LOCUS       sequence-421640-        9950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-Reporter Plasmid for mRNA 3'UTR Massively Parallel Reporter 
            Assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9950)
  TITLE     A systematic search for RNA structural switches across the human 
            transcriptome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMK053
FEATURES             Location/Qualifiers
     source          1..9950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    343..576
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             761..805
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1072..1189
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    1326..1460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1692..2399,2400..2402,2403..2405))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             complement(join(1692..2399,2400..2402,2403..2405))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1731..1752)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2080..2099
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2339..2360
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(2503..2541)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        2569..3079
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2998..3016
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3221..3817
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3221..3240)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3717..3737
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3827..3880
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3893..4600
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4035..4053)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4303..4322
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4620..5208
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4673..4693)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5091..5102)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5295..5528
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5600..5721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5761..5896
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5916..5935)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5917..5935)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5945..5962)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5945..5961)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5954..5976)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6103..6558
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6190..6209)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6400..6421
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6584..6688
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6689..6757,6758..7549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6907..6926)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7720..8308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8209..8228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8462..8479
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8596..8617
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8632..8637,8638..8655,8656..8662)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8670..8686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8675..8697
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8694..8710
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8694..8710
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8729..8749
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8731..8749
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        8850..9229
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9230..9433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9448..9628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9675..9800
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
       61 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      121 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      181 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
      241 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
      301 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
      361 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
      421 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
      481 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
      541 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
      601 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
      661 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
      721 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
      781 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
      841 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
      901 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
      961 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1021 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     1081 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     1141 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcacga
     1201 gactagcctc gagagatctg atcataatca gccataccac atttgtagag gttttacttg
     1261 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     1321 ttgttaactt gtttattgca gcttataatg gttacaaata aggcaatagc atcacaaatt
     1381 tcacaaataa ggcatttttt tcactgcatt ctagttttgg tttgtccaaa ctcatcaatg
     1441 tatcttatca tgtctggatc tcaaatccct cggaagctgc gcctgtctta ggttggagtg
     1501 atacattttt atcactttta cccgtctttg gattaggcag tagctctgac ggccctcctg
     1561 tcttaggtta gtgaaaaatg tcactctctt acccgtcatt ggctgtccag cttagctcgc
     1621 aggggaggtg gtctggatcc accggtccgg tttaattaac gtagcacgcg tttagtcatc
     1681 tagctagcag tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     1741 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
     1801 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     1861 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
     1921 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
     1981 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
     2041 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
     2101 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
     2161 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
     2221 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
     2281 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
     2341 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     2401 accatggtga attccgcgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca
     2461 gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatataggc
     2521 ctcccaccgt acacgcctac cctcgagaag cttgatatcg aattccacgg ggttggggtt
     2581 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     2641 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     2701 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     2761 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     2821 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     2881 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     2941 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     3001 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     3061 caccgacctc tctccccagg gggatccccc gggctgcagg aattcgggcc gcggccagct
     3121 ccggcgggca gggggggcgc tggagcgcag cgcagcgcag ccccatcagt ccgcaaagcg
     3181 gaccgagctg gaagtcgagc gctgccgcgg gaggcgggcg atgaccgagt acaagcccac
     3241 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     3301 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     3361 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     3421 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     3481 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     3541 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     3601 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     3661 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     3721 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     3781 accgcgcacc tggtgcatga cccgcaagcc cggtgccgga tcgggagagg gcagaggaag
     3841 tctgctaaca tgcggtgacg tcgaggagaa tcctggccca ccggtcgcca ccgtgagcaa
     3901 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     3961 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     4021 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     4081 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     4141 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     4201 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     4261 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     4321 gaccatgggc tgggaggcct cctccgagag aatgtacccc gaggacggcg ccctgaaggg
     4381 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     4441 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     4501 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     4561 ccgccactcc accggcggca tggacgagct gtacaagtag agtcgagtct agagtcgaca
     4621 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4681 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4741 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4801 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4861 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4921 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4981 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     5041 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5101 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5161 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag
     5221 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5281 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     5341 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5401 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5461 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5521 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     5581 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     5641 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5701 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     5761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5821 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5881 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     5941 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     6001 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     6061 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     6121 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     6181 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6241 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6301 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6361 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6421 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6481 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     6541 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7861 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8101 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8461 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8521 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     8581 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     8641 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     8701 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     8761 ggagctgcaa gcttggccat tgcatacgtt gtatccatat cataatatgt acatttatat
     8821 tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta
     8881 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     8941 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     9001 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     9061 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     9121 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     9181 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     9241 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     9301 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     9361 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     9421 tataagcaga gctcgtttag tgaaccgggg tctctctggt tagaccagat ctgagcctgg
     9481 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     9541 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     9601 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     9661 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     9721 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     9781 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     9841 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     9901 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
//
