LOCUS       sequence-42166-e       13631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13631)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 13631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ448 - pDESTR4-R3(ttTi4348, I, unc-18)
FEATURES             Location/Qualifiers
     source          1..13631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..660
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(67..86)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1002..1307
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1186..1205
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1348..1471)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9135..9151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9144..9166)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        9372..9476
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9477..9545,9546..10337)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9695..9714)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(10379..10888)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10574..10595)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10782..10801
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      10999..11587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11488..11507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    11648..11669
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11684..11689,11690..11707,11708..11714)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11722..11738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11727..11749
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11746..11762
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11746..11762
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13375..13391
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    13399..13522
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(13548..13553,13554..13571,13572..13578)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
       61 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
      121 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
      181 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
      241 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
      301 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
      361 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
      421 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
      481 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
      541 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
      601 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
      661 tctagaggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
      721 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta
      781 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
      841 tgatgtcaat atctccggtt cggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
      901 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
      961 aatgaacggc tcttttgccg acgagaacag gggctggtga aatgcagttt aaggtttaca
     1021 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
     1081 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtcc
     1141 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
     1201 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
     1261 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
     1321 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat
     1381 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
     1441 gtttctcgtt caacttttct atacaaagtt gatagcttgg cgtaatcatg gtcatagctg
     1501 ttcctgccta ggtgagctct ggtaccctct agtcaagggg ggcgacccta agctttaatt
