagggg ctgaaccact tccggccccg gccgccccgg ccggcctgct ggtactcggc
     3061 gatgcaggcg tcgccgctct tcagctcgcc gatccggtcc agcaggtact ggttggtgct
     3121 gtcgatcacg ggcagcacgg ccacgctgcc gccgtccagc tggcccagga tctgcttggc
     3181 gttcagcagc tggatgggct cgggcaggct gtagcccttg ccgggcacgg tgaacacgtc
     3241 cacgccccag tcccgcaggg tctggatgtg cttgttgatg gcggcccggc tcatgcccag
     3301 ggtctcgccc agctgctcgc cgctgtggaa ctcgccgttg gccagcaggg cgatcagctt
     3361 caggggcacg gtgttgtcct tcatggtggc gctagctgga tccgagctcg gtaccaagct
     3421 taagtttaaa cgctagagga tccagcctcc gcggaggctg gatcggtccc ggtgtcttct
     3481 atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaacgagc
     3541 tctgcttata tagggggtac cttctctatc actgataggg agctcgagac gtgtcgactt
     3601 ctctatcact gatagggaga cgttctagac gtaccttctc tatcactgat agggagctcg
     3661 agacgtgtcg acttctctat cactgatagg gagacgtgcg gccagcttgg atccctcgag
     3721 acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt
     3781 cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc tcgcgcacta
     3841 ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg cggcatcaga
     3901 gcagattgta ctgagagtgc accatagggg atcgggagat ctcccgatcc gtcgacgtca
     3961 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4021 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4081 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4141 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4201 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4261 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4321 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4381 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4441 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4501 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4561 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4621 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4681 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4741 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4801 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4861 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4921 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4981 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5041 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5101 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5161 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5221 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5281 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5341 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5401 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5461 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5521 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5581 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5641 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5701 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5761 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5821 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5881 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     5941 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6001 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     6061 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6121 gccaagctct agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg
     6181 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     6241 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc
     6301 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     6361 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtagtacgtg ctattccttt
     6421 gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca
     6481 tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg
     6541 gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca
     6601 accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc
     6661 gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc
     6721 aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc
     6781 tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg
     6841 aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca
     6901 tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac
     6961 acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct
     7021 tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc
     7081 atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac
     7141 gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg
     7201 tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct
     7261 ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg
     7321 aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg
     7381 aacttttcga tca
//
