LOCUS       sequence-421252-        8954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG epitope-tagged dKeNLuc ""Red"" BRET reporter in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Labra B, Parag-Sharma K, Powers JJ, Srivastava S, Walker JR, 
            Kirkland TA, Brennan CK, Prescher JA, Amelio AL
  TITLE     Optimized in vivo multispectral bioluminescent imaging of tumor 
            biology using engineered BRET reporters.
  JOURNAL   iScience. 2024 Aug 8;27(9):110655. doi: 10.1016/j.isci.2024.110655. 
            eCollection 2024 Sep 20.
  PUBMED    39252965
REFERENCE   2  (bases 1 to 8954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-08-8"; volume: "27(9):110655. doi"; pages: " 
            10.1016/j.isci.2024.110655. eCollection 2024 Sep 20"
COMMENT     Sequence Label: pLenti_CMV:Flag-dKeNLuc
FEATURES             Location/Qualifiers
     source          1..8954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     211..228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    345..366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(381..386,387..404,405..411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    419..435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     424..446
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     443..459
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     443..459
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     478..498
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        480..498
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        526..752
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             753..933
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    980..1105
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1598..1831
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2016..2060
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2327..2443
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2466..2769
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2770..2973
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2924..2944
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2970..2994
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3053..3076
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3077..3739
                     /codon_start=1
                     /product="dimeric Keima-Red fluorescent protein"
                     /label=dKeima-Red
                     /translation="VSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTVT
                     KGGPLPFAWDILSPLFQYGSIPFTKYPEDIPDYVKQSFPEGYTWERTMNFEDGAVCTVS
                     NDSSIQGNCFIYNVKISGTNFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALKL
                     EGGGHYLCEFKSTYKAKKPVRMPGYHYIDRKLDVTSHNRDYTSVEQCEIAIARHFLLG"
     CDS             3755..4270
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(4313..4333)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4318..4647
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4498..4633
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4657..5256
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4657..4676)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5153..5173
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5323..5911
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5376..5396)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5794..5805)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5998..6231
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6303..6424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6340..6359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6394..6413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6464..6599
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6619..6638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6620..6638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6648..6665)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6648..6664)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6657..6679)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6806..7261
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6893..6912)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7103..7124
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7287..7391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7392..7460,7461..8252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7610..7629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8423..8954,1..57)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8912..8931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      181 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      241 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      301 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      361 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      421 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
      481 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
      541 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      601 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      661 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      721 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      781 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      841 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      901 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      961 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     1021 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     1081 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     1141 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     1201 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     1261 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1321 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1381 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1441 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1501 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1561 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1621 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1681 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1741 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1801 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1861 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1921 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1981 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     2041 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     2101 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     2161 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     2221 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     2281 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     2341 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2401 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata agcttgggag
     2461 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2521 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2581 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2641 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2701 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2761 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     2821 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     2881 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     2941 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     3001 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagaa tggactacaa
     3061 ggacgacgat gacaaggtga gtgtgatcgc taaacaaatg acctacaagg tttatatgtc
     3121 aggcacggtc aatggacact actttgaggt cgaaggcgat ggaaaaggaa agccttacga
     3181 gggagagcag acagtaaagc tcactgtcac caagggtgga cctctgccat ttgcttggga
     3241 tatattatca ccactgtttc agtacggaag cataccattc accaagtacc ctgaagacat
     3301 ccctgattat gtaaagcagt cattccctga gggatataca tgggagagga ccatgaactt
     3361 tgaagatggt gcagtgtgta ctgtcagcaa tgattccagc atccaaggca actgtttcat
     3421 ctacaatgtc aaaatctctg gtacgaactt tcctcccaat ggacctgtta tgcagaagaa
     3481 gacacagggc tgggaaccca gcactgagcg tctctttgca cgagatggaa tgctgatagg
     3541 aaacgattat atggctctga agttggaagg aggtggtcac tatttgtgtg aatttaaatc
     3601 tacttacaag gcaaagaagc ctgtgaggat gccagggtat cactatattg accgcaaact
     3661 ggatgtaacc agtcacaaca gggattacac atctgttgag cagtgtgaaa tagccattgc
     3721 acgccacttt ttgctcggtg atatctccgg aggtatggtc ttcacactcg aagatttcgt
     3781 tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac agggaggtgt
     3841 gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga ttgtcctgag
     3901 cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg
     3961 cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg atgatcatca
     4021 ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat
     4081 cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt
     4141 aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg
     4201 ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat
     4261 tctggcgtaa ggatccggac tagttaacgc gtcgaccagt ggtcgaccct gtggaatgtg
     4321 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4381 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4441 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4501 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4561 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4621 ttttttggag gcctaggctt ttgcaaaaag cttaccatga ccgagtacaa gcccacggtg
     4681 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     4741 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     4801 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     4861 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     4921 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     4981 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     5041 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     5101 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     5161 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     5221 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
     5281 ccgaccgaaa ggagcgcacg accccatgca tcgccagtcg acaatcaacc tctggattac
     5341 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5401 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5461 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5521 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5581 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5641 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5701 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     5761 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5821 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5881 agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac
     5941 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6001 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6061 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6121 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6181 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     6241 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6301 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6421 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     6481 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6541 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6601 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     6661 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     6721 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     6781 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6841 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6901 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6961 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7021 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7081 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7141 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7201 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7261 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7621 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7921 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8941 tcgggtttcg ccac
//
