LOCUS       sequence-42167-e        7517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ350 - MCS(ttTi5605, II)
FEATURES             Location/Qualifiers
     source          1..7517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1559..1579
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3007..3031
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3038..3054
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3038..3054
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3250..3267)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3250..3266)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5207..5218
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5364..5388)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6726..6746)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6950..6969)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7069..7091
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7129..7147)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7215..7319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7320..7388,7389..7517,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccat cgaattatca
     1561 actatgtata ataaagttgt gtttttgaac acggcgatat gtatctgtag atcatcccca
     1621 attttcaaca atgaatctaa atactgaact ttttgtgtaa tctttagtgc agaaaaatga
     1681 ccaaaatcag cgattttccg attataccca aaatatctca gctcaatgag cacctataaa
     1741 aatgtaaaaa taaagaaagt agtcgaacca tattttgtaa aggctaaaaa actgaataaa
     1801 tttttatcca tccagcgcgg cgttacaatc cgtggcccat gtcacgcacg gctttcaaag
     1861 aaaaaaaaac agtctgtttg cgcgacatcg atctggcaaa aatcctaaaa acgactatgc
     1921 gtggctcact ttatattaat atgaaaaata caaacttggt ggtgcaggtg ctgcagaggc
     1981 catcgatgaa gaagtggagg ccttgttgtc gatctacaaa attgtatggt tgtttgagat
     2041 tttccttgaa acgctctcaa tcttggatat gatataaatt ctggataaga tatgatggat
     2101 atgatctatg tttgatagag tgcatttgaa aggataacgg ccctgcagac atttttgaaa
     2161 aaataacaaa catttaattt tctcactcgt ttaggctatt cccctatttt tgatattcct
     2221 tcgcacatat gaaaactact ttttttcgaa actgttaact ccagaattta taaatctata
     2281 gcccttactt gattatttat tatcatggtt actcacctca tgtctctttc tcttttcaag
     2341 aagtcttcgt tcatatctgt tgtctctttc ctccttttga atctctgctt tgctcttcgc
     2401 cattgtttcc tgaaaataat gtaacttgaa ttgtgtaata tactttttta atttgaattt
     2461 ggcttgtaac gcggaatcac tacgtgcggg atcatttctt actagaaaac ccagaaaatg
     2521 ccatatttca cttatctcgg ggtcatttct aattagaaaa gctacaaaaa ccatttctaa
     2581 ttacggtgac tttagaactg gtcgaaatag ctaattagaa atgggttttt gctaattaga
     2641 ggtgactcta tgttatctga gtaagcctcg tttgtatgta aactcacagt actaataaat
     2701 gcaagacacc cgggtttgtc tagatatgaa ataattgaaa tatcaattct gacagacaat
     2761 aatggtaatc ttgataagga gttccacgcc caggagaaca cgttagtttt cttgtttttg
     2821 attgcgtgcg ttattttgga gaaaaactcg attttttaca aaataatttt ttgaaaggaa
     2881 cactgttcaa taagttttgt cttttttctc agttgtgata cggtttttta ttcttttttg
     2941 tagttataca gaagaccgtt acgaaacaga ctgatatcga aacaaacact gatcttactt
     3001 gcacttacca ctttgtacaa gaaagctggg tcacgggcag gaaacagcta tgaccatgat
     3061 tacgccaagc tacgtaatac gactcactag tgggcagatc ttcgaatgca tcgcgcgcac
     3121 cgtacgtctc gaggaattcc tgcaggatat ctggatccac gaagcttccc atggtgacgt
     3181 caccggttct agatacctag gtgagctctg gtaccctcta gtcaaggcct taagtgagtc
     3241 gtattacgga ctggccgtcg ttttacaccc gtgcagttga aattgaaaat gagttaaagt
     3301 tggagttttt attgaaaaca gatttccgtg tgattagtgt ttttagcgag tgtgacagga
     3361 cagcgaaaaa atatagaaac aaggggggaa ctgaaaagct taggaatgca ttgaacatga
     3421 gaaggggaag gggaaggaac aaactagaca ggaattattg gaatttaatc acatttggag
     3481 ttttttttct attcgacaga ataattatcc agaacatttt tgtattaaat atttatgcat
     3541 catatgagta gtcggctttg ttgtgcatga cgagtttgtt atcgacgaaa tagaagctgt
     3601 cagaacgagt ctcgtttgga ttgttgatca tgtcgtccac tgaaaaagag attagtcttt
     3661 tgaattgtac tttttagaat aatgactcac tgagttgttg agagagttga gggaactcat
     3721 agatatgttc acagttgttt cgtgaattcg gaatacagaa tccgaattca aagtcaaaac
     3781 acttcagaag gcgatctttg aagaagtgac gttcgatcat tcggaaatga tggattggga
     3841 tgtctcctac tttgaattcc acagttgctc cgaccgtttt gagtttcaaa aagtttggag
     3901 caaatctata acgcacgtat cttgccgact cctgtggcga ctcatcattc tcctgatcgt
     3961 tctccggttt ggcgatctcg aaaagcactt gctcagtgtc cagatcacgg atttggaact
