LOCUS       sequence-421265-        3965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S_Setaria viridis R1 coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Lee DY, Acosta-Gamboa L, Saleh L, Pathak S, Swyers N, Morgan A, 
            Meerdink S, Kuzio C, Calderon S, Sheng H, Kenney S, Zare A, Gehan M,
            Nusinow DA
  TITLE     Remote Sensing of Endogenous Pigmentation by Inducible Synthetic 
            Circuits in Grasses.
  JOURNAL   Plant Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480.
  PUBMED    41351300
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480."
COMMENT     Sequence Label: pDL0_159
FEATURES             Location/Qualifiers
     source          1..3965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaaatggca ctaccagctt ccccagttca ggaagaactg cagccggaga
     2281 gtcaattgag gaaccatctt gctgcagccg tgaggagcat caactggact tatgccctct
     2341 tctggtccat ttcaagcact cagccaggat tcctaacgtg gacggatggg ttctacaacg
     2401 gcgaggtgaa aacgaggaag atcgccaact ctgcggagct gaccgccgac cagctcgtca
     2461 tgcagcgaag cgaacagctg cgggagctct acgaggccct cctctccggc gagtgcgacc
     2521 gccggacggc gcggccgctc gcctcgctgt cgccggagga cctcggcgac accgagtggt
     2581 actacgtggt ctgcatgacc tacgccttcc ggccaggcca agggttgccg ggaaggagct
     2641 ttgctagcaa cgaacgtgtc tggatgcgga actctcacct cgccgacagc aaagccttcc
     2701 cccgcgcgct cctggcgaag agcgcgtcca ttcagacaat cgtctgcatc ccgttcatga
     2761 gcggcgtgct tgagctgggg acaactgatg cggtggtgga ggatccaagc ttgctcagcc
     2821 gagccactgc atctttctgg gagatgcagt ttccggcatg ctcgcaggag ccgagctcca
     2881 gcccgtcagc aaacgaaacc ggcaagcccg ccgacattat cgtgttcgag gacctcgatc
     2941 acattgccat ggaagcgatg atcgccggcg gacaggagct aggcgaagcg gagagcctgt
     3001 cagatggaac cctcgagcaa atcaccaagg agatcgacga attctacagc ctctgcgagg
     3061 aaatggacgt gcagccactc gaggatacct ggatcatgga cgggtctttc gaagtcccct
     3121 cttcgccgca gccggcgccg gggcctgcta ctactaacgc cgccgccacc tcaagcgctc
     3181 tcgttgacgg ctctcgcgcg acgagcttta cggcctgggc gaggccggag tcggactccg
     3241 atgaagtggc tgtgccggtc gtcgaagagc cacagaagct gctgaagaaa gcggtggccg
     3301 gcggagcttg ggcggcgaac aatggcggcg gcggcatgac gagaatggcc caagaaagtg
     3361 gcgtcaagaa ccacgtcatg tcggagagga agcgccggga aaagctcaac gagatgttcc
     3421 tcgttctcaa gtcattggtt ccctccattc acaaggtgga caaagcatcg atccttgccg
     3481 aaacgatagc gtacctcaag gagcttcaac gaagggtaca agagctagaa tccagcaggg
     3541 aacccataat ctcgcgccca tccgaaacaa cgagggcaac aaggcgccat gacgatgagg
     3601 ccgtcaggaa gaaagtctgt gccgcgggct ccaagaggaa gggctcggag ctcggcggtg
     3661 acgtggagag ggagcaccca cgggccctct ccaaggacgg caccagcaac gtcaccgtca
     3721 ccgtatcgga caaggaggtg ctcgtggagg tgcagtgccg gtgggaagag cttctgatga
     3781 cgcgagtgtt cgacgcaatc aagagcctgc aactggacgt tctctcggtt caggcatcgg
     3841 caccggatgg gttcatgggg ctgaagatac gggctcagtt tgccggctca gctgccgttg
     3901 tgccgtggat gatcagcgag gccctccgta atgctatagg gaagcgagga ggttgagacc
     3961 acgaa
//
