LOCUS       sequence-421657-        2497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 23658 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-Fld
FEATURES             Location/Qualifiers
     source          1..2497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             985..1776
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1867..2455
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2356..2375
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtggg tggaagggct
       61 cggagttgtg gtaatctatg tatcctggtg gtctcgagcg tcgatctcgg accattccag
      121 cacaacagac atcttacact acttgtcata ccgaatttag tcaacgtaat acaaaaactt
      181 ccgaagctgg acggaggaat gtattcacag ttacaccaca atgaagcgtt ctggtgttac
      241 agttacagtt agttgttgtt gccaatggac ttacctgtta gacttagagt tacgactttt
      301 tcgatgcgag gaaaaaagct cgacggtgac gcaggcagca tgattcgtgc ccgatccggc
      361 aaaccccacc ccttaggttc gggtacagca gtagagcatc gtatacgaat tccaaatcag
      421 gttctctact ctctactagg ctagtagcag cgaccccgta tcctaaagag aagagaaggt
      481 caagagtatc cgtaactttg acatttacag ttagcaaata gaagctcctt tagagttaag
      541 ctgagacctg agacgagacg agacagcctg agaatggatg cgagtaatga gctcggcaca
      601 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
      661 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
      721 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
      781 gcgttgctgc ctgtgatcac cgctcctcta gagtcgactt gcaggcatgc aagcttagca
      841 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
      901 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaat atctgatcaa
      961 gagacaggat gaggatcgtt tcgcatgagg gaagcggtga tcgccgaagt atcgactcaa
     1021 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     1081 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     1141 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     1201 acttcggctt cccctggaga gagtgagatt ctccgcgctg tagaagtcac cattgttgtg
     1261 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     1321 cagcgcaatg acattcttgc tggtatcttc gagccagcca cgatcgacat tgatctggct
     1381 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1441 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1501 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1561 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     1621 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     1681 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     1741 gtgaaaggcg agatcaccaa ggtagtcggc aaataataat cacctagaat gcatgaccaa
     1801 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1861 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1921 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1981 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2041 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2101 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2161 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2221 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2281 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2341 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2401 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2461 cagcaacgcg gcctttttac ggttccttgc cttttgc
//
