LOCUS       sequence-421630-        9603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HseIF4G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9603)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-EGFP-C1-HseIF4G_O
FEATURES             Location/Qualifiers
     source          1..9603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        95..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        399..602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     553..573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     647..666
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        647..665
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      693..702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(699..701,702..704,705..1415)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(699..701,702..704,705..1415)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(744..765)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1005..1024)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1352..1373
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6425..6546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6462..6481)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6516..6535
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6553..7008)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6690..6711)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6902..6921
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7035..7139
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7141..7498
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7141..7332
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7164..7184)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7349..7484
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7533..8327
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7533..8327
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7587..7606)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8197..8216
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8515..8534)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8559..8606
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8935..9523
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9424..9443
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      601 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
      661 atagggagac ccaagctcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc
      721 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      781 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      841 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      901 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      961 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1021 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1081 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1141 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     1201 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1261 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1321 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1381 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagctatga
     1441 acaaagctcc acagtccaca ggccccccac ccgccccatc ccccggactc ccacagccag
     1501 cgtttccccc ggggcagaca gcgccggtgg tgttcagtac gccacaagcg acacaaatga
     1561 acacgccttc tcagccccgc cagggaggat tcaggtctct gcagcacttc taccctagcc
     1621 gggcccagcc cccgagcagt gcagcctccc gagtgcagag tgcagcccct gcccgccctg
     1681 gcccagctgc ccatgtctac cctgctggat cccaagtaat gatgatccct tcccagatct
     1741 cctacccagc ctcccagggg gcctactaca tccctggaca ggggcgttcc acatacgttg
     1801 tcccgacaca gcagtaccct gtgcagccag gagccccagg cttctatcca ggtgcaagcc
     1861 ctacagaatt tgggacctac gctggcgcct actatccagc ccaaggggtg cagcagtttc
     1921 ccactggcgt ggcccccgcc ccagttttga tgaaccagcc accccagatt gctcccaaga
     1981 gggagcgtaa gacgatccga attcgagatc caaaccaagg aggaaaggat atcacagagg
     2041 agatcatgtc tggggcccgc actgcctcca cacccacccc tccccagacg ggaggcggtc
     2101 tggagcctca agctaatggg gagacgcccc aggttgctgt cattgtccgg ccagatgacc
     2161 ggtcacaggg agcaatcatt gctgaccggc cagggctgcc tggcccagag catagccctt
     2221 cagaatccca gccttcgtcg ccttctccga ccccatcacc atccccagtc ttggaaccgg
     2281 ggtctgagcc taatctcgca gtcctctcta ttcctgggga cactatgaca actatacaaa
     2341 tgtctgtaga agaatcaacc cccatctccc gtgaaactgg ggagccatat cgcctctctc
     2401 cagaacccac tcctctcgcc gaacccatac tggaagtaga agtgacactt agcaaaccgg
     2461 ttccagaatc tgagttttct tccagtcctc tccaggctcc cacccctttg gcatctcaca
     2521 cagtggaaat tcatgagcct aatggcatgg tcccatctga agatctggaa ccagaggtgg
     2581 agtcaagccc agagcttgct cctcccccag cttgcccctc cgaatcccct gtgcccattg
     2641 ctccaactgc ccaacctgag gaactgctca acggagcccc ctcgccacca gctgtggact
     2701 taagcccagt cagtgagcca gaggagcagg ccaaggaggt gacagcatca gtggcgcccc
     2761 ccaccatccc ctctgctact ccagctacgg ctccttcagc tacttcccca gctcaggagg
     2821 aggaaatgga agaagaagaa gaagaggaag aaggagaagc aggagaagca ggagaagctg
     2881 agagtgagaa aggaggagag gaactgctcc ccccagagag tacccctatt ccagccaact
     2941 tgtctcagaa tttggaggca gcagcagcca ctcaagtggc agtatctgtg ccaaagagga
     3001 gacggaaaat taaggagcta aataagaagg aggctgttgg agaccttctg gatgccttca
     3061 aggaggcgaa cccggcagta ccagaggtgg aaaatcagcc tcctgcaggc agcaatccag
     3121 gcccagagtc tgagggcagt ggtgtgcccc cacgtcctga ggaagcagat gagacctggg
     3181 actcaaagga agacaaaatt cacaatgctg agaacatcca gcccggggaa cagaagtatg
     3241 aatataagtc agatcagtgg aagcctctaa acctagagga gaaaaaacgt tacgaccgtg
     3301 agttcctgct tggttttcag ttcatctttg ccagtatgca gaagccagag ggattgccac
     3361 atatcagtga cgtggtgctg gacaaggcca ataaaacacc actgcggcca ctggatccca
     3421 ctagactaca aggcataaat tgtggcccag acttcactcc atcctttgcc aaccttggcc
     3481 ggacaaccct tagcacccgt gggcccccaa ggggtgggcc aggtggggag ctgccccgtg
     3541 ggccggctgg cctgggaccc cggcgctctc agcagggacc ccgaaaagaa ccacgcaaga
     3601 tcattgccac agtgttaatg accgaagata taaaactgaa caaagcagag aaagcctgga
     3661 aacccagcag caagcggacg gcggctgata aggatcgagg ggaagaagat gctgatggca
     3721 gcaaaaccca ggacctattc cgcagggtgc gctccatcct gaataaactg acaccccaga
     3781 tgttccagca gctgatgaag caagtgacgc agctggccat cgacaccgag gaacgcctca
     3841 aaggggtcat tgacctcatt tttgagaagg ccatttcaga gcccaacttc tctgtggcct
     3901 atgccaacat gtgccgctgc ctcatggcgc tgaaagtgcc cactacggaa aagccaacag
