LOCUS       sequence-421668-       15472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses betalain biosynthesis genes under CaMV 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15472)
  AUTHORS   Lee K, Kang M, Ji Q, Grosic S, Wang K
  TITLE     New T-DNA binary vectors with NptII selection and RUBY reporter for 
            efficient maize transformation and targeted mutagenesis.
  JOURNAL   Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231.
  PUBMED    37070560
REFERENCE   2  (bases 1 to 15472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231."
COMMENT     Sequence Label: pCBL102-RUBY
FEATURES             Location/Qualifiers
     source          1..15472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    183..204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(219..224,225..242,243..249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    257..273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     262..284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     281..297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     281..297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        332..1008
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     945..965
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1017..4967
                     /label=RUBY
     CDS             2517..2573
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3408..3464
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(8680..8697)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8680..8696)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8689..8711)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8911..8935
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9456..10247
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10493..11081
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10982..11001
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11235..11252
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11267..11407
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11333..11352)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11493..11515
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11751..11945
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12011..13084)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13513..14142)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
      301 attacgaatt cgagctcgac actgatagtt ttgagacttt tcaacaaagg gtaatatcgg
      361 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
      421 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
      481 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
      541 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgaacatggt ggagcacgac
      601 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
      661 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
      721 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
      781 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca
      841 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
      901 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
      961 ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa actagtatgg
     1021 atcatgcgac cctcgccatg atcctcgcga tctggttcat cagcttccac ttcatcaagc
     1081 tgctgttctc ccagcagacc accaagctgc ttccgccagg accaaagccg cttccgatca
     1141 tcggcaacat ccttgaggtg ggcaagaagc cgcatcggtc cttcgccaac ctcgccaaga
     1201 ttcacggccc actcatttcc ctcagactcg gctctgtgac caccatcgtt gtgtcctctg
     1261 ccgacgtggc caaagagatg ttcctcaaga aggatcaccc gctctccaac cgcacgatcc
     1321 cgaatagtgt tacagccggc gaccaccaca agctcaccat gtcttggctc ccggtgtctc
     1381 cgaagtggcg caacttccgc aagattaccg ccgtgcatct gctctcccca cagagactcg
     1441 atgcctgcca gacattcagg cacgccaagg tgcagcagct ctacgagtac gttcaagagt
     1501 gcgcccagaa aggccaggcc gtggatattg gcaaggccgc ctttacgacc agcctcaacc
     1561 tcctcagcaa gctgttcttc agcgtcgagc tggcgcacca caagtcccat accagccaag
     1621 agttcaaaga gctgatctgg aacatcatgg aagatatagg caagccgaac tacgccgact
     1681 acttcccgat tctcggctgc gttgacccat ctggcattag aagaaggctc gcctgctcct
     1741 tcgacaagct gatcgccgtg ttccagggca tcatctgcga gagactcgcc ccagattcct
     1801 ccaccacaac taccaccacc accgacgacg tgctcgatgt gctcctccag ctgttcaagc
     1861 agaacgagct gacgatgggc gagatcaacc acctcctcgt ggacatcttc gacgccggca
     1921 ccgataccac atcctccaca ttcgagtggg tgatgaccga gctgatccgc aatccagaga
     1981 tgatggaaaa ggcccaagag gaaatcaagc aggtcctcgg caaggacaag cagatccaag
     2041 agtccgacat catcaacctg ccgtacctcc aggcgatcat caaagagaca ctccgcctcc
     2101 atccgccgac cgtgttcttg ctcccaagaa aggccgacac cgatgtcgag ctgtacggct
     2161 acatcgtgcc gaaggatgcc cagatcctcg tgaacctctg ggccattggc agggacccaa
     2221 acgcctggca gaacgccgat attttcagcc cagagcgctt catcggctgc gagatcgatg
     2281 ttaagggccg cgatttcggc ctccttccat ttggcgctgg ccgcagaatt tgcccaggca
     2341 tgaatctcgc catcaggatg ctcaccctca tgctcgccac actcctccag ttcttcaact
     2401 ggaagctcga aggcgacatc tccccgaagg acctcgacat ggacgagaag ttcggcattg
