LOCUS       sequence-421259-        4369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T_Oryza sativa alpha-tubulin A1 promoter_R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Lee DY, Acosta-Gamboa L, Saleh L, Pathak S, Swyers N, Morgan A, 
            Meerdink S, Kuzio C, Calderon S, Sheng H, Kenney S, Zare A, Gehan M,
            Nusinow DA
  TITLE     Remote Sensing of Endogenous Pigmentation by Inducible Synthetic 
            Circuits in Grasses.
  JOURNAL   Plant Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480.
  PUBMED    41351300
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480."
COMMENT     Sequence Label: pDL0_013
FEATURES             Location/Qualifiers
     source          1..4369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcagctttca gcctgaaagg acaaaataca tgttagcgcc ttagtggtac
     2281 attattattt cagtacagca agataacaca attcaaaaga ctgacccaca ataaataact
     2341 agtcctcaat ttaaaatttg agttcctaaa tagacatcta tgaatatgct gtacatcggc
     2401 actacagaaa atacgattcc caataattga acaattgtac tttatttggt tgttactaca
     2461 acaatggaag atacaagatc gtttcaaaac taccatacat gcgtggagta tttgttccac
     2521 agatctggaa aaaacagatc tgacgggcag tgtcaccaaa cactagacat atgtatttgt
     2581 attaggtggg tgacgtgtac aaacatgact accagatcta gaattagaac gcggcgtgct
     2641 ttggaaatgt taagtagatc caaatacatc ggtaacaaat gaacatattc atatgacata
     2701 gctgtaaaac atcatgatct atcatatcaa ctagagggga tctcggcatg tatcattcta
     2761 ttgcatctag aaccatagca tttccatgtg acaaacaatt ttgaaacata cattgctcag
     2821 atctaccata agaacaccat gttcatgaca gcatcgacca tgatttccac atttaacagc
     2881 atcccgaggt cagatccagt gtttagaact atcatttcag caaaattcac aaaataaatc
     2941 ctccaattcg cctacatata tccattccac gcatcctagg accagatcca ccataccagt
     3001 atacacgaac actaaacatt tcatcagatc cgctaactac gacagagaaa acgcagatcc
     3061 agacaaccag atccaccgac aaataaacac agccccccac atccaacaac cgcgaatcca
     3121 cccagatctg acccagaagc aggcacgaag aacacggggg gtgagggaga tgggacgcgg
     3181 atccgtacct ggatgcaagc ttggcggtaa gggttgatgc tgcgacggcg gcggagaagg
     3241 gagagagggg agaggaggaa aagcgcggag gcgcggagag aggcgagtac gaacacgcct
     3301 ttctctgcgt tgtctcggct tagggtttgc gatccccgca ctccgcccgt tttatagggc
     3361 cagacagcct ggacctctca ggagcggaag cgaagggatg ggggagtttt tcgttttacc
     3421 ccctttgacg tgttcaaaat tcactcgcat ctcacatcgt gcctgcaaag gtcgggtggt
     3481 tggatcatat cgagaatatt taataagtta gggagctttc tgataatatc caccgcaagg
     3541 aggccatttc cgtttgcact cggttgaagt ccatacccgc ctctcgttag tttcactgac
     3601 aggtgggtcc agaggcttcc ttcgtgtgcg gttagcgagt gcacgcgtcg ctccaagaaa
     3661 aagcagtagg tgacctggcc acctcgttgt aggtgcgacc gatgcagacg tcccgcttgc
     3721 cggtgggccc acgttacagc tgggtcccac atatcggtgg gtgcgatgag ttgttcggtg
     3781 cgtaggagta gattggatcc caacgggtgg ttgcgtcgtc ggcggccctg ccgattttat
     3841 tttcgatttt tgaaatgcga gagcgggaga acggcaccgt tggcttggct gtggatgccg
     3901 ttagccgcac cggactattc ggcccagttc cattttggcc caacttgaaa cagcgcggag
     3961 cacaagacgg gggagcccat ttaggcccgt aatctcagcc ctgtagaaag tccgtgtcgt
     4021 tgcgatggac cagaaagccc atgggccttg gaggcgttct ctgctgggaa taaaataagt
     4081 tcacactctg tccctcaact ttacgtcgag tttgtttgac atccctaatg cccagtacca
     4141 gaaatctttt gatacaaata aggtttgata taaagtggca cgtggtgaat ggcatcgttt
     4201 gaaaaatctt cagatcgagc gggctccaca agccgtaagt gcaagtgcca aaatttgtga
     4261 agaaaattac ttccttcatt tcacaatgta agttatttta gcattttcca cattcatatt
     4321 gatatcaata aatttagata gatatatatg tctcgcttga gaccacgaa
//
