LOCUS       sequence-421257-        4476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SC_Oryza sativa codon-optimized LHGR-N(Ligand binding domain and rat
            glucocorticoid receptor (rcoLHGR-N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Lee DY, Acosta-Gamboa L, Saleh L, Pathak S, Swyers N, Morgan A, 
            Meerdink S, Kuzio C, Calderon S, Sheng H, Kenney S, Zare A, Gehan M,
            Nusinow DA
  TITLE     Remote Sensing of Endogenous Pigmentation by Inducible Synthetic 
            Circuits in Grasses.
  JOURNAL   Plant Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480.
  PUBMED    41351300
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480."
COMMENT     Sequence Label: pDL0_029
FEATURES             Location/Qualifiers
     source          1..4476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaaatggca tcagaggcac gtaagactaa gaagaagatc aaaggaattc
     2281 agcaggcaac agcaggagtg agccaagaca cctctgagaa tccgaataag acgatagtgc
     2341 cggccgctct gccccaactg actccgacac ttgtgtcact gctggaggta atagagccgg
     2401 aggtcttgta tgccggctac gattcgtccg tccctgacag tgcctggcgc attatgacca
     2461 cgctcaacat gctcggtggg cggcaagtca ttgcggcggt taagtgggcg aaagcaattc
     2521 ctggtttcag gaatctccat ctcgatgacc aaatgaccct cctccaatac tcctggatgt
     2581 tcctgatggc gtttgccctc ggttggcgca gttaccgcca atcaagcgga aacttgctgt
     2641 gctttgcacc tgacttgatc atcaatgaac agaggatgtc gctgccgtgc atgtacgacc
     2701 agtgcaaaca tatgctcttc gtgtcctccg aattgcagcg ccttcaagtg agctacgaag
     2761 aatacttgtg catgaaaact ctgctcttgt tgagttccgt gcctaaggag ggactcaaga
     2821 gccaagagct gtttgatgaa atcaggatga catacatcaa ggagctcggg aaagcgatcg
     2881 taaagcgcga gggtaactca tcacagaact ggcaacgctt ctaccagctg actaagttgc
     2941 tcgattccat gcatgaggta gtcgaaaacc tcctgaccta ttgcttccaa accttcctcg
     3001 ataagaccat gtctattgag tttcccgaga tgctcgcaga gataatcacg aaccagatcc
     3061 ccaagtacag caacggtaat ataaagaagc ttctcttcca tcagaaatct acctctaaac
     3121 cagtgacctt gtatgacgtg gcggagtacg ccggagtcag tcatcaaaca gtttcaaggg
     3181 tggttaacca ggcgtcccac gtttcggcaa agaccagaga aaaagtggag gcagctatgg
     3241 cggaactgaa ttacattcca aaccgcgtgg cacaacaact ggcgggaaag caaagcctcc
     3301 ttattggggt ggcaacgtcc tccctcgctc tgcatgcccc ttctcagatt gttgctgcca
     3361 taaagagtcg cgcagaccag ctcggtgcaa gtgtggttgt ttccatggtc gaaaggtcgg
     3421 gtgtggaggc gtgtaaagcg gccgtgcaca atttgctcgc ccaacgcgtt tcgggcctca
     3481 taattaatta ccctctggac gatcaggatg cgattgccgt agaagcagct tgtacgaacg
     3541 tcccggcgct cttcttggac gtcagcgacc aaaccccgat taattcaatt atattcagcc
     3601 atgaggacgg gacgcgcctc ggcgtggagc acctcgtagc gctgggacat caacaaatag
     3661 cgcttctggc tggtccgctc tcttctgtct cggctcggct taggcttgct gggtggcata
     3721 agtatttgac acgtaatcag atccaaccaa tcgcggagag ggagggcgac tggagtgcta
     3781 tgtctggatt ccaacagact atgcaaatgc tcaatgaggg aatagtgcct accgccatgc
     3841 tggtggccaa tgatcaaatg gcacttggag cgatgcgcgc gattaccgaa tcagggctta
     3901 gagtcggggc tgacatctct gtcgtggggt atgatgacac tgaggactcc tcgtgttata
     3961 tcccacctct tacgacaata aaacaggact tccgccttct ggggcagacg tcagtcgacc
     4021 gccttcttca actgagccag ggtcaggcgg taaagggcaa ccaactcctg cccgtgtctc
     4081 tggtgaagcg taagactaca agtggctcag aattcgctaa tttcaatcag tccgggaata
     4141 tagcggactc gagcctttcc ttcactttta cgaactcgag taacggccct aacctcatca
     4201 ccacacaaac aaacagccag gccctctcgc agccgattgc gtcctcaaac gtccacgata
     4261 atttcatgaa caatgaaatt accgctagca aaatagatga tgggaacaat tcaaaaccac
     4321 tgtcaccagg gtggacagat cagaccgctt acaacgcttt cggtatcacg acgggcatgt
     4381 ttaataccac taccatggac gacgtctaca attacctgtt tgacgatgag gacacaccac
     4441 ctaatccgaa gaaggagtga gctttgagac cacgaa
//