     2461 cgctccaaaa gaccaagccg ctcaagctca tcccgattcc gcgctacggt agcggagcta
     2521 ccaattttag cctccttaag caggcaggtg atgtagaaga gaaccccggg cctatgaaga
     2581 tgatgaacgg cgaggacgcc aacgaccaga tgatcaaaga gtccttcttc atcacccacg
     2641 gcaacccgat cctcaccgtc gaggatacac atccgctcag gccgttcttc gagacatggc
     2701 gcgagaagat tttctccaag aagccgaagg ccatcctcat catctccggc cactgggaga
     2761 cagtgaagcc aaccgtgaac gccgtgcaca tcaacgacac catccacgac ttcgacgact
     2821 acccagccgc catgtaccag ttcaagtacc cagctccagg cgagccagag cttgcgagaa
     2881 aggtggaaga gatcctcaag aagtccgggt tcgagacagc cgagacagac caaaagaggg
     2941 gccttgatca cggcgcctgg gttccactca tgctcatgta tccagaggcg gacatcccgg
     3001 tgtgccagct ctcagttcag ccacatctcg acggcaccta ccactacaat ctcggcagag
     3061 ccctcgcgcc gctcaagaat gatggcgtgc tcattattgg ctccggcagc gccacacatc
     3121 cactcgatga gacaccgcac tacttcgatg gtgttgcccc ttgggccgct gccttcgatt
     3181 cttggcttag gaaggccctc atcaacggcc gcttcgagga agtgaacatc tacgagagca
     3241 aggccccgaa ctggaagctc gcccatccat ttccagagca cttctacccg ctccacgttg
     3301 tgctcggcgc tgctggtgaa aagtggaagg ccgagctgat ccactcctcc tgggatcatg
     3361 gcacactttg ccacggctcc tacaagttca cctccgccgg atccggagca accaacttta
     3421 gcctgctcaa gcaagcagga gatgttgagg aaaatcctgg ccccatgacc gccatcaaga
     3481 tgaacaccaa cggcgagggc gagacacagc acatcctcat gatcccgttc atggcgcagg
     3541 gccacctcag gccatttctc gaactcgcca tgttcctcta caagcgctcc cacgtgatca
     3601 tcaccctgct cacaactccg ctcaatgccg gcttcctcag gcacctcctt caccaccatt
     3661 cctactcctc cagcggcatc aggatcgtcg agctgccatt caactccacc aaccacggac
     3721 tcccaccggg catcgagaac accgataagc tcacactccc gctcgtggtg tccctcttcc
     3781 attccaccat cagcctcgat ccgcacctcc gcgattacat ctccaggcat ttcagcccag
     3841 ccaggccacc actctgcgtg atccatgatg tgttcctcgg ctgggttgac caggtggcca
     3901 aggatgtggg ctctacaggc gtggtgttca caacaggcgg cgcttatggc acatccgcct
     3961 acgtgtccat ctggaacgat ctcccgcacc agaactactc cgacgaccaa gagttcccgc
     4021 tgccaggctt cccagagaac cataagttcc gcaggtccca gctccatcgg ttcctcagat
     4081 atgccgacgg ctccgacgat tggtccaagt atttccagcc gcagctccgc cagtccatga
     4141 agtcttttgg ctggctctgc aactccgtgg aagagatcga gacactcggc ttctccatcc
     4201 tccgcaacta caccaagctg ccgatctggg gcatcggccc acttattgct tccccagtgc
     4261 agcactcctc ctccgacaac aattcaacag gcgccgagtt cgtgcagtgg ctcagcctca
     4321 aagagccgga ctccgtcctc tacatctcct tcggctccca gaacacgatc agcccgacgc
     4381 agatgatgga actcgctgct ggccttgagt cctccgagaa gccattcctc tgggtgatca
     4441 gagccccgtt cggcttcgac atcaacgaag agatgcgccc agagtggctg ccagagggct
     4501 ttgaggaacg catgaaggtg aagaaacagg gcaagctcgt gtacaagctc ggcccgcagc
     4561 ttgagatcct caaccatgaa tccatcggcg gctttctcac ccactgcgga tggaacagca
     4621 tccttgagtc tcttcgcgag ggcgttccga tgcttggatg gccacttgct gccgagcagg
     4681 cctacaacct caagtacctc gaagatgaga tgggcgtcgc ggttgagctt gctagaggcc
     4741 tcgaaggcga gatctccaaa gagaaggtca agcgcatcgt cgagatgatc cttgagcgca
     4801 acgagggctc caaaggctgg gagatgaaga atcgcgccgt ggaaatgggc aaaaagctca
     4861 aggacgccgt gaacgaggaa aaagagctga agggctcctc cgtgaaggcg atcgacgatt
     4921 tcctcgacgc cgtcatgcag gccaaacttg agccaagcct ccagtgatag tgaatatgaa
     4981 gatgaagatg aaatatttgg tgtgtcaaat aaaaaggttg tgtgcttaag tttgtgtttt
     5041 tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaacat
     5101 ctcattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc
     5161 ttctccagca tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat
     5221 aagacctaac tgagtaagct agcttgttcg agtattatgg cattgggaaa actgttttcc
     5281 ggggatcctc tagagtcgac ctgcaggcat gcagccctta ctttaacgcc tctaaccaac
     5341 acccctttat ctttataagg aacaataaac agaatttgcc ccactgttct aaatcaccta
     5401 ataatatccc cagctaaaaa caataaaggt ttcctagaat taagacaagc atgactgttc
     5461 ctccaggagg gtttggaaca ttgttgcagt cttgcagata cgggcgaagg gtgagaaaca
     5521 gagcggaggg ctggaggtga cctcggtagt cgacgccgga gttgagcttg acaacgacgg
     5581 ggcggcccct gatggacttg aggaagtccg atggcgtctt caccgtcccg ccggcgcccg
     5641 aggcgggcct gtcgctgccg ccgccgccgc tgctcatctt gcgcgctgtg cccccggcgg
     5701 tgtccctgtg ttgcggatcg cgggtgggcc aggtggatgc gagggcgacc cgtttggact
     5761 ccggccggag ccgccggatc cctggtcggt gtcagtgccg tttactctgg gccccacgtg
     5821 tcagtaccgt ctgtagatga caacaacccg tcgtccacag tcatgtccaa aatatccttt
     5881 cttctttttt ttcgattcgg atatctatct tccttttttt tttccaaaaa tcttcttgac
     5941 gcaccagcgc gcacgtttgt ggtaaacgcc gacacgtcgg tcccacgtcg atagacccca
     6001 cccaccagtg agtagcgtgt acgtattcgg gggtgacgga cgtgtcgccg tcgtcttgct
     6061 agtcccattc ccatctgagc cacacatctc tgaacaaaaa aaaggaggga ggcctccacg
     6121 cacatccccc tccgtgccac ccgccccaaa ccctcgcgcc gcctccgaga cagccgccgc
     6181 aaccatggcc accgccgccg ccgcgtctac cgcgctcact ggcgccacta ccgctgcgcc
     6241 caaggcgagg cgccgggcgc acctcctggc cacccgccgc gccctcgccg cgcccatcag
     6301 gtgctcagcg gcgtcacccg ccatgccgat ggctcccccg gccaccccgc tccggccgtg
     6361 gggccccacc gagccccgca agggtgctga catcctcgtc gagtccctcg agcgctgcgg
