LOCUS       sequence-42163-e       10428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 10428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ606 - pDESTR4-R3(ttTi14024, X)
FEATURES             Location/Qualifiers
     source          1..10428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3309..3325
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3309..3325
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    3345..3468
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3509..3814)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3611..3630)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(4156..4815)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4730..4749
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(4869..4875,4876..4893,4894..4899))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(4925..5048)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5056..5073)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5056..5072)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5222..5233)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9861..9880)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9980..10002
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10040..10058)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10126..10230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10231..10299,10300..10428,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccat gcttttacat
     1561 ctccatccta gctcacttgt catactatta ccgttcccat agtaattgta caatgccccc
     1621 gttttatcaa gcatttcttt tttttttttg agaattttta aattaatcaa tatcccgagt
     1681 tcttccacct tttgagcaag tctcgttgag gattgcattg gtcccagcgc taactaggcg
     1741 tcaattcatc cacctctcca ttctctctcc cgttccaact gtgtttttct ttgtcagcgg
     1801 cgaattttcc acaaccaatc gagtcctatt tctctttttt tcccccacct cccaataatt
     1861 ttatttgcat gttttataaa ctcactttgt tcaataattt attgatttta ttttttctat
     1921 gatttgaaac tttacatttt tcctactctt ctatagcaat actttgtcac ttacaataac
     1981 cccccccccc cagtctgcct atatttattt agatgttctt tatggccacc caatgtataa
     2041 actcttttag tgcatttgta ggttctttct tcatcccacc caattccccc tccaccacaa
     2101 atcccagttt tgattatttt aagcccatat ttataagaag taaatatatt tatagatttt
     2161 tcttagatta taagctcttc taggttttcc ctcgttgaga agaatgatct gaaaaagcct
     2221 aaattataga tttttgaaac gtattgtgta gaatttgtat ttattcgtga aagtggaata
     2281 aagttaaaac ggaaaaatgt agtattagtt ttgtgcaaaa cattggccct ataaatgtag
     2341 atgtaggaaa gtctgtttat tcttggaaaa atccctaatt tttgtgaaat tgtttggttt
     2401 gcagcggtcg gaagcaatct tgaaaataat tatcatttgt aatcacattt tggtaattta
     2461 ttacgtttat tttcttcatt gattggtagg tatatgtttt ctttcgaaag atgaacaaat
     2521 gatagtgaat tggtgcacct ataaacggaa aatatacctt aaacaattaa tggttaaaaa
     2581 atccacaatg aagaaaagat attagaaaat tccggttgct aagacattga aaaaaacttg
     2641 aaaaaagact ttgaaaactt gacaaaattt ggcacttttg aaaagaaccc atttaaaact
     2701 taaagcatgt actaaaacga gaactttatt tgaaaattat tatatgtgta ttgctgcgct
     2761 tgtacattta aaaaaaggaa ccttttcaat tgatacttaa aacaaataga agttttttaa
     2821 tccatatcct caagcttcgc agataaaccc tcaatatgcc aaactctaat aagtagcaat
     2881 gtaacatgtt tttggcgctg gtgagtaggt actaaaaatt taataaatgt ttccgattta
     2941 aaattttaga tctcgtcaga atcgttgtca acgcagaact acaattctgg catacttttg
     3001 ttataacgat atcaaatacc aaagaactcg tcgaaattgc atacttgttg atggaaccat
     3061 ccgttcaact gaacgccagc cagtttatta cttaaatgaa aactaatttt tcaattgatg
     3121 acggttagtg actttaggtt tcatttgatc agcgtagttg ctgacactta atggttcggg
     3181 aacataaaag aacatgtcga acacgtacat gacgactgct taaaagttga aaatttccca
     3241 tatccgtttc tcattttatg tagtctcttt cagtgtggct actaacgatt ttgcaatttt
     3301 acattataca ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa
     3361 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     3421 aaaacagact acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag
     3481 actggctgtg tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt
     3541 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg
     3601 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
