LOCUS       sequence-421293-        3876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C1_Pyrearinus termitilluminans beetle luciferase ('Eluc' version - 
            human-codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Lee DY, Acosta-Gamboa L, Saleh L, Pathak S, Swyers N, Morgan A, 
            Meerdink S, Kuzio C, Calderon S, Sheng H, Kenney S, Zare A, Gehan M,
            Nusinow DA
  TITLE     Remote Sensing of Endogenous Pigmentation by Inducible Synthetic 
            Circuits in Grasses.
  JOURNAL   Plant Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480.
  PUBMED    41351300
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Dec 5. doi: 10.1111/pbi.70480."
COMMENT     Sequence Label: pDL0_198
FEATURES             Location/Qualifiers
     source          1..3876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     RBS             3707..3715
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaaggtgag agagagaaga acgtggtgta cggccccgag cccaagcacc
     2281 ctctgggcaa cttcaccgcc ggcgagatgc tgtacaacgc tctgcacaag cactcccaca
     2341 tcccccaggc catcctggac gtgatgggca acgagtccct ttcctaccag gagttcttcg
     2401 acactactgt gaagctgggc cagagcctcc agaactgtgg ctacaagatg aacgatgtcg
     2461 tgtcgatctg tgcagagaac aacaagagat tcttcatccc catcatctcc gcctggtaca
     2521 tcggcatggt ggtggcccct gtgaacgagg actatatccc agacgagctg tgtaaagtga
     2581 ccggcatctc caagccgatc ctggtcttta ccactaggaa gatcctgcct aaggttttgg
     2641 aggttaaaga cagaaccaac tacataaaga ggatcatcat actggactct gaagagaacc
     2701 tgctgggctg cgagagcctg cacaacttca tgtccaggta ctccgacaac aacctccaaa
     2761 cattcaagcc tctgcactac gaccctgtgg accaggtagc cgccatcctg tgctcctccg
     2821 gcacaaccgg cctgcctaaa ggcgtgatgc agacccacag gaacatctgt gtgagactca
     2881 cacacgcatc tgaccccaga gtgggtacac aactcatccc cggcgtatcc gtgctggcct
     2941 acctgccatt cttccacgcc ttcggcttca gtatcaacct gggctatttc atggtgggcc
     3001 tgagagtggt gatgctccga aggtttaacc aggaggtgtt cctgaaggcc atccaggact
     3061 acgaggtgag gagcgtgatc aacgttccct ccacaatcct gttcctgtcc aagagccctc
     3121 tggtggacaa gtacgaccta tccaccctgg cggagctgtg ctgtggagcc gctcctctgg
     3181 cgaaggaggt ggccgagatc gccgtgaaga ggctgaacct gccagggata cggtgtggct
     3241 acggtctaac agagtctacc tccgccaaca tccatactct gcacaacgag ttcaagtccg
     3301 gctccctggg caaggtgaca ccttacatgg ccgccaagat catcgacagg aacaccggcg
     3361 aggccctggg tccaaaccag gtgggcgagc tgtgcatctg gggacctatg gtaacaaaag
     3421 gctatgtgaa caacccacag gctactaagg aggccatcga cgacgacggc tggctgcact
     3481 ctggcgactt cggctactac gacgaggacg agtatttcta catcgtggac cggtacaagg
     3541 agctgatcaa atacaagggc tatcaggtcg cccctgtgga gctggaggag atcctccttc
     3601 agcacccagg catcagggac gtggccgtcg tgggtatccc tgacatcgag gccggcgagc
     3661 tgccagccgg cttcgtggtg aagcagcccg gcgcccaact caccgctaag gaggtgtacg
     3721 acttcctggc ccagagggtg tctcactcca agtacctgag gggcggcgta aggttcgtgg
     3781 actctatccc caggaacgtg acaggcaaga ttagtcgaaa agagctgagg gaggccctga
     3841 tggagaaggc ttctaagctt ggtgtgagac cacgaa
//