     6421 cgtccgcgac gtcttcgcct accccggcgg cgcgtccatg gagatccacc aggcactcac
     6481 ccgctccccc gtcatcgcca accacctctt ccgccacgag caaggggagg cctttgccgc
     6541 ctccggctac gcgcgctcct cgggccgcgt cggcgtctgc atcgccacct ccggccccgg
     6601 cgccaccaac ctagtctccg cgctcgccga cgcgctgctc gattccgtcc ccatggtcgc
     6661 catcacggga caggtggcgc gacgcatgat tggcaccgac gccttccagg agacgcccat
     6721 cgtcgaggtc acccgctcca tcaccaagca caactacctg gtcctcgacg tcgacgacat
     6781 cccccgcgtc gtgcaggagg ctttcttcct cgcctcctct ggtcgaccag ggccggtgct
     6841 tgtcgacatc cccaaggaca tccagcagca gatggcggtg cctgtctggg acaagcccat
     6901 gagtctgcct gggtacattg cgcgccttcc caagccccct gcgactgagt tgcttgagca
     6961 ggtgctgcgt cttgttggtg aatcgcggcg ccctgttctt tatgtgggcg gtggctgcgc
     7021 agcatctggt gaggagttgc gacgctttgt ggagctgact ggaatcccgg tcacaactac
     7081 tcttatgggc ctcggcaact tccccagcga cgacccactg tctctgcgca tgctaggtat
     7141 gcatgggacg gtgtatgcaa attatgcagt ggataaggcc gatctgttgc ttgcacttgg
     7201 tgtgcggttt gatgatcgcg tgacagggaa gattgaggct tttgcaagca gggctaagat
     7261 tgtgcacgtt gatattgatc cggctgagat tggcaagaac aagcagccac atgtgtccat
     7321 ctgtgcagat gttaagcttg ctttgcaggg catgaatgct cttcttgaag gaagcacatc
     7381 aaagaagagc tttgactttg gctcatggaa cgatgagttg gatcagcaga agagggaatt
     7441 cccccttggg tataaaacat ctaatgagga gatccagcca caatatgcta ttcaggttct
     7501 tgatgagctg acgaaaggcg aggccatcat cggcacaggt gttgggcagc accagatgtg
     7561 ggcggcacag tactacactt acaagcggcc aaggcagtgg ttgtcttcag ctggtcttgg
     7621 ggctatggga tttggtttgc cggctgctgc tggtgcttct gtggcaaacc caggtgtcac
     7681 tgttgttgac atcgatggag atggtagctt tctcatgaac gttcaggagc tagctatgat
     7741 ccgaattgag aacctcccag tgaaggtctt tgtgctaaac aaccagcacc tggggatggt
     7801 ggtgcagttg gaggacaggt tctataaggc caacagagcg cacacatact tgggaaaccc
     7861 agagaatgaa agtgagatat atccagattt cgtgacgatc gccaaagggt tcaacattcc
     7921 agcggtccgt gtgacaaaga agaacgaagt ccgcgcagcg ataaagaaga tgctcgagac
     7981 tccagggccg tacctcttgg atataatcgt cccacaccag gagcatgtgt tgcctatgat
     8041 ccctagtggt ggggctttca aggatatgat cctggatggt gatggcagga ctgtgtactg
     8101 actagctagt cagttaacct agacttgtcc atcttctgga ttggccaact taattaatgt
     8161 atgaaataaa aggatgcaca catagtgaca tgctaatcac tataatgtgg gcatcaaagt
     8221 tgtgtgttat gtgtaattac tagttatctg aataaaagag aaagagatca tccatatttc
     8281 ttatcctaaa tgaatgtcac gtgtctttat aattctttga tgaaccagat gcatttcatt
     8341 aaccaaatcc atatacatat aaatattaat catatataat taatatcaat tgggttagca
     8401 aaacaaatct agtctaggtg tgttttgcga atgcggccgc ctgcagagtt aacggcgcgt
     8461 cgtctattgg accgacagtt gctattaata taatgggcca ccatagtaga ctgacaaata
     8521 aattacctga caacatcgtt tcacaaaaaa acaaacacaa aaagggagtg cattttccag
     8581 ggcatttttg taataaaaaa cagttaaaag ggagtgcaat agaaatatag gggtgtggaa
     8641 atagtgattt gagcacgtct tgaagcgaat tagcttggca ctggccgtcg ttttacaacg
     8701 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     8761 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     8821 cctgaatggc gaatgctaga gcaattcggc gttaattcag tacattaaaa acgtccgcaa
     8881 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     8941 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     9001 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     9061 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     9121 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg
     9181 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     9241 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     9301 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     9361 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     9421 agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag
     9481 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     9541 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     9601 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     9661 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     9721 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     9781 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     9841 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     9901 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     9961 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
    10021 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
    10081 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
    10141 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
    10201 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa
    10261 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag
    10321 cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg
    10381 cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg
    10441 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10501 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10561 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10621 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10681 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10741 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10801 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10861 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10921 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10981 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    11041 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    11101 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    11161 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    11221 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
    11281 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
    11341 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
    11401 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
    11461 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
    11521 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
    11581 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
    11641 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
    11701 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
    11761 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
    11821 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
    11881 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
    11941 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
    12001 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
    12061 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
    12121 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
    12181 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
    12241 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
    12301 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
    12361 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
    12421 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
    12481 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
    12541 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
    12601 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
    12661 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
    12721 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
    12781 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
    12841 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
    12901 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
    12961 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
    13021 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
    13081 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca
    13141 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
    13201 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
    13261 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
    13321 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
    13381 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
    13441 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
    13501 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
    13561 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    13621 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    13681 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    13741 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
    13801 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
    13861 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
    13921 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    13981 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    14041 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    14101 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    14161 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    14221 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    14281 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    14341 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    14401 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    14461 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    14521 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    14581 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    14641 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    14701 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    14761 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    14821 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    14881 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    14941 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    15001 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    15061 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    15121 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    15181 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    15241 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    15301 agcagcggag gggttggatc aaagtacttt aaagtacttt aaagtacttt aaagtacttt
    15361 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt
    15421 ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt ag
//
