LOCUS       sequence-421629-       12601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN 
            array sequence for DNA barcoding (when used in combination with 
            Cas9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12601)
  AUTHORS   Bowling S, Sritharan D, Osorio FG, Nguyen M, Cheung P, 
            Rodriguez-Fraticelli A, Patel S, Yuan WC, Fujiwara Y, Li BE, Orkin 
            SH, Hormoz S, Camargo FD
  TITLE     An Engineered CRISPR-Cas9 Mouse Line for Simultaneous Readout of 
            Lineage Histories and Gene Expression Profiles in Single Cells.
  JOURNAL   Cell. 2020 Jun 11;181(6):1410-1422.e27. doi: 
            10.1016/j.cell.2020.04.048. Epub 2020 May 14.
  PUBMED    32413320
REFERENCE   2  (bases 1 to 12601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.048"; journalName: "Cell"; date: 
            "2020-06-11- 11"; volume: "181"; issue: "6"; pages: "1410-1422.e27"
COMMENT     Sequence Label: pBS31-cCARLIN
FEATURES             Location/Qualifiers
     source          1..12601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     674..690
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     674..690
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        734..1233
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(769..792)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1166..1185
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    1275..1322
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    join(1355..1420,1421..1458)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     polyA_signal    1627..1761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1881..2015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    join(2027..2045,2046..2068,2069..2087,2088..2110,2111..2129,
                     2130..2152,2153..2171,2172..2194,2195..2213,2214..2236,
                     2237..2255,2256..2278,2279..2297)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_RNA        2588..2663
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        3036..3111
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        3484..3559
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        3932..4007
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        4379..4454
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        4836..4911
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        5284..5359
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        5732..5807
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        6179..6254
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        6627..6702
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        6852..7231
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7233..7510
                     /label=chicken beta-actin promoter
     primer_bind     8451..8473
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      8517..8526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8523..8525,8526..8528,8529..9242)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8523..8525,8526..8528,8529..9239)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8568..8589)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8829..8848)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9176..9197
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9552..9569)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9558..9765
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9797..9835
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9832..9856
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          9954..10526
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     rep_origin      complement(10854..11442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10934..10953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11613..12404,12405..12473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12236..12255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12474..12578)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg
      661 cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattaa ttcgtcgacc
      721 tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
      781 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
      841 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
      901 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
      961 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     1021 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     1081 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     1141 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     1201 ctgttctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga tctcgagcag
     1261 ctgaagctta tggagaagtt actattccga agttcctatt ctctagaaag tataggaact
     1321 tcgatccccc tcgatccccc gggctgcaga tctgtagggc gcagtagtcc agggtttcct
     1381 tgatgatgtc atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact
     1441 cttcgcggtc tttccagtgg ggatcgacgg tatcgataag cttgcagatc tgcgactcta
     1501 gaggatctgc gactctagag gatcataatc agccatacca catttgtaga ggttttactt
     1561 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1621 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1681 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1741 gtatcttatc atgtctggat ctgcgactct agaggatcat aatcagccat accacatttg
     1801 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     1861 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     1921 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1981 ccaaactcat caatgtatct tatcatgtct ggatcgagtt taccactccc tatcagtgat
     2041 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2101 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2161 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     2221 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     2281 cctatcagtg atagagaaaa gtgaaagtcg agctctcgag aggtaccttt cccatgattc
     2341 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
     2401 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     2461 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     2521 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgac tgcacgacag
     2581 tcgacgagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     2641 aaaaagtggc accgagtcgg tgcttttttg ttttagagct agaaatagca agttaaaata
     2701 aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac aaatggctct agaggtaccc
     2761 gttgtacgta cgtactttcc catgattcct tcatatttgc atatacgata caaggctgtt
     2821 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     2881 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     2941 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     3001 aaaggacgaa acaccgacac gactcgcgca tacgagtttt agagctagaa atagcaagtt
     3061 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt
     3121 ttagggctag aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt
     3181 ctgcagacaa atggctctag aggtacccgt tcctgcctgc ctgtttccca tgattccttc
     3241 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     3301 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     3361 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     3421 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgactaca gtcgctacga
     3481 cgagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     3541 agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt aaaataaggc
     3601 tagtccgttt ttagcgcgtg cgccaattct gcagacaaat ggctctagag gtacccgttg
     3661 gtcggtcggt ctttcccatg attccttcat atttgcatat acgatacaag gctgttagag
     3721 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
     3781 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
     3841 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     3901 gacgaaacac cgcgagcgct atgagcgact agttttagag ctagaaatag caagttaaaa
     3961 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgttttag
     4021 agctagaaat agcaagttaa aataaggcta gtccgttttt agcgcgtgcg ccaattctgc
     4081 agacaaatgg ctctaaggta cccgttacaa acaaacaatt tcccatgatt ccttcatatt
     4141 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
     4201 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
     4261 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
     4321 tcttggcttt atatatcttg tggaaaggac gaaacaccga tacgatacgc gcacgctagt
     4381 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
     4441 caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc
     4501 cgtttttagc gcgtgcgcca attctgcaga caaatggctc tagaggtacc cgttagtacg
     4561 tacgtaagct tacgttttcc catgattcct tcatatttgc atatacgata caaggctgtt
     4621 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     4681 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     4741 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     4801 aaaggacgaa acaccgagag cgcgctcgtc gactagtttt agagctagaa atagcaagtt
