LOCUS       sequence-42164-e        9837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ210 - pDESTR4-R3(ttTi4348, I)
FEATURES             Location/Qualifiers
     source          1..9837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(705..1214)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(900..921)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1108..1127
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      1325..1913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1814..1833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1974..1995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2010..2015,2016..2033,2034..2040)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2048..2064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2053..2075
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2072..2088
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2072..2088
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3701..3717
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3725..3848
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3874..3879,3880..3897,3898..3904)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3958..4617
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4024..4043)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4959..5264
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5143..5162
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5305..5428)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7494..7505
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9298..9314)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9307..9329)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        9535..9639
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9640..9708,9709..9837,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaccccg gttgataatc
      721 agaaaagccc caaaaacagg aagattgtat aagcaaatat ttaaattgta aacgttaata
      781 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
      841 aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagggttg agtgttgttc
      901 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
      961 ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt
     1021 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1081 ggggaaagcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     1141 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     1201 gctacagggc gcgtaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1441 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtaatgtg
     1981 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     2041 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgggtcacca
     2101 ggtatcgcgc tttaattagg gtgcaagttt tttataattt ttaatcccat taaaattcaa
     2161 agcagaaagg ttctgatgct ctacgcaaaa tatcaaaatc acaatttatg acaatattgt
     2221 tacaaaaatc caaaaaaaaa attgcagttc gaaatggtga ttgaagaaaa cggattacgt
     2281 gaagctactc gacgaatcat atcctatttg gaaaaatata ttcatgcact gcactatcat
     2341 cataaggatg atgagattta ataatttttg taatttatct tacaattagc attttattta
     2401 attttaaaaa aatttgaatg ggaacataaa gtcaaattcc tgagctgcat ggctaagggt
     2461 gccctcgaaa attttccgat tgttcgattt atttcagaga aattcggaaa atgcgaaatc
     2521 gaaaaaaagt gtagaaaatc aaaaaatgca aaaatttata ccaaaccgta aaaaattacc
     2581 aaaatttaaa gatattttac ggaaacttac gcttgagtta ttcaaacaca taactgatat
     2641 tataacatta atactatatt aatactagat taatatgtac tatagcaatt ttcaaaatat
     2701 tttcaattag caaaatgttg ccgatcattt ttctacattt acaattaaaa ttaaaatttg
     2761 ataaccctga tccggaaaaa gttacggcag ctgacatttg tctgattttt gactagaatc
     2821 gctaagtggc ccacaatttt taaactaggt gttttcacga aacaaagttt tctatttttt
     2881 tgcaattttt tatcctgtca caaaaggacc ataaaaattt tatacaaaag attaaacatg
     2941 aaactcaaat ttcaaaaatt agagtgtttc gtaaaaagta acgtacatat ggtgccaaaa
     3001 tggccaaata tcatagtaaa atttttcaaa acatttttga aaaaagtggc aattactgag
     3061 tttttgccac ttttttttag taaactatag ctctctcgca tactcgaatt cctacaaaaa
     3121 tttatatttg aaatataagt accataaaat ccggagaaaa acttccaaaa aaatggcaaa
