LOCUS       sequence-42168-e        7265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ351 - MCS(cxTi10882, IV)
FEATURES             Location/Qualifiers
     source          1..7265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1559..1579
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2629..2653
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2660..2676
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2660..2676
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2872..2889)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2872..2888)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4829..4840
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4986..5010)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6474..6494)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6698..6717)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6817..6839
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6877..6895)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6963..7067
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7068..7136,7137..7265,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccat cgaattatca
     1561 actatgtata ataaagttgc ggcccattag gtcagacaaa aatttctgat ctttaaacct
     1621 tattgattaa aactaatttt tgcccgactt ttttgacaca tgcactagac gcttgtactc
     1681 aaaatttgga aaccttgctc aaatccacta ctcagatatg acagtagcat cgataattga
     1741 accattatcc tgaaatcccc tttcctgtcc cggttcattt tccaattttt tcaacggtgg
     1801 aagaaggggc aaattttata ccaaaacgtc tccgtttatg acccaccccc tatgcaaaat
     1861 aaacggctct gggagaaata gtacagaaaa cgcggagcct gcttgattcc ttctccgagg
     1921 cgacaaaaaa cgcatcattc gtattgtaag atgcatgtgt ggctggcctc ttttgaattt
     1981 tactggtttt ttatatttct actaatattt cgaaaaattt tgtaagtaaa tcattgatta
     2041 cgtttacaaa aatttagaaa catttcaaaa tgattacttc taaattaatt atatttacag
     2101 gttagttcga gttacttatg accttccaat ccgccatatc cgccaaggac gctccaatta
     2161 attttttaga attatttttt aacacatgta ttatttttta acaataaatt attcatgaaa
     2221 tcatcaaatg tatatgttct actataatat ttaggtcaaa ctatagaaaa gaaatccttg
     2281 tttttggaac aaaggagttc agatcctgtg catttgatct tggatccaca tctttggtcc
     2341 atgggaaagc ttggcactga tcaagaagga ttcttcaaat atctaaaatt gccggctgcc
     2401 cgtagaattc catcagtttt ccagagtttt agtgaaaatt tgcatcccgt tagaaacaat
     2461 ctacacaacg gtaaaaagga cccattattt ttaccgtacc gttttccttt atggccctct
     2521 atgcaaaaaa ttatagcgtg gaggggcagt acagaataat aaagtgtgag aaaaaaaact
     2581 gctctgcgag aaatagtaca gcaaacgcgg agcatgcttg attctatagc cactttgtac
     2641 aagaaagctg ggtcacgggc aggaaacagc tatgaccatg attacgccaa gctacgtaat
     2701 acgactcact agtgggcaga tcttcgaatg catcgcgcgc accgtacgtc tcgaggaatt
     2761 cctgcaggat atctggatcc acgaagcttc ccatggtgac gtcaccggtt ctagatacct
     2821 aggtgagctc tggtaccctc tagtcaaggc cttaagtgag tcgtattacg gactggccgt
     2881 cgttttacac ccgtgcagtt gaaattgaaa atgagttaaa gttggagttt ttattgaaaa
     2941 cagatttccg tgtgattagt gtttttagcg agtgtgacag gacagcgaaa aaatatagaa
     3001 acaagggggg aactgaaaag cttaggaatg cattgaacat gagaagggga aggggaagga
     3061 acaaactaga caggaattat tggaatttaa tcacatttgg agtttttttt ctattcgaca
     3121 gaataattat ccagaacatt tttgtattaa atatttatgc atcatatgag tagtcggctt
     3181 tgttgtgcat gacgagtttg ttatcgacga aatagaagct gtcagaacga gtctcgtttg
     3241 gattgttgat catgtcgtcc actgaaaaag agattagtct tttgaattgt actttttaga
     3301 ataatgactc actgagttgt tgagagagtt gagggaactc atagatatgt tcacagttgt
     3361 ttcgtgaatt cggaatacag aatccgaatt caaagtcaaa acacttcaga aggcgatctt
     3421 tgaagaagtg acgttcgatc attcggaaat gatggattgg gatgtctcct actttgaatt
     3481 ccacagttgc tccgaccgtt ttgagtttca aaaagtttgg agcaaatcta taacgcacgt
     3541 atcttgccga ctcctgtggc gactcatcat tctcctgatc gttctccggt ttggcgatct
     3601 cgaaaagcac ttgctcagtg tccagatcac ggatttggaa cttggtgaac tcgatgttat
     3661 agatgttcgc agatggggag cataagaatc ctaaatttat gttttaaact gaaatccaaa
     3721 gggagcaaga taccttgagt gattcccgga agtgctaaaa cgtcgttcgg agtgatttga
     3781 gctttcttcg caagctccga ttccgttgtg attccttgtt cggtgcttgg tggtggccgt
     3841 ggcatctgga aatatggaaa agttcaacaa aaagaaaaga gaaaagaatg aaatcggata
