LOCUS       sequence-421626-        8937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP 
            fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Baldwin ET, van Eeuwen T, Hoyos D, Zalevsky A, Tchesnokov EP, 
            Sanchez R, Miller BD, Di Stefano LH, Ruiz FX, Hancock M, Isik E, 
            Mendez-Dorantes C, Walpole T, Nichols C, Wan P, Riento K, Halls-Kass
            R, Augustin M, Lammens A, Jestel A, Upla P, Xibinaku K, Congreve S, 
            Hennink M, Rogala KB, Schneider AM, Fairman JE, Christensen SM, 
            Desrosiers B, Bisacchi GS, Saunders OL, Hafeez N, Miao W, Kapeller 
            R, Zaller DM, Sali A, Weichenrieder O, Burns KH, Gotte M, Rout MP, 
            Arnold E, Greenbaum BD, Romero DL, LaCava J, Taylor MS
  TITLE     Structures, functions and adaptations of the human LINE-1 ORF2 
            protein.
  JOURNAL   Nature. 2024 Feb;626(7997):194-206. doi: 10.1038/s41586-023-06947-z.
            Epub 2023 Dec 14.
  PUBMED    38096902
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06947-z"; journalName: "Nature"; date: 
            "2024-02"; volume: "626"; issue: "7997"; pages: "194-206"
COMMENT     Sequence Label: pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs...
FEATURES             Location/Qualifiers
     source          1..8937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     107..126
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     210..229
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        210..228
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    229..253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             268..290
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             303..320
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             324..1424
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1398..1421
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1476..1499
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4011..4028
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4081..4099)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4095..4142
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4179..4634
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4266..4285)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4476..4497
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4726..5541)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4726..5541)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5449..5468
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5533..5552
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5663..6251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6152..6171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6405..6422
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6437..6576
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6502..6521)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6662..6684
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6678..6869)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7678..8760)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8722..8741
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8761..8838)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
       61 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
      121 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
      181 agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc
      241 ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatga
      301 aacatcacca tcaccatcac cccatgaaaa tcgaagaagg taaactggta atctggatta
      361 acggcgataa aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg
      421 gaattaaagt caccgttgag catccggata aactggaaga gaaattccca caggttgcgg
      481 caactggcga tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc
      541 aatctggcct gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt
      601 ttacctggga tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag
      661 cgttatcgct gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga
      721 tcccggcgct ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc
      781 aagaaccgta cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg
      841 aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc
      901 tgaccttcct ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca
      961 tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat
     1021 ggtccaacat cgacaccagc aaagtgaatt atggtgtaac ggtactgccg accttcaagg
     1081 gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga
     1141 acaaagagct ggcaaaagag ttcctcgaaa actatctgct gactgatgaa ggtctggaag
     1201 cggttaataa agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg
     1261 cgaaagatcc acgtattgcc gccactatgg aaaacgccca gaaaggtgaa atcatgccga
     1321 acatcccgca gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca
     1381 gcggtcgtca gactgtcgat gaagccctga aagacgcgca gactaattcg agctcgaaca
     1441 acaacaacaa taacaataac aacaacccca tgagcctgga agttctgttt cagggccccg
     1501 ccatggacgg taccggatcc cgcatcaaga acctgaccca gagccgcagc accacctgga
     1561 agctgaacaa cctgctgctg aacgactact gggtgcacaa cgagatgaag gccgagatca
     1621 agatgttctt cgaaaccaac gagaacaagg acaccaccta ccagaacctg tgggacgcct
     1681 tcaaggccgt gtgtcgcggc aagttcatcg ccctgaacgc ctacaagcgc aagcaggagc
     1741 gcagcaagat cgacaccctg accagccagc tgaaggagct ggagaagcag gagcagaccc
     1801 acagcaaggc cagccgccgc caggagatca ccaagatccg cgccgagctg aaggagatcg
     1861 agacccagaa gaccctgcag aagatcaacg agtcgcgaag ctggttcttc gagcgcatca
     1921 acaagatcga ccgccccctg gcccgcctga tcaagaagaa gcgcgagaag aaccagatcg
     1981 acaccatcaa gaacgacaag ggcgacatca ccaccgaccc caccgagatc cagaccacca
     2041 tccgcgagta ctacaagcac ctgtacgcca acaagctgga gaacctggag gagatggaca
     2101 ccttcctgga cacctacacc ctgccccgcc tgaaccagga ggaggtggag agcctgaacc
     2161 gccccatcac cggcagcgag atcgtggcca tcatcaacag cctgcccacc aagaagagcc
     2221 ccggccccga cggcttcacc gccgagttct accagcgcta caaggaggag ctggtgccct
     2281 tcctgctgaa gctgttccaa agcatcgaga aggagggcat cctgcccaac agcttctacg
     2341 aggccagcat catcctgatc cccaagcccg gccgcgacac caccaagaag gagaacttcc
     2401 gccccatcag cctgatgaac atcgacgcca agatcctgaa caagatcctg gccaaccgca
     2461 tccagcagca catcaagaag ctgatccacc acgaccaggt gggcttcatc cccgggatgc
     2521 agggctggtt caacatccgc aagagcatca acgtgatcca gcacatcaac cgcgccaagg