     3181 aactaacatt cttccaattt tcaaaaaatc atataaaatt tggaattttt taaagaattt
     3241 ttatactatg atatttggac ctttaagctg cgtttttgta gttgacaatt taaaaaaaaa
     3301 ctccaaattg ctttgaaaac taccaaaatg agtaccctga taaccatatt gaaaaaccat
     3361 caaatgggta atgtctagaa aaatgaacga aaaattagag aaaataggaa atgagatatg
     3421 ttcttcttcc tattctttat cttctaagct tctcacacct tttctctcgt ctctccacga
     3481 tttacacact atttgcaatg aatctcactc tgatgagcgt atctatcaag tccttgtctc
     3541 ttcttttgac aaaacttcaa agaaatcgcc gacttgcgag gaggggggag cgaaaaataa
     3601 ttgattactg aaatacaaac actgatctta cttgcactta taatacgact cactagtggg
     3661 cagatcttcg aatgcatcgc gcgcaccgta cgtctcgagt gtaaaacgac ggccagtgaa
     3721 ttatcaacta tgtataataa agttgaacga gaaacgtaaa atgatataaa tatcaatata
     3781 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     3841 tcactatggc ggccgcatta ggcaccccca ggctttacac tttatgcttc cggctcgtat
     3901 aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg
     3961 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
     4021 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
     4081 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
     4141 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
     4201 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     4261 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     4321 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     4381 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
     4441 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
     4501 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
     4561 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaatct
     4621 agaggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
     4681 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
     4741 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
     4801 tgtcaatatc tccggttcgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     4861 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
     4921 gaacggctct tttgccgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
     4981 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     5041 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtccccc
     5101 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     5161 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     5221 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta
     5281 tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat
     5341 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     5401 tctcgttcaa cttttctata caaagttgat agcttggcgt aatcatggtc atagctgttc
     5461 ctgcctaggt gagctctggt accctctagt caaggcctta agtgagtcgt attacgtagc
     5521 ccgggcctag ttctagacat tctctaatga aaaaatcttt cagttgaaat tgaaaatgag
     5581 ttaaagttgg agtttttatt gaaaacagat ttccgtgtga ttagtgtttt tagcgagtgt
     5641 gacaggacag cgaaaaaata tagaaacaag gggggaactg aaaagcttag gaatgcattg
     5701 aacatgagaa ggggaagggg aaggaacaaa ctagacagga attattggaa tttaatcaca
     5761 tttggagttt tttttctatt cgacagaata attatccaga acatttttgt attaaatatt
     5821 tatgcatcat atgagtagtc ggctttgttg tgcatgacga gtttgttatc gacgaaatag
     5881 aagctgtcag aacgagtctc gtttggattg ttgatcatgt cgtccactga aaaagagatt
     5941 agtcttttga attgtacttt ttagaataat gactcactga gttgttgaga gagttgaggg
     6001 aactcataga tatgttcaca gttgtttcgt gaattcggaa tacagaatcc gaattcaaag
     6061 tcaaaacact tcagaaggcg atctttgaag aagtgacgtt cgatcattcg gaaatgatgg
     6121 attgggatgt ctcctacttt gaattccaca gttgctccga ccgttttgag tttcaaaaag
     6181 tttggagcaa atctataacg cacgtatctt gccgactcct gtggcgactc atcattctcc
     6241 tgatcgttct ccggtttggc gatctcgaaa agcacttgct cagtgtccag atcacggatt
     6301 tggaacttgg tgaactcgat gttatagatg ttcgcagatg gggagcataa gaatcctaaa
     6361 tttatgtttt aaactgaaat ccaaagggag caagatacct tgagtgattc ccggaagtgc
     6421 taaaacgtcg ttcggagtga tttgagcttt cttcgcaagc tccgattccg ttgtgattcc
     6481 ttgttcggtg cttggtggtg gccgtggcat ctggaaatat ggaaaagttc aacaaaaaga
     6541 aaagagaaaa gaatgaaatc ggatatcaag agttagttga gcggtttctc tagttttctg