     3901 tcaagagtta gttgagcggt ttctctagtt ttctgagtct cacctgcgac gggaaggtcg
     3961 ccgagccggg tggaatcgat cgttgttgct cggctttcat atcggtttgg ttggaagcgg
     4021 ctgaaaacgg aaagaagtgg aagaaggaaa agagtgtggt gtgacaggaa aatggtaatt
     4081 agagggtgcc aaataaccag ctatattttg tttttttttg aaaacatttt taaaaagaaa
     4141 aatacgataa tgatatcaga tggatttccg gaaaactggt atgaaaaatt tcaacctttt
     4201 tgagtacatg taatcaaaat acactttgta aattatcatt tttattgaaa ctccaccatt
     4261 tttctattta taacgctaat aatttgaaaa agaaacctat tgcgaaccgc ggggtgaatc
     4321 ccaaaaacga atgcgttttg gtggagtgat tgattcgaat cgaagaagaa aaagaagaag
     4381 acgtggaata gagagctcac tcttaaccga gcagcacaca ccgacagaaa aaaaaatgaa
     4441 atgaatgagg gtcttcttct tcttcttctt cgaatgattg acagaaatgg gaaaaagagg
     4501 aagattgaga agggaaaaag gaaggagaaa agaagcagaa gaagacgtca gagaggagag
     4561 gaacgagcgg aaaagcagcg ggcgcaagtc atagaagtag cagagctggg gagaagaaga
     4621 cactatccaa gaaaggaatg acgagagagt atgcaaaggg gtatagggtg cagacagaat
     4681 aggaacagaa taacagatga tgagccaaga agagttgaaa agggcgatga atttgtcatg
     4741 taacttaatt tgggtcaatt tgagcatgat gaattgaaat catcccttgt tgggagttaa
     4801 taaccggttt gttatcagaa accctgtaat agaagggcgc cctaactttg agccaattca
     4861 tcccggtttc tgtcaaatat atcaaaaagt ggtcaactga caaattgttt ttgatattat
     4921 aataaacatt ttatccgtta acaattttcg aatacttttt acaaggactt ggataaattg
     4981 gctcaagcct gcttttttgt acaaacttgc aattcaaaaa cgcagaactc gtactgttgg
     5041 atgcctgtgt agcggtcctc tattgatttt tgctattttt tgataatttt actgtaaatt
     5101 atgagttatt ccaattaaca ttcctagcta aatgtaagtt agcgaccaat tttttagcaa
     5161 ccccatttta tgacttttca gaatatcgcc tactgggtca acgacacttg tttggaacta
     5221 agggtcttcc caattttttt taaatcttca taggcttgac agaatcaggg ttcttcaagc
     5281 agctttgaga gggaatcaat tgcaacattt gatgttaaat ttatattttt tatagttctc
     5341 tggtgaaatg gggaccaccg caaagcccct tttatgtttt taacttgaat aaaatcttgt
     5401 attaaattgt gatttataga tctgtatggg taatttgaag gaaaagaaag ggagctccca
     5461 aaagtgcctt acagatccag gcccaataaa tgtaaactat tgggcagtag tatggcaaaa
     5521 atcgtgcgga atttgaaaaa cctagaatta aaataaatat tcaacacaaa tgttcgagat
     5581 attttaagtt cggagtgtat atctagaaca tattgtacaa gtaggtctac gggatcgtct
     5641 acgggaaaat ttgatttcct cgaagatgta tttaatgaat tctctaatgt tgcaggtctt
     5701 tgtatttgac agaatgttgg tttttgaaac ttattcgcca taattgcttg gctactacat
     5761 gtctcatgcc attgacacta tcctgtactc gaaatttgtt aagtaactac ccaccgtcca
     5821 cttatgacga gagggtcaat taaggtcact ccacaaaagt aaatttgaaa acacatgaga
     5881 aaaagtgatc tgaattccct tgaagtggct aaaacaaagc agatgaggga tacgacgtat
     5941 gcgactactg agagataagg tcgaaacttg ataaggatgc tgcttacctg cttagcaagt
     6001 ttcttcgcac aggagaaagt gacaagctaa actatcaaat tcatctaaaa cctcaaaaaa
     6061 ttattttgga tgaaataatg ttttattttg agcaacgcat aaggcaaaga ataaattaaa
     6121 ggcgatccgc ttagcggcga gttccgtcct cgaagaagat tcctccatcg atggcgcagt
     6181 actttggaca gattccacgc tctcctgggg ttcctggtgg tccgactggt cctggctgtc
     6241 ctgggtttcc gtcggctcct ggcttgccgt ctggtcctgg tggtcccttt ggtccaggtt
     6301 gtcctgaaag atgtggtgat tagatatgga ttgaaaaact atgaaatgat tacctggggt
     6361 tccgtcgttt cctggttgtc cggatggtcc tggtggtccg actggtcctg gctctccggt
     6421 ggatcctggc tctccctttt ggactggctc gcagacagct ggaagtccct cagcaacttt
     6481 tctatacaaa gttgatagct tggcggatcc ctggcgttac ccaacttaat cgccttgcag
     6541 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6601 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     6661 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     6721 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     6781 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     6841 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     6901 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     6961 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7021 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7081 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7141 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7201 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7261 caatg
//
