LOCUS       sequence-421635-        5539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5539)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-HA-SBP_J
FEATURES             Location/Qualifiers
     source          1..5539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        74..2583
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2614..2640
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2662..2775
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             2863..2904
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2914..2931
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2954..2971)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3004..3138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3028..3047)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3082..3101
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3739..4327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3819..3838)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4498..5289,5290..5358))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5121..5140
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5359..5463)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(5531..5539,1..10)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atcacgaggc cctttcgtct tcaagaatta attctatatt ctaaaaacac aaatgatact
       61 tctaaaaaaa aatcatgaat ggcatcaact ctgaatcaaa tctttgcaga tgcacctact
      121 tctcatttcc actgtcacat catttttcca gatctcgctg cctgttatgt ggcccacaaa
      181 ccaagacacg ttttatggcc attaaagctg gctgatcgtc gccaaacacc aaatacataa
      241 tgaatatgta cacattcgag aaagaagcga tcaaagaagc gtcttcgggc ggagtaggag
      301 aatgcggagg agaaggagaa cgagctgatc tagtatctct ccacaatcca atgccaactg
      361 accaactggc catattcgga gcaatttgaa gccaatttcc atcgcctggc gatcgctcca
      421 ttcttggcta tatgtttttc accgttaccc ggggccattt tcaaagactc gtcggcaaga
      481 taagattgtg tcactcgctg tctctcttca tttgtcgaag aatgctgagg aatttcgcga
      541 tgacgtcggc gagtattttg aagaatgaga ataatttgta tttatacgaa aatcagttag
      601 tggaattttc tacaaaaaca tgttatctat agataatttt gttgcaaaat atgttgacta
      661 tgacaaagat tgtatgtata tacctttaat gtattctcat tttcttatgt atttataatg
      721 gcaatgatga tactgatgat attttaagat gatgccagac caaaaggctt gaatttctgc
      781 gtcttttgcc gaacgcagtg catgtgcaat tgttgttttt tggaatattc aattttcgga
      841 ctgtccgctt tgatttcagt ttcttggctt attcaaaaag caaagtaaag ccaaaaaagc
      901 gagatggcaa taccaaatgc ggcaaaacgg tagtggaagg aaaggggtgc ggggcagcgg
      961 aaggaagggt ggggcggggc gtggcggggt ctgtggctgg gcgcgacgtc accgacgttg
     1021 gagccactcc tttgaccatg tgtgcgtgtg tgtattattc gtgtctcgcc actcgccggt
     1081 tgtttttttc tttttatgct gcgctctctc tagcgccatc tcgcttacgc atgctcaacg
     1141 caccgcatgt tgccgtttcc ttttatgcgt cattttggct cgaaataggc aattatttaa
     1201 acaaagatta gtcaacgaaa acgctaaaat aaataagtct acaatatggt tacttattgc
     1261 catgtgtgtg cagccaacga tagcaacaaa agcaacaaca caggtggctt tccctctttc
     1321 actttttgtt tgcaagccgc gtgcgagcaa gacggcacga ccggcaaacg caattacgct
     1381 gacaaagagc agacgaagtt ttggcgaaaa acatcaaggc gcctgatacg aatgcatttg
     1441 caataacaat tgcgatattt aatattgttt atgaagctgt ttgacttcaa aacacacaaa
     1501 aaaaaaaata aaacaaatta tttgaaagag aattaggaat cggacgctta tcgttagggt
     1561 aacaacaaga aatgcttact gagtcacagc ctctggaaaa ctgccgcaag ccagagagag
     1621 agagaaaaag agggagagca gcttagaccg catgtgcttg tgtgtgaggc gtctctctct
     1681 tcgtctctgt tgcgcaaacg catagactgc actgaaaaaa tcgattacct attttttatg
     1741 aatgaatatt tgcactatta ctattcaaaa ctattaagat agcaatcaca ttcaatagcc
     1801 aaatactata ccacctgagc gatgcaacga aatgatcaat ttgagcaaaa atgctgcata
     1861 tttaggacgg catcattata gaaatgcttc ttgctgtgta cttttctctc gtctggcagc
     1921 tgtttcgccg ttattgttaa aaccggctta agttaggtgt gttttctacg actagtgaat
     1981 gccctactag aagatgtgtg ttgcacaaaa tgtccctgga ataaccaatt tgaagtgcag
     2041 atagcagtaa acgtaagcta atatgaatat tatttaactg taatgtttta atatcgctgg
     2101 acattactaa taaacccact ataaacacat gtacatatgt atgttttggc atacaatgag
     2161 tagttgggga aaaaatgtgt aaaagcaccg tgaccatcac agcataaaga taaccagctg
     2221 aagtatcgaa tatgagtaac ccccaaattg aatcacatgc cgcaactgat aggacccatg
     2281 gaagtacact cttcatggcg atatacaaga cacacacaag cacgaacacc cagttgcgga
     2341 ggaaattctc cgtaaatgaa aacccaatcg gcgaacaatt catacccata tatggtaaaa
     2401 gttttgaacg cgacttgaga gcggagagca ttgcggctga taaggtttta gcgctaagcg
     2461 ggctttataa aacgggctgc gggaccagtt ttcatatcac taccgtttga gttcttgtgc
     2521 tgtgtggata ctcctcccga cacaaagccg ctccatcagc cagcagtcgt ctaatccaga
     2581 gaccccggat cggggtacct ggcggccacc atgtacccat acgatgttcc agattacgct
     2641 cccggaagcg gtagcggtac catggacgag aagaccaccg gctggcgggg cggccacgtg
     2701 gtggagggcc tggccggcga gctggagcag ctgcgggcca ggctggagca ccaccctcag
     2761 ggccagcggg agccctccgg cggctgcaag cccccaagct tgaattctgc agatatccag
     2821 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
     2881 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa
     2941 acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc
     3001 gaggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     3061 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3121 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     3181 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     3241 gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     3301 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     3361 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat
     3421 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac
     3481 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga
     3541 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat
     3601 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
     3661 ggtagatgac gaccatcagg gacagcttca aggccagcaa aaggccagga accgtaaaaa
     3721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3901 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4141 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4381 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4441 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4501 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4561 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4621 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4681 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4741 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4801 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4861 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4921 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4981 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5041 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5101 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5161 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5221 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5281 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5341 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5401 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5461 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     5521 aacctataaa aataggcgt
//