     6601 agtctcacct gcgacgggaa ggtcgccgag ccgggtggaa tcgatcgttg ttgctcggct
     6661 ttcatatcgg tttggttgga agcggctgaa aacggaaaga agtggaagaa ggaaaagagt
     6721 gtggtgtgac aggaaaatgg taattagagg gtgccaaata accagctata ttttgttttt
     6781 ttttgaaaac atttttaaaa agaaaaatac gataatgata tcagatggat ttccggaaaa
     6841 ctggtatgaa aaatttcaac ctttttgagt acatgtaatc aaaatacact ttgtaaatta
     6901 tcatttttat tgaaactcca ccatttttct atttataacg ctaataattt gaaaaagaaa
     6961 cctattgcga accgcggggt gaatcccaaa aacgaatgcg ttttggtgga gtgattgatt
     7021 cgaatcgaag aagaaaaaga agaagacgtg gaatagagag ctcactctta accgagcagc
     7081 acacaccgac agaaaaaaaa atgaaatgaa tgagggtctt cttcttcttc ttcttcgaat
     7141 gattgacaga aatgggaaaa agaggaagat tgagaaggga aaaaggaagg agaaaagaag
     7201 cagaagaaga cgtcagagag gagaggaacg agcggaaaag cagcgggcgc aagtcataga
     7261 agtagcagag ctggggagaa gaagacacta tccaagaaag gaatgacgag agagtatgca
     7321 aaggggtata gggtgcagac agaataggaa cagaataaca gatgatgagc caagaagagt
     7381 tgaaaagggc gatgaatttg tcatgtaact taatttgggt caatttgagc atgatgaatt
     7441 gaaatcatcc cttgttggga gttaataacc ggtttgttat cagaaaccct gtaatagaag
     7501 ggcgccctaa ctttgagcca attcatcccg gtttctgtca aatatatcaa aaagtggtca
     7561 actgacaaat tgtttttgat attataataa acattttatc cgttaacaat tttcgaatac
     7621 tttttacaag gacttggata aattggctca aagacgaagt gagtttgcta ccatatttca
     7681 tctttatttt ttaattcgat tttctgatgg caatgactaa ccgattttcg ggataattga
     7741 gatgagggga ggtattttgg gaattgacag ggaggtattt ctgcagattt tgttatcaga
     7801 gtgtactgta gctctaaaac caatacttga cataatactt gaatctatac agttgataag
     7861 tttttttgca gtttcaagtt gaataaagtt atagtggccg acatcttaga ggtctaaaaa
     7921 tgagattttg gtcaaatttg agctagtcca attatacaac taatcgcagt tcttgcgccc
     7981 gacactttac gggatttcaa ttgtatatgt tttgtttcaa ggtttcatct gttcagacaa
     8041 tgcgaagcat tttgttggca ggtttacaaa gttttagcgg ccgacacctc gcgggtttct
     8101 aagataagat ttttgcgaag tcttcagcca tctgtaagct acactagcgg tcgacatctc
     8161 gcgggttaaa tctagctttt gaaaaactat ccaaacacta gaaatattta aattttaatt
     8221 ttaatttttg aaaaaaaatt ccattttaaa ttccggtttt caaaaaaaaa caatagatca
     8281 aaaaatttta acgtattttt ttgcaatttt ttcaaaatgc ataactttca gaatatttct
     8341 cacctcttcg catggtgaaa accgtcaaga gttttttttt caaaataaat ggaaaaatcg
     8401 aaaattttag tttatcaatt tttcgtatat tcgagaaaaa tcttaaaatt ctggtcattt
     8461 ttcttcttct tagaaaaata ttgtatgttt tctttctcat taccataaac aaaatttgat
     8521 aaaatattga attttgctgc tatttttctt aaaatatttt tgtcgatttt cggttttcat
     8581 aaatgtctaa aattcgttgc gtaactttat tgaattatca gcaaaacaaa caaataaaaa
     8641 acgaaaaata tccattttaa aaaattagat aaatgaatca acacgtccct gaatgtaggg
     8701 acgaatgtgc ggaagagact caaaagtctg gcaaaaagtt cggagcctct cgaaatgagc
     8761 cagatagaag ctatcgtagc actgctgaca ccgagttaga aactcgacga cagcgatgtc
     8821 agcccatgaa tatcgatctc caatcatgaa cttttgctcg ccgagcaact cttcatattc
     8881 tctgagcaat gggaagagtg actcctcttt gaatacattc cagcattctt tctgaaaagg
     8941 aagtattgaa ttttatgagg ctatctacct gcctgactac cgcctatttc catgcgcagt
     9001 aggcacatgt gtagacatag gcaggcatta tcgcgcctcc cttcaaacta cctactaggt
     9061 actaggtagt tcgaaaggag acgcgataat gcctgcctat acctacacac gttcctactg
     9121 cacatggaaa taggcggcag gcaggcaggt ggatagcttc aaggtagtgc ctaccctcgc
     9181 gtcatagttc ttcgtcagtg ttgctctcaa aattgggttc atcgctgtgt tgagctcctc
     9241 gagtttttga atcactttat caacttcttc ctgatcccca cttggatcga ctgatcaact
     9301 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     9361 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     9421 ttcccaacag ttgcgcagcc tgaatggcga atggcgcttc gcttggtaat aaagcccgct
     9481 tcggcgggct tttttttgtt aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9541 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9601 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9661 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9721 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9781 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttc tccaatg
//