     3661 ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg gggatcacca
     3721 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
     3781 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcggca
     3841 aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
     3901 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccgaaccg
     3961 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
     4021 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
     4081 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa
     4141 gccggatcct ctagattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     4201 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     4261 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     4321 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     4381 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     4441 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     4501 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     4561 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag
     4621 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     4681 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     4741 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     4801 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat cctaactcaa
     4861 aatccacaca ttatacgagc cggaagcata aagtgtaaag cctgggggtg cctaatgcgg
     4921 ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4981 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa ctttattata
     5041 catagttgat aattcactgg ccgtcgtttt acactttgag ccaatttatc caagtccttg
     5101 taaaaagtat tcgaaaattg ttaacggata aaatgtttat tataatatca aaaacaattt
     5161 gtcagttgac cactttttga tatatttgac agaaaccggg atgaattggc tcaaagttag
     5221 ggcgcccttc tattacaggg tttctgataa caaaccggtt attaactccc aacaagggat
     5281 gatttcaatt catcatgctc aaattgaccc aaattaagtt acatgacaaa ttcatcgccc
     5341 ttttcaactc ttcttggctc atcatctgtt attctgttcc tattctgtct gcaccctata
     5401 cccctttgca tactctctcg tcattccttt cttggatagt gtcttcttct ccccagctct
     5461 gctacttcta tgacttgcgc ccgctgcttt tccgctcgtt cctctcctct ctgacgtctt
     5521 cttctgcttc ttttctcctt cctttttccc ttctcaatct tcctcttttt cccatttctg
     5581 tcaatcattc gaagaagaag aagaagaaga ccctcattca tttcattttt ttttctgtcg
     5641 gtgtgtgctg ctcggttaag agtgagctct ctattccacg tcttcttctt tttcttcttc
     5701 gattcgaatc aatcactcca ccaaaacgca ttcgtttttg ggattcaccc cgcggttcgc
     5761 aacaggtttc tttttcaaat tattagcgtt ataaatagaa aaatggtgga gtttcaataa
     5821 aaatgataat ttacaaagtg tattttgatt acatgtactc aaaaaggttg aaatttttca
     5881 taccagtttt ccggaaatcc atctgatatc attatcgtat ttttcttttt aaaaatgttt
     5941 tcaaaaaaaa acaaaatata gctggttatt tggcaccctc taattaccat tttcctgtca
     6001 caccacactc ttttccttct tccacttctt tccgttttca gccgcttcca accaaaccga
     6061 tatgaaagcc gagcaacaac aatcgattcc acccggctcg gcgaccttcc cgtcgcaggt
     6121 gagactcaga aaactagaga aaccgctcaa ctaactcttg atatccgatt tcattctttt
     6181 ctcttttctt tttgttgaac ttttccatat ttccagatgc cacggccacc accaagcacc
     6241 gaacaaggaa tcacaacgga atcggagctt gcgaagaaag ctcaaatcac tccgaacgac
     6301 gttttagcac ttccgggaat cactcaaggt atcttgctcc ctttggattt cagtttaaaa
     6361 cataaattta ggattcttat gctccccatc tgcgaacatc tataacatcg agttcaccaa
     6421 gttccaaatc cgtgatctgg acactgagca agtgcttttc gagatcgcca aaccggagaa
     6481 cgatcaggag aatgatgagt cgccacagga gtcggcaaga tacgtgcgtt atagatttgc
     6541 tccaaacttt ttgaaactca aaacggtcgg agcaactgtg gaattcaaag taggagacat
     6601 cccaatccat catttccgaa tgatcgaacg tcacttcttc aaagatcgcc ttctgaagtg
     6661 ttttgacttt gaattcggat tctgtattcc gaattcacga aacaactgtg aacatatcta
     6721 tgagttccct caactctctc aacaactcag tgagtcatta ttctaaaaag tacaattcaa
     6781 aagactaatc tctttttcag tggacgacat gatcaacaat ccaaacgaga ctcgttctga
     6841 cagcttctat ttcgtcgata acaaactcgt catgcacaac aaagccgact actcatatga
     6901 tgcataaata tttaatacaa aaatgttctg gataattatt ctgtcgaata gaaaaaaaac
     6961 tccaaatgtg attaaattcc aataattcct gtctagtttg ttccttcccc ttccccttct
     7021 catgttcaat gcattcctaa gcttttcagt tccccccttg tttctatatt ttttcgctgt