     1561 gccaactgtg tcatcaagaa aaacgtattg tcagagcttt tttttttaat tgtgaaattt
     1621 ttgaatttct tttttgattg ttagaaatag ttttaaaagt cttgtattgt ggtgctacat
     1681 tatagatgca caaacagtaa atattgatct aaatttctca aaaagtcaaa aaaatatggt
     1741 tttttatttg gcgtaaaaaa taaacttcga cggataattc ttaaaaatca atattttctg
     1801 tagtatttta ttaaggtggt catgtaagac aaagagtaag cctttgaaaa cttttaattt
     1861 ttatttttat cgccatgaag gaaattgatg tgacattttg gaatatagtc attattgtgt
     1921 tttaattcgt aaaataatta tacaaatagt tattccgtcg agttgatctt tattatgcga
     1981 gtgcaaattc tattcaaaaa atggttggaa caaatggcga gaaacgaaag gaaaatgtac
     2041 caggaaaatc gaggaatgtt tttgaaatgc acaactcttt tgactagaca aaaacacaac
     2101 gtcaaatcac aatccttggt ttttggaatt attgcaacga ggaatcctca ttttcagatt
     2161 aaaagcaaga taaaaaacaa taaaagtcgt agcaacacag aaacactagc ctatgactaa
     2221 atttttttca aaccggggga tacaatcatt agataaaaat aaatactata tctagccgag
     2281 cacattattc aaatagtctt aaagcataaa aatcaaatta tttaaatggt cagtgaaggg
     2341 taatacatgt aactggacga agaaacttat taaaaactat acatatacag ttacattatt
     2401 tttggccatg ggaagagaaa ttaactgaaa ttgtttcggg tttgatagac tgtttttcag
     2461 aagttttgtg aggtaatatc aataaataat gaaggttaat tgagcgcact agctattgtt
     2521 gaaggcttag aaaaatgact tagaggcaac aagctaaatg atgacaaaga ataaattact
     2581 ggtgggagaa taagaaaatt cccaaacgtg tgtggggcac gaaaacaatc tatgagaaaa
     2641 atttactgta atgaaataat ggtcagtgtt tggaaaatgg gagggaaatc tcaaataggg
     2701 ccgtatcttg ttgtttcgtc aattgtcgat tcttttcggt accccgcact ctgtcatatg
     2761 tcacgcggtt tgttcaggtc acgcaagttg gtcaggaact tatccggagt gatgattctg
     2821 tcggacccaa tgacgacttc ccacggtttt cgggcagcgg tcacttcata acaagctcgc
     2881 atttcggaga aggtgacacc tccaataatg tagataatca gtcgtggtcc actgcgatag
     2941 ttggattgtt gtcctcgttc cttgtgccac tgaccatatc tagcagatgc aggggctctg
     3001 aaaatgaaac agggttgaaa tgagcaatca tcagtttttg ctagatcaga gagaagtttt
     3061 gggatgacta atcaatccaa ttgttaaaat tagatacctg tatccttggt taacttgtcg
     3121 tccagcaagg aatgggaaat gttttgtgtc caaacgctcg tcaatagcat cttcaataat
     3181 atccttgata actggaaccc agcgggaaga ttggtaaact tgctcgtgtg gtctttcctt
     3241 tttggttggg gtccacgtct tcttgcggcc ggtctgtaaa ttgacacgtc tatttacata
     3301 tgaaaataca aatccgattt atgcgtactg tttatgttca aaaaaaaaaa cttacatcgg
     3361 taacaatatt caatcccaag taagcagcgt tggtgattgt ttccttatca gccatggaga
     3421 tattagcgtg ttgtaaaagt ttattcaagt tttcatcagt aattccattc ttggaaagaa
     3481 tgtacaacag aatcaatctg aggcggtctt cacaccgcac ggctgggtca atcaaaagtg
     3541 gcaccatcaa cttcattgca tctctgactc gttctccttc tgcgtcgatt ccggtactca
     3601 aatcttgttc aaccttacac aacttgtcaa ctccttgttg atactgtttc atgcattcct
     3661 cagcaagact gatgtgagtg ctaaacttgt tcaactcttt cttgtgctgt ggcattcttt
     3721 tgatgagcat cgatagatcc ttgatcgatt tggagtccat cgttcccttg ttgcccttgc
     3781 tctcactgaa ctttttcaag ttctttgtga cttcttgtga aacaacggca atatgtttgt
     3841 gcctcatttc aacccataaa tcatcattct catcgagcag aacctgcaag aacaaattat
     3901 catccgttat ttgcgtacac tataattgca tgtttcctgg acaacaacat acctctttct
     3961 ccaagttttc atcgcttcct cccgtttcgt acttgtaaac gtcattctca atgcccaaaa
     4021 ggtcgtagca cattgcttga agagtaagtt catggagaag tggagtgatg gcatcgtaac
     4081 ctctgtcgat gatgatcaac tgactgcgtg ccttgtcggc tccctcaccc atagatggat
     4141 cgtcagcctt gtaagcgtcg agtttttgct caacaaggtg gccgagctca acatttctct
     4201 caaagtcagc acggtacctg aaaacttgat tagttttaga aaaactcata tgaaatattt
     4261 tatgtttttt ctttcgttac ttacctcaat gatggatatt ctccaagtgt tgcgcacacc
     4321 gttgcgattt gttcagcaat tctctcaagg ttcgatgtca gaccaccttg tttttgggcg
     4381 ttgtagtata agaaaaatgt atcaggagag tccaaattga aaacctaaaa aagcaaacta
     4441 tatttttgat ttatttggaa caaatctcac ctggctttcg tatggagtga aagctatgtt