     2581 acaagaacca catgatcatc agcatcgacg ccgagaaggc cttcgacaag atccagcagc
     2641 ccttcatgct gaagaccctg aacaagctgg gcatcgacgg cacctacttc aagatcatcc
     2701 gcgccatcta cgacaagccc accgccaaca tcatcctgaa cggccagaag ctggaggcct
     2761 tccccctgaa gaccggcacg cgtcagggct gccccctgag ccccctgctg ttcaacatcg
     2821 tgctggaggt gctggcccgc gccatccgcc aggagaagga gatcaagggc atccagctgg
     2881 gcaaggagga ggtgaagctg agcctgttcg ccgacgacat gatcgtgtac ctggagaacc
     2941 ccatcgtgag cgcccagaac ctgctgaagc tgatcagcaa cttcagcaag gtgagcggct
     3001 acaagatcaa cgtgcagaag agccaggcct tcctgtacac caacaaccgc cagaccgaga
     3061 gccagatcat gggcgagctg cccttcacca tcgctagcaa gcgcatcaag tacctgggca
     3121 tccagctgac ccgcgacgtg aaggacctgt tcaaggagaa ctacaagccc ctgctgaagg
     3181 agatcaagga ggagaccaac aagtggaaga acatcccctg cagctgggtg ggccgcatca
     3241 acatcgtgaa gatggccatc ctgcccaagg tgatctaccg cttcaacgcc atccccatca
     3301 agctgcccat gaccttcttc accgagctgg agaagaccac cctgaagttc atctggaacc
     3361 agaagcgcgc ccgcatcgcc aagagcatcc tgagccagaa gaacaaggcc ggcggcatca
     3421 ccctgcccga cttcaagctg tactacaagg ccaccgtgac caagaccgcc tggtactggt
     3481 accagaaccg cgatatcgac cagtggaacc gcaccgagcc cagcgagatc atgccccaca
     3541 tctacaacta cctgatcttc gacaagcccg agaagaacaa gcagtggggc aaggacagcc
     3601 tgttcaacaa gtggtgctgg gagaactggc tggccatctg ccgcaagctg aagctggacc
     3661 ccttcctgac cccctacacc aagatcaaca gccgctggat caaggacctg aacgtgaagc
     3721 ccaagaccat caagaccctg gaggagaacc tgggcatcac catccaggac atcggcgtgg
     3781 gcaaggactt catgagcaag acccccaagg ccatggccac caaggacaag atcgacaagt
     3841 gggacctgat caagctgaag agcttctgca ccgccaagga gaccaccatc cgcgtgaacc
     3901 gccagcccac cacctgggag aagatcttcg ccacctacag cagcgacaag ggcctgatca
     3961 gccgcatcta caacgagctg aagcagatct actaagcggc cgcactcgag caccaccacc
     4021 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     4081 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     4141 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
     4201 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4261 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4321 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4381 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4441 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4501 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4561 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4621 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4681 tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga aaaactcatc
     4741 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     4801 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     4861 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     4921 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     4981 tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     5041 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     5101 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     5161 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     5221 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     5281 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     5341 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     5401 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     5461 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt
     5521 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     5581 tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     5821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6361 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6421 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6481 accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     6541 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     6601 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6661 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     6721 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     6781 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     6841 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     6901 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     6961 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     7021 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     7081 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     7141 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
     7201 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
     7261 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
     7321 atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt
     7381 ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca
     7441 agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag
     7501 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
     7561 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
     7621 atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca
     7681 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7741 gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac
     7801 gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac
     7861 gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca
     7921 tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc
     7981 ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc
     8041 agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact
     8101 ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca
     8161 gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg
     8221 ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa
     8281 aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt
     8341 gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc
     8401 actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg
     8461 ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc
     8521 gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga
     8581 ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc
     8641 cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga
     8701 aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac
     8761 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
     8821 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
     8881 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcat
//