     7081 cctgtcacac tcgctaaaaa cactaatcac acggaaatct gttttcaata aaaactccaa
     7141 ctttaactca ttttcaattt caactgaaag attttttcat tagagaatgt ctagaactag
     7201 gcccgggcta cgtaatacga ctcacttaag gccttgacta gaggtttgtt tgtgagtttc
     7261 aggaaacttg tgaattcgtt caaacccttc agaatcagaa taaaaaatgg tgctttgtat
     7321 ttcttaaatt ttaataactt tttcacgagc actacatttt aaaattttgt agaagttgtc
     7381 tgtttgcacc catttaattt caaatgtgtc ataagtcaac aactggcacg ttgagtatac
     7441 atagaaaaga atgattttac cttttaatat tttttttgtt ataaaagctt gcaacatttt
     7501 tattacctct atggataaaa aacgaaggct aggagaaaga atctaaaaac tcggattagt
     7561 ttttggatac aaaacagaag attagaatat ttcccttcac gaattttgag ggtaaaacaa
     7621 ttttaccgaa gttttcaaag gtttttgttc aagattccat tcattttttt tgaaatactt
     7681 catacacgtg gtgtcaggtt gttacggttt gacttacaaa ttttgcccaa aaaatgagac
     7741 gtcagcacgt ttttagccat ttaaatcagt taagaactct gtgtctcaac tcccgcattt
     7801 tttgtagata tttgtagatc aaaccgaaat gagacactct gacaccacga gttcatattg
     7861 tgaaactctc ttgaatactt atcactgtgc caacccatgt cacactttta aatacattga
     7921 acagtataaa aaactcaggt caaaagtaac atttgattga tcccccccat ttccttctga
     7981 actttgcttc tcctgttttt gcgtcgtttc gccatcatca ttaaagtgac tcttggactt
     8041 gagactctag ttgcttatcg attggtgatt gaaaaagtag gagaaacgaa tcttttcatc
     8101 tactcctctt ctgcacccgc cttctcttcc tcttcttcat ccagcagtgc catttctctt
     8161 cggcagcaag aatggtgtgt aaagtgatga cggcgcagtt tcttctggca caggagtagc
     8221 acagcacaac ctcagcagta gttggagcaa cccagcagca gcatattttc gatactcatc
     8281 gagcacttgc cgttgtgttg tgtcatccgc caacaaatca gcaaacaacg ttttttatcc
     8341 cgatcattat tttttttcct cgctctgggt tggacttgtc tatgtttcaa tttttattac
     8401 aaaaataatg gaagaaaaac atttgtttga aaattttaat ccctacttta aagttatatt
     8461 atatatagaa aataagtcta aaaaatgttt tattattttt ttaaatttta tttaaaattt
     8521 tgaaaaaaat ttcgtatggc tgatgtccca gacccatcat tttttgtagt tttctgtttt
     8581 tctgaaaatt taaatgacct tctccggttt ttctaagatg acgtcacatt tcaaaaaccc
     8641 attgaaacta attttcagaa aacggaaaac tcaataaaac taagcgtgaa atggaaaaaa
     8701 aatcatttaa aaagttagaa aaatttaaaa aaaagatttc aattctactc ggccattgcg
     8761 atgaattttt tatcaattaa cgctaaatct gtaaaaatta gattaaaaac caaataataa
     8821 agaaacatac aaggaatttc aattttaaaa attgtgtcat caaacttttt gagaaacttc
     8881 agtattaatt taatttcatg gaatgcggtt ttttctctaa ctttttaaca aaactaggta
     8941 gattaaaaaa tttaaacaag accatatata agctactgtt tcaaatctac ctctgtatgt
     9001 atgtacaaca actccttcaa agtgaacgta tttgcgaaaa atgggctcat cacagaagct
     9061 catcattttt tacaacattg tattataaac ttgccagtgc gtagccagca ttttatttaa
     9121 ctttctatga atttttgata ttttttgaaa aagattttct cacatggttc tcaattgaac
     9181 actaaaacat aatctaaaaa ctacgaattt tgagtttaaa gtttttcagt tttaaaggtg
     9241 gagtagcgcc agtagggatt tagtctaagt acaattataa tgacctaaaa caaccaaata
     9301 tcataacaaa acactccgaa aattttcaga ttttttcata atttcggagc tttggcaaat
     9361 tgccaaattt tgaaaaatat gagcttttga ggaaatccaa agcaatgtcg catgttccga
     9421 cccttacaat gtttcaatac aagtaatcaa aacaaaatta aaatataaaa aatgtagaaa
     9481 aaaatgtttt ttttggtcgt cttccaaaat tatgagtggc aaaaactgag taattgtcat
     9541 tttttgacag gaaataaaaa aaattcaaaa attttttgaa aatttttatt atgatatttg
     9601 aatattttag gaccaaagga gtggttttta acagtttccc cacatgcgct actcaacctt
     9661 taaacttcat cccctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9721 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9781 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     9841 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     9901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     9961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
    10021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
    10081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    10141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    10201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
    10261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
    10321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
    10381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatg
//