     4501 aatctccttc agagttttga tgaaacgagc agctgcactt ttggataatg ttgagaagag
     4561 ctgatccgaa cacgcttcgg tgaaaaagac atgagcgcat ttgtacaaat ttctggcgca
     4621 ataatcttga atcagcttgt ctatctgcaa aacatcttgg tttagacaag cgacttttgg
     4681 agattaaaac taaccgattc agcagttgga gcaatcagat aaatggcttc aagtgttgga
     4741 aggggctctc tccttttgtt tagatcttcc acaattgtaa ttccttctag aaaatagagt
     4801 gattagagaa ggaattaaaa atatcaagtg taattacctt ccataatatt gtgcattttg
     4861 cagcaggatg acaacatccg catggctagg gtgtcaacga tgagaacatt ccacgcgcta
     4921 cgcccgtctc ccttcttcag tggacggatt acatcgttga gcagttcttt aaaagaaaag
     4981 ttttagtaat aattttcaaa gtgtaatttg ttgttttccg tctaacttac tgtgcccaac
     5041 gatttgtttg agtgacatca ctcaaaaatc ctcgtcgatg cactcacaat taacctgcaa
     5101 ccgaaacaaa ttgaattaat cagaagacgc aaaaaagggg agcgaagaac atgagacgga
     5161 aagaaataaa agcatgaata gaatatgggg agtgttgcga ggtttggggc cacccactat
     5221 aagaccaact tgtttctcag gtcagagcgt acaacttcat ggaggtcttg agcacacttc
     5281 atgatttttt cttcttactt cgcatgtgca tgaagactac aaacggcagc aattccaaag
     5341 atggatgcac agaagagata ggaagaaata tgtatgtgtg tgtgtgtcgc gagagaagag
     5401 caatttcgga cttgtcactg acgagtaaag caaaaatcaa taacgtgtaa gcaaacgagg
     5461 gagagagtgt ctgaatctgg tgctcagcat gacacagaga gaggaaaaga gagaagaatt
     5521 ggcaacacga agaggaagag catgccaaat aagaatgttt aacattttgc attgattttt
     5581 tctattttta agaaagttat tataaataaa acgaatattt agaaaactta ttagttttct
     5641 ttgagtaaat ttaattagtt ttttttcttc aaagaccaca taaccaattt ttctgaaaaa
     5701 gttatgttta gattttccaa tgataaaata ccttgttgac aaaatttaaa aataacaatt
     5761 gatatcatca acaaggaaca gtcgatacat agacacatta gggattagga ccgtagacac
     5821 cacggggact gtactgcgta ctatgcgctg gaaatgacgc gcaatggagt gcaatagaga
     5881 ccagcatata actatttcaa tgaaaatggg agattactta tttaataatc ggtatttagg
     5941 ttttataatc tcacatttta aaaaataaac taatttttcc atcaaatttt ccatattaat
     6001 aatacgtgat acacgtgttc ctcttcaagt ttaagtggtt ttccaagaat aaaaatgcaa
     6061 aaggatttcg aacatttcca tttctgataa attaaaaagg tgttatgatt cacaacaagg
     6121 tttaataaat aacaaaatac gtgcgtactt tgaaaacagt tatatatatc taatttttaa
     6181 aattaaagtt catgtatttt gtcgtttaat ttttgacact atatgagcgg gtttaaacag
     6241 ttttcctact gaaggtactc cgtttttaat ttatataaca agaagagcta caaaacactt
     6301 tgtggtttgt ttcaagcaga gaaaatgtga tcacacatat gtactcaaag aaaaaatcac
     6361 aattttagtc tctcttgatg actcagttga ctaaaggcaa aaaagtcagc ctcgtttgca
     6421 caacaacgat tgtgaaatat attccgttgc ctttcttttt tgccagttgc ggaacatttt
     6481 catatgtgtt tgcgccgccg aaaatcgtcc atcattcgaa atatggaaag cgaaacgatg
     6541 tgtctttgta tcttcaaaac ggcatttctc aattgaagct caatggctca ttgacattcg
     6601 aagtgaagac acagtgtagg gtcacggcgc taaaatgcgt gttttcccat tgttttaatg
     6661 gggaaaaagg acaataagag tagaaaacgg caattcgtca aaatgattga ttatattcat
     6721 caattagttt gcaggaaaca acatgcagaa atgtggaaaa gatttcagtg aaagtttcaa
     6781 ttagtttgga aaatcactaa ctgttgaata tgaataaaca aatggagaaa aaacagggat
     6841 gttaaaattt ttatcaaaag tcactcctca aaaaggtatt ccgttgcggg gaatcgaacc
     6901 ccggccttat gggtgagagc cataaatcct aaccactaga ccacaacgga gatgggtgag
     6961 caatggtcgc tgcttaatga taagagtaca gaaaactttg tgactgtgaa caatgtgtag
     7021 aaaattttat cagtcagaaa gcagcacgcg aagaaattga gaaaaatgat ggttgccttt
     7081 ccggcaatct ctttggcaac agttccagaa tttcagctct tattttatat cttttaatca
     7141 gttttcagac tatgatcaaa actttgttgg atgttaaaaa tattggatga ctcgtttctg
     7201 atattaaaaa ttcaaaccag ctgtttatca tcttgtttct cacttccttc aactgctcgt
     7261 ttttgctgtt cgtctatgat tccgcatctt ttgctgttgt aatgtgtgta ttacaccgct
     7321 tccgatgagc agataggagg gccctaactt tgagccaatt catcccggtt tctgtcaaat