     4021 tggtgaactc gatgttatag atgttcgcag atggggagca taagaatcct aaatttatgt
     4081 tttaaactga aatccaaagg gagcaagata ccttgagtga ttcccggaag tgctaaaacg
     4141 tcgttcggag tgatttgagc tttcttcgca agctccgatt ccgttgtgat tccttgttcg
     4201 gtgcttggtg gtggccgtgg catctggaaa tatggaaaag ttcaacaaaa agaaaagaga
     4261 aaagaatgaa atcggatatc aagagttagt tgagcggttt ctctagtttt ctgagtctca
     4321 cctgcgacgg gaaggtcgcc gagccgggtg gaatcgatcg ttgttgctcg gctttcatat
     4381 cggtttggtt ggaagcggct gaaaacggaa agaagtggaa gaaggaaaag agtgtggtgt
     4441 gacaggaaaa tggtaattag agggtgccaa ataaccagct atattttgtt tttttttgaa
     4501 aacattttta aaaagaaaaa tacgataatg atatcagatg gatttccgga aaactggtat
     4561 gaaaaatttc aacctttttg agtacatgta atcaaaatac actttgtaaa ttatcatttt
     4621 tattgaaact ccaccatttt tctatttata acgctaataa tttgaaaaag aaacctattg
     4681 cgaaccgcgg ggtgaatccc aaaaacgaat gcgttttggt ggagtgattg attcgaatcg
     4741 aagaagaaaa agaagaagac gtggaataga gagctcactc ttaaccgagc agcacacacc
     4801 gacagaaaaa aaaatgaaat gaatgagggt cttcttcttc ttcttcttcg aatgattgac
     4861 agaaatggga aaaagaggaa gattgagaag ggaaaaagga aggagaaaag aagcagaaga
     4921 agacgtcaga gaggagagga acgagcggaa aagcagcggg cgcaagtcat agaagtagca
     4981 gagctgggga gaagaagaca ctatccaaga aaggaatgac gagagagtat gcaaaggggt
     5041 atagggtgca gacagaatag gaacagaata acagatgatg agccaagaag agttgaaaag
     5101 ggcgatgaat ttgtcatgta acttaatttg ggtcaatttg agcatgatga attgaaatca
     5161 tcccttgttg ggagttaata accggtttgt tatcagaaac cctgtaatag aagggcgccc
     5221 taactttgag ccaattcatc ccggtttctg tcaaatatat caaaaagtgg tcaactgaca
     5281 aattgttttt gatattataa taaacatttt atccgttaac aattttcgaa tactttttac
     5341 aaggacttgg ataaattggc tcaagcctgc ttttttgtac aaacttgacg aagtgagttt
     5401 gctaccatca tggaatcagg aggacatttc gacaatgtca tataatgttt gaaaaatgat
     5461 tttaacaagg gtctcattcg agataagcaa tgctctgagc aagtggctcg tcgtcttcaa
     5521 gaatctcttg ttgatattca tttggatcct gttttgcacg aagcatgtgc catggatatt
     5581 cagagttttt gcaaaaaagt tccgccaggt caaacaatct ttttaatttt tatatgatac
     5641 ttcgggtgta tttttcttct ttttcctgtt cattttcgct tttttctcac ttttttgtaa
     5701 caaaatctct cacttttctg taagtcttaa caaaagttca attggctcga tcaagtaaaa
     5761 tttcggccgt atgaacgttt tcaaaggttt tcattttttt ttaagttcat tcacaatatc
     5821 atgaatctct cgtcctttgg tttccggtaa gtagcgaaat aatataagca tacttataaa
     5881 tgaaggaatt gaatatagaa taaggaaagc ataactacca ataacggaat atagaggtaa
     5941 tactgtaaat gtactgataa ccaccattac agtattcatt gaatatgcca cagattgagc
     6001 aatactacga tgtttttgag gtaccagttc agaggatata aaccacgaaa ttggaccaac
     6061 tccagttctg aaatttcatt attcgattaa atcaaaatat atttgaaaaa cctcacccat
     6121 aagtataacc gtagatgatc aatgcaacta ggcatccgta tttcatcggg tcaatcagaa
     6181 tacgtatttt tgcgaagaat acgaatgcaa ttagagctaa attattgaga aatgtgaaga
     6241 gaaggagaag cttgcgtcga ccaactctgt aaacttattc atacaatttt gtcttcaaat
     6301 tttgaacgtt taaaactatc ttattcaatg agttagcggg tttttatagt gagttagttc
     6361 acgattatct aataaaacta tgtccaaaca tcctaactgc gaccgtattc tcactaactc
     6421 gtcttaaaaa cgttgagatt attttaaacc cttcttcagt acaactacag tactgatgca
     6481 caaatacaat aataataata cattcagaca tactctgaac aatgtaactt gtttttgtta
     6541 tactgtggta acatttattg aaatgaaata taaccttact tttcaatcac aattccacca
     6601 gtaattgttc cagaaacata agctaaaccc attgcagttg tactccattc agcaatttct
     6661 gactcaacat ttgcattttc tagaaagaaa gtagaggata aaagaagagc ccataatccg
     6721 acaagcaact tttctataca aagttgatag cttggcggat ccctggcgtt acccaactta
     6781 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     6841 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     6901 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     6961 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7021 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7081 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7141 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7201 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7261 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7321 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7381 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7441 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7501 aagaacgttt tccaatg
//