     3961 tgactgtgaa cttccgaaag ctgttgttga atcgatgtca gaaggagttt gagaaagaca
     4021 aagatgatga tgaggttttt gagaagaagc aaaaagagat ggatgaagct gctacggcag
     4081 aggaacgagg acgcctgaag gaagagctgg aagaggctcg ggacatagcc cggcggcgct
     4141 ctttagggaa tatcaagttt attggagagt tgttcaaact gaagatgtta acagaggcaa
     4201 taatgcatga ctgtgtggtc aaactgctta agaaccatga tgaagagtcc cttgagtgcc
     4261 tttgtcgtct gctcaccacc attggcaaag acctggactt tgaaaaagcc aagccccgaa
     4321 tggatcagta tttcaaccag atggaaaaaa tcattaaaga aaagaagacg tcatcccgca
     4381 tccgctttat gctgcaggac gtgctggatc tgcgagggag caattgggtg ccacgccgag
     4441 gggatcaggg tcccaagacc attgaccaga tccataagga ggctgagatg gaagaacatc
     4501 gagagcacat caaagtgcag cagctcatgg ccaagggcag tgacaagcgt cggggcggtc
     4561 ctccaggccc tcccatcagc cgtggacttc cccttgtgga tgatggtggc tggaacacag
     4621 ttcccatcag caaaggtagc cgccccattg acacctcacg actcaccaag atcaccaagc
     4681 ctggctccat cgattctaac aaccagctct ttgcacctgg agggcgactg agctggggca
     4741 agggcagcag cggaggctca ggagccaagc cctcagacgc agcatcagaa gctgctcgcc
     4801 cagctactag tactttgaat cgcttctcag cccttcaaca agcggtaccc acagaaagca
     4861 cagataatag acgtgtggtg cagaggagta gcttgagccg agaacgaggc gagaaagctg
     4921 gagaccgagg agaccgccta gagcggagtg aacggggagg ggaccgtggg gaccggcttg
     4981 atcgtgcgcg gacacctgct accaagcgga gcttcagcaa ggaagtggag gagcggagta
     5041 gagaacggcc ctcccagcct gaggggctgc gcaaggcagc tagcctcacg gaggatcggg
     5101 accgtgggcg ggatgccgtg aagcgagaag ctgccctacc cccagtgagc cccctgaagg
     5161 cggctctctc tgaggaggag ttagagaaga aatccaaggc tatcattgag gaatatctcc
     5221 atctcaatga catgaaagag gcagtccagt gcgtgcagga gctggcctca ccctccttgc
     5281 tcttcatctt tgtacggcat ggtgtcgagt ctacgctgga gcgcagtgcc attgctcgtg
     5341 agcatatggg gcagctgctg caccagctgc tctgtgctgg gcatctgtct actgctcagt
     5401 actaccaagg gttgtatgaa atcttggaat tggctgagga catggaaatt gacatccccc
     5461 acgtgtggct ctacctagcg gaactggtaa cacccattct gcaggaaggt ggggtgccca
     5521 tgggggagct gttcagggag attacaaagc ctctgagacc gttgggcaaa gctgcttccc
     5581 tgttgctgga gatcctgggc ctcctgtgca aaagcatggg tcctaaaaag gtggggacgc
     5641 tgtggcgaga agccgggctt agctggaagg aatttctacc tgaaggccag gacattggtg
     5701 cattcgtcgc tgaacagaag gtggagtata ccctgggaga ggagtcggaa gcccctggcc
     5761 agagggcact cccctccgag gagctgaaca ggcagctgga gaagctgctg aaggagggca
     5821 gcagtaacca gcgggtgttc gactggatag aggccaacct gagtgagcag cagatagtat
     5881 ccaacacgtt agttcgagcc ctcatgacgg ctgtctgcta ttctgcaatt atttttgaga
     5941 ctcccctccg agtggacgtt gcagtgctga aagcgcgagc gaagctgctg cagaaatacc
     6001 tgtgtgacga gcagaaggag ctacaggcgc tctacgccct ccaggccctt gtagtgacct
     6061 tagaacagcc tcccaacctg ctgcggatgt tctttgacgc actgtatgac gaggacgtgg
     6121 tgaaggagga tgccttctac agttgggaga gtagcaagga ccccgctgag cagcagggca
     6181 agggtgtggc ccttaaatct gtcacagcct tcttcaagtg gctccgtgaa gcagaggagg
     6241 agtctgacca caactgaaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
     6301 caccggatct agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc
     6361 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     6421 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6481 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6541 atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     6601 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     6661 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     6721 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     6781 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     6841 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     6901 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     6961 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     7021 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7081 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     7141 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7201 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7261 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7321 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7381 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7441 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     7501 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     7561 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     7621 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     7681 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     7741 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     7801 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     7861 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     7921 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7981 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     8041 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     8101 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     8161 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     8221 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     8281 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     8341 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     8401 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     8461 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     8521 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     8581 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     8641 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     8701 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     8761 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     8821 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8881 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8941 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9001 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9061 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9121 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9181 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9241 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9301 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9361 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9421 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9481 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9541 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9601 ccc
//