     7381 atatcaaaaa gtggtcaact gacaaattgt ttttgatatt ataataaaca ttttatccgt
     7441 taacaatttt cgaatacttt ttacaaggac ttggataaat tggctcaaag acgaagtgag
     7501 tttgctacca tatttcatct ttatttttta attcgatttt ctgatggcaa tgactaaccg
     7561 attttcggga taattgagat gaggggaggt attttgggaa ttgacaggga ggtatttctg
     7621 cagattttgt tatcagagtg tactgtagct ctaaaaccaa tacttgacat aatacttgaa
     7681 tctatacagt tgataagttt ttttgcagtt tcaagttgaa taaagttata gtggccgaca
     7741 tcttagaggt ctaaaaatga gattttggtc aaatttgagc tagtccaatt atacaactaa
     7801 tcgcagttct tgcgcccgac actttacggg atttcaattg tatatgtttt gtttcaaggt
     7861 ttcatctgtt cagacaatgc gaagcatttt gttggcaggt ttacaaagtt ttagcggccg
     7921 acacctcgcg ggtttctaag ataagatttt tgcgaagtct tcagccatct gtaagctaca
     7981 ctagcggtcg acatctcgcg ggttaaatct agcttttgaa aaactatcca aacactagaa
     8041 atatttaaat tttaatttta atttttgaaa aaaaattcca ttttaaattc cggttttcaa
     8101 aaaaaaacaa tagatcaaaa aattttaacg tatttttttg caattttttc aaaatgcata
     8161 actttcagaa tatttctcac ctcttcgcat ggtgaaaacc gtcaagagtt tttttttcaa
     8221 aataaatgga aaaatcgaaa attttagttt atcaattttt cgtatattcg agaaaaatct
     8281 taaaattctg gtcatttttc ttcttcttag aaaaatattg tatgttttct ttctcattac
     8341 cataaacaaa atttgataaa atattgaatt ttgctgctat ttttcttaaa atatttttgt
     8401 cgattttcgg ttttcataaa tgtctaaaat tcgttgcgta actttattga attatcagca
     8461 aaacaaacaa ataaaaaacg aaaaatatcc attttaaaaa attagataaa tgaatcaaca
     8521 cgtccctgaa tgtagggacg aatgtgcgga agagactcaa aagtctggca aaaagttcgg
     8581 agcctctcga aatgagccag atagaagcta tcgtagcact gctgacaccg agttagaaac
     8641 tcgacgacag cgatgtcagc ccatgaatat cgatctccaa tcatgaactt ttgctcgccg
     8701 agcaactctt catattctct gagcaatggg aagagtgact cctctttgaa tacattccag
     8761 cattctttct gaaaaggaag tattgaattt tatgaggcta tctacctgcc tgactaccgc
     8821 ctatttccat gcgcagtagg cacatgtgta gacataggca ggcattatcg cgcctccctt
     8881 caaactacct actaggtact aggtagttcg aaaggagacg cgataatgcc tgcctatacc
     8941 tacacacgtt cctactgcac atggaaatag gcggcaggca ggcaggtgga tagcttcaag
     9001 gtagtgccta ccctcgcgtc atagttcttc gtcagtgttg ctctcaaaat tgggttcatc
     9061 gctgtgttga gctcctcgag tttttgaatc actttatcaa cttcttcctg atccccactt
     9121 ggatcgactg atcaactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9181 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9241 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcttcgct
     9301 tggtaataaa gcccgcttcg gcgggctttt ttttgttaac tacgtcaggt ggcacttttc
     9361 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9421 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9481 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9541 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9601 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     9661 aacgttctcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     9721 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     9781 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     9841 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     9901 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     9961 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10021 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    10081 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    10141 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10201 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    10261 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10321 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttac
    10381 cccggttgat aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat
    10441 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
    10501 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaatagc ccgagatagg