     4861 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt
     4921 ttagagctag aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt
     4981 ctgcagacaa atggctctag aggtacccgt tgccagccag ccatttccca tgattccttc
     5041 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     5101 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     5161 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     5221 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgcgactg tacgcacacg
     5281 cgagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     5341 agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt aaaataaggc
     5401 tagtccgttt ttagcgcgtg cgccaattct gcagacaaat ggctctagag gtacccgttc
     5461 tttctttctt ttttcccatg attccttcat atttgcatat acgatacaag gctgttagag
     5521 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
     5581 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
     5641 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     5701 gacgaaacac cgatagtatg cgtacacgcg agttttagag ctagaaatag caagttaaaa
     5761 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgttttag
     5821 agctagaaat agcaagttaa aataaggcta gtccgttttt agcgcgtgcg ccaattctgc
     5881 agacaaatgg ctctagaggt acccgttagc tagctagctt ttcccatgat tcttcatatt
     5941 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
     6001 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
     6061 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
     6121 tcttggcttt atatatcttg tggaaaggac gaaacaccga gtcgagacgc tgacgatagt
     6181 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
     6241 caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc
     6301 cgtttttagc gcgtgcgcca attctgcaga caaatggctc tagaggtacc cgttcgagcg
     6361 agcgagtttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     6421 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     6481 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     6541 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     6601 aacaccgata cgtagcacgc agacgagttt tagagctaga aatagcaagt taaaataagg
     6661 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt tttagagcta
     6721 gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat tctgcagaca
     6781 aatggctcta gaggtacccg ttatttaatt aaccgcgatg gggatcctct agaaagtata
     6841 ggaacttcga tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     6901 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     6961 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     7021 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     7081 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     7141 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     7201 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
     7261 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     7321 ttgtgcagcg atgggggcgg gggggggggg ggggsgcgcg ccrggcgggg cggggcgggg
     7381 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     7441 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     7501 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     7561 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     7621 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
     7681 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
     7741 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
     7801 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     7861 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     7921 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     7981 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     8041 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     8101 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     8161 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     8221 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     8281 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     8341 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     8401 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     8461 ctggcgtgtg accggcggct ctagagtcga cctgcaggca tgcaagctta ccggtcgcca
     8521 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     8581 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     8641 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     8701 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     8761 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     8821 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     8881 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     8941 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     9001 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     9061 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     9121 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     9181 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     9241 aagcggccgc cggactgcac gacagtcgac gatggagtcg acacgactcg cgcatacgat
     9301 ggagtcgact acagtcgcta cgacgatgga gtcgcgagcg ctatgagcga ctatggagtc
     9361 gatacgatac gcgcacgcta tggagtcgag agcgcgctcg tcgactatgg agtcgcgact
     9421 gtacgcacac gcgatggagt cgatagtatg cgtacacgcg atggagtcga gtcgagacgc
     9481 tgacgatatg gagtcgatac gtagcacgca gacgatggga gctagaattc taactagagc
     9541 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     9601 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     9661 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     9721 cagcaagggg gaggattggg aagagaatag caggcatgct ggggagcggc ctagccccat
     9781 catggatccg ggtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
     9841 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     9901 ccgggaccga tccagcctcc gcggccccga attcacgcgt tgagaacttc agggtgagtt
     9961 tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg
    10021 gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct
    10081 catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt
    10141 tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta
    10201 aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag
    10261 gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta
    10321 aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg
    10381 gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc tttatggtta caatgatata
    10441 cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg
    10501 ttcatgcctt cttctctttc ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc
    10561 atcattttgg caaaggattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
    10621 gtgtggccaa tgccctggct cacaaatacc actgagatcg ttttccctct gccaaaaatt
    10681 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
    10741 tcattgcaat agtgtgttgg aatttctcga tcgctagcat cctaggcgcg ccacatgtga
    10801 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10861 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10921 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10981 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11041 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11101 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11161 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    11221 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11281 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11341 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11401 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    11461 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    11521 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    11581 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    11641 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    11701 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    11761 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    11821 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    11881 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    11941 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    12001 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    12061 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    12121 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    12181 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    12241 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    12301 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    12361 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    12421 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    12481 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    12541 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    12601 c
//
