LOCUS       sequence-421634-        1609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off 
            expression of jRGECO1a (jRGECO1a will not be expressed in mammalian 
            cells that express Cre recombinase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Meng C, Zhou J, Papaneri A, Peddada T, Xu K, Cui G
  TITLE     Spectrally Resolved Fiber Photometry for Multi-component Analysis of
            Brain Circuits.
  JOURNAL   Neuron. 2018 May 16;98(4):707-717.e4. doi: 
            10.1016/j.neuron.2018.04.012. Epub 2018 May 3.
  PUBMED    29731250
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.04.012"; journalName: "Neuron"; date: 
            "2018-05-16- 16"; volume: "98"; issue: "4"; pages: "707-717.e4"
COMMENT     Sequence Label: pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE
FEATURES             Location/Qualifiers
     source          1..1609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             148..165
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             169..201
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(205..213,214..228)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             256..312
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     1008..1027
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgtcgtgag gtaccggatc ctctagagtc gactccggaa caacttcgta tatacctttc
       61 tatacgaagt tgtggcgcgc catgctgcag aacgagcttg ctcttaagtt ggctggactt
      121 gatattaaca agactggagg aggttctcat catcatcatc atcatggtat ggctagcatg
      181 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga tctcgcaaca
      241 atggtcgact catcacgtcg taagtggaat aaggcaggtc acgcagtcag agctataggt
      301 cggctgagct cacccgtggt ttccgagcgg atgtaccccg aggacggcgc cctgaagagc
      361 gagatcaaga aggggctgag gctgaaggac ggcggccact acgccgccga ggtcaagacc
      421 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtgga catcaagttg
      481 gacatcgtgt cccacaacga ggactacacc atcgtggaac agtgcgaacg cgccgagggc
      541 cgccactcca ccggcggcat ggacgagctg tacaagggag gtacaggcgg gagtctggtg
      601 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      661 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      721 tacgaggcct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
      781 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacattaa gcacccagcc
      841 gacatccccg actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg
      901 aacttcgagg acggcggcat tattcacgtg aaccaggact cctccctgca ggacggcgta
      961 ttcatctaca aggtgaagct gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     1021 aagaagacca tgggctggga ggctacgcgt gacgacctga ctgaagagca gatcgcagaa
     1081 tttaaagagg ctttctccct atttgacaag gacggggatg ggacgataac aaccaaggag
     1141 cttgggacgg tgttccgatc gctggggcag aaccccacag aagcagagct gcaggacatg
     1201 atcaatgaag tagatgccga cggtgacggc acattcgact tccctgagtt cctgacgatg
     1261 atggcaagaa aaatgaatga cacagacagt gaagaggaaa ttcgcgaagc gttccgcgtg
     1321 tttgataagg acggcaatgg ctacatcggc gcagcagagc ttcgccacgt gatgacagac
     1381 cttggagaga agttaacaga tgaggaggtt gatgaaatga tcagggtagc agacatcgat
     1441 ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gtagggctag
     1501 cacaacttcg tatatacctt tctatacgaa gttgtgaatt cgatatcaag cttatcgata
     1561 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaa
//