    10561 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
    10621 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc
    10681 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
    10741 atttagagct tgacggggaa agcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
    10801 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
    10861 gcgcttaatg cgccgctaca gggcgcgtaa aaggatctag gtgaagatcc tttttgataa
    10921 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10981 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    11041 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    11101 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    11161 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    11221 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    11281 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    11341 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    11401 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    11461 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    11521 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    11581 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    11641 tcacatgtaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    11701 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    11761 accatgggtc accaggtatc gcgctttaat tagggtgcaa gttttttata atttttaatc
    11821 ccattaaaat tcaaagcaga aaggttctga tgctctacgc aaaatatcaa aatcacaatt
    11881 tatgacaata ttgttacaaa aatccaaaaa aaaaattgca gttcgaaatg gtgattgaag
    11941 aaaacggatt acgtgaagct actcgacgaa tcatatccta tttggaaaaa tatattcatg
    12001 cactgcacta tcatcataag gatgatgaga tttaataatt tttgtaattt atcttacaat
    12061 tagcatttta tttaatttta aaaaaatttg aatgggaaca taaagtcaaa ttcctgagct
    12121 gcatggctaa gggtgccctc gaaaattttc cgattgttcg atttatttca gagaaattcg
    12181 gaaaatgcga aatcgaaaaa aagtgtagaa aatcaaaaaa tgcaaaaatt tataccaaac
    12241 cgtaaaaaat taccaaaatt taaagatatt ttacggaaac ttacgcttga gttattcaaa
    12301 cacataactg atattataac attaatacta tattaatact agattaatat gtactatagc
    12361 aattttcaaa atattttcaa ttagcaaaat gttgccgatc atttttctac atttacaatt
    12421 aaaattaaaa tttgataacc ctgatccgga aaaagttacg gcagctgaca tttgtctgat
    12481 ttttgactag aatcgctaag tggcccacaa tttttaaact aggtgttttc acgaaacaaa
    12541 gttttctatt tttttgcaat tttttatcct gtcacaaaag gaccataaaa attttataca
    12601 aaagattaaa catgaaactc aaatttcaaa aattagagtg tttcgtaaaa agtaacgtac
    12661 atatggtgcc aaaatggcca aatatcatag taaaattttt caaaacattt ttgaaaaaag
    12721 tggcaattac tgagtttttg ccactttttt ttagtaaact atagctctct cgcatactcg
    12781 aattcctaca aaaatttata tttgaaatat aagtaccata aaatccggag aaaaacttcc
    12841 aaaaaaatgg caaaaactaa cattcttcca attttcaaaa aatcatataa aatttggaat
    12901 tttttaaaga atttttatac tatgatattt ggacctttaa gctgcgtttt tgtagttgac
    12961 aatttaaaaa aaaactccaa attgctttga aaactaccaa aatgagtacc ctgataacca
    13021 tattgaaaaa ccatcaaatg ggtaatgtct agaaaaatga acgaaaaatt agagaaaata
    13081 ggaaatgaga tatgttcttc ttcctattct ttatcttcta agcttctcac accttttctc
    13141 tcgtctctcc acgatttaca cactatttgc aatgaatctc actctgatga gcgtatctat
    13201 caagtccttg tctcttcttt tgacaaaact tcaaagaaat cgccgacttg cgaggagggg
    13261 ggagcgaaaa ataattgatt actgaaatac aaacactgat cttacttgca cttataatac
    13321 gactcactag tgggcagatc ttcgaatgca tcgcgcgcac cgtacgtctc gagtgtaaaa
    13381 cgacggccag tgaattatca actatgtata ataaagttga acgagaaacg taaaatgata
    13441 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
    13501 cacaacatat ccagtcacta tggcggccgc attaggcacc cccaggcttt acactttatg
    13561 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
    13621 aggaagctaa a
//
