LOCUS       sequence-421639-       10242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-Reporter Plasmid for mRNA 5'UTR Massively Parallel Reporter 
            Assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10242)
  TITLE     A systematic search for RNA structural switches across the human 
            transcriptome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMK089
FEATURES             Location/Qualifiers
     source          1..10242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2069..2090)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2281..2300
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2514..2536
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2528..2545
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2529..2545
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2555..2574
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2555..2573
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2594..2729
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2769..2890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             2962..3195
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3282..3870
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3388..3399
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3797..3817
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3890..4489)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3973..3993)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4470..4489
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4631..5141)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4694..4712)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        5170..5208
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(5257..5259,5260..5262,5263..5976)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5257..5259,5260..5262,5263..5973)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5302..5323)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5563..5582)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5910..5931
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      6410..6419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6416..7126
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6561..6579)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6829..6848
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7322..7456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    7593..7710
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7977..8021)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8206..8439
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8932..9057
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9104..9284
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9299..9502)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        9503..9882
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(9983..10003)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9983..10001)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10022..10038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10022..10038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10035..10057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10046..10062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10070..10076,10077..10094,10095..10100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10115..10136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     2521 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
     2581 gggtacgtcc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     2641 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     2701 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggat agctagagcc
     2761 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2821 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2881 taaacaagtt cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa
     2941 taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     3001 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     3061 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     3121 tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt cgttgggagt
     3181 gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct
     3241 tgtaagtcat tggtcttaaa ggtaccgagc tcgaattcca ggcggggagg cggcccaaag
     3301 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
     3361 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
     3421 cccgcgcaga atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc
     3481 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
     3541 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
     3601 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
     3661 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
     3721 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
     3781 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
     3841 ttcacaaatt ttgtaatcca gaggttgatt gtcgactcta gactcgactt caggcaccgg
     3901 gcttgcgggt catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg
     3961 tgaagccgag ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg
     4021 gcaccccggc gcgctcggcc gcctccactc cggggagcac gacggcgctg cccagaccct
     4081 tgccctggtg gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc tccttgggcc
     4141 ggtgcggcgc caggaggcct tccatctgtt gctgcgcggc cagccgggaa ccgctcaact
     4201 cggccatgcg cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg
     4261 tccagaccgc caccgcggcg ccgtcgtccg cgacccacac cttgccgatg tcgagcccga
     4321 cgcgcgtgag gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccgggtcga
     4381 cggtgtggcg cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggcccggg
     4441 ggacgtcgtc gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatc gcccgcctcc
     4501 cgcggcagcg ctcgacttcc agctcggtcc gctttgcgga ctgatggggc tgcgctgcgc
     4561 tgcgctccag cgccccccct gcccgccgga gctggccgcg gcccgaattc ctgcagcccg
     4621 ggggatcccc ctggggagag aggtcggtga ttcggtcaac gagggagccg actgccgacg
     4681 tgcgctccgg aggcttgcag aatgcggaac accgcgcggg caggaacagg gcccacacta
     4741 ccgccccaca ccccgcctcc cgcaccgccc cttcccggcc gctgctctcg gcgcgccccg
     4801 ctgagcagcc gctattggcc acagcccatc gcggtcggcg cgctgccatt gctccctggc
     4861 gctgtccgtc tgcgagggta ctagtgagac gtgcggcttc cgtttgtcac gtccggcacg
     4921 ccgcgaaccg caaggaacct tcccgactta ggggcggagc aggaagcgtc gccggggggc
     4981 ccacaagggt agcggcgaag atccgggtga cgctgcgaac ggacgtgaag aatgtgcgag
     5041 acccagggtc ggcgccgctg cgtttcccgg aaccacgccc agagcagccg cgtccctgcg
     5101 caaacccagg gctgccttgg aaaaggcgca accccaaccc cgtgggaatt cgatatcaag
     5161 cttctcgagg gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
     5221 cgtcagacgc aggtgaatcg tcagccacct gcacatatgg tgagcaaggg cgaggagctg
     5281 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     5341 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     5401 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     5461 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5521 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     5581 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5641 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     5701 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     5761 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     5821 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     5881 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     5941 gggatcactc tcggcatgga cgagctgtac aagtagacgc gttgtacgta gccagccccc
     6001 gattgggggc gacactccac catagatcac tcccctgtga ggaactactg tcttcacgca
     6061 gaaagcgtct agccatggcg ttagtatgag agtcgtgcag cctccaggac cccccctccc
     6121 gggagagcca tagtggtctg cggaaccggt gagtacaccg gaattgccag gacgaccggg
     6181 tcctttcttg gatcaacccg ctcaatgcct ggagatttgg gcgtgccccc gcaagactgc
     6241 tagccgagta gtgttgggtc gcgaaaggcc ttgtggtact gcctgatagg gtgcttgcga
     6301 gtgccccggg aggtctcgta gaccgtgcac catgagcacg aatcctaaac ctcaaagaaa
     6361 aaccaaacgt aacaccaacc gccgcccaca ggacgtcttc atatgtctag ccaccatggt
     6421 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     6481 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     6541 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     6601 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     6661 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     6721 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     6781 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     6841 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     6901 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     6961 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     7021 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     7081 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtagcggc cggccgcgtc
     7141 gacctagttc taccggtcct gcagggctaa gctggacagc caatgacggg taagagagtg
     7201 acatttttca ctaacctaag acaggagggc cgtcagagct actgcctaat ccaaagacgg
     7261 gtaaaagtga taaaaatgta tcactccaac ctaagacagg cgcagcttcc gagggatttg
     7321 agatccagac atgataagat acattgatga gtttggacaa accaaaacta gaatgcagtg
     7381 aaaaaaatgc cttatttgtg aaatttgtga tgctattgcc ttatttgtaa ccattataag
     7441 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     7501 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     7561 tcagatctct cgaggctagt ctcgtgatcg ataaaatttt gaatttttgt aatttgtttt
     7621 tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg
     7681 taccccccaa tccccccttt tcttttaaaa gttaaccgat accgtcgaga tccgttcact
     7741 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
     7801 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     7861 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     7921 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     7981 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     8041 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     8101 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     8161 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     8221 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     8281 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     8341 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     8401 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     8461 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     8521 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
     8581 aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
     8641 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     8701 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     8761 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
     8821 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     8881 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     8941 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     9001 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     9061 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     9121 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     9181 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     9241 ccagagagct cccaggctca gatctggtct aaccagagag accccggttc actaaacgag
     9301 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     9361 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     9421 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     9481 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     9541 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     9601 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     9661 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     9721 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     9781 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     9841 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacatggc ggtaatgttg
     9901 gacatgagcc aatataaatg tacatattat gatatggata caacgtatgc aatggccaag
     9961 cttgcagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
    10021 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    10081 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    10141 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    10201 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ct
//
