LOCUS       sequence-421691-         492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm,
            to allow TEV protease to remove SpyTag003DA and thereby uncage 
            SpyCatcher003 reactivity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Driscoll CL, Keeble AH, Howarth MR
  TITLE     SpyMask enables combinatorial assembly of bispecific binders.
  JOURNAL   Nat Commun. 2024 Mar 16;15(1):2403. doi: 10.1038/s41467-024-46599-9.
  PUBMED    38493197
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-03-16"; volume: "15(1):2403. doi"; pages: " 
            10.1038/s41467-024-46599-9"
COMMENT     Sequence Label: pDEST14-SpyCatcher003-TEVs-SpyTag003DA
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             409..429
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             442..489
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVAAYKRYK"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgaca tcccaacgac cggcgccatg
       61 gtaaccacct tatcaggttt atcaggtgag caaggtccgt ccggtgatat gacaactgaa
      121 gaagatagtg ctacccatat taaattctca aaacgtgatg aggacggccg tgagttagct
      181 ggtgcaacta tggagttgcg tgattcatct ggtaaaacta ttagtacatg gatttcagat
      241 ggacatgtga aggatttcta cctgtatcca ggaaaatata catttgtcga aaccgcagca
      301 ccagacggtt atgaggtagc aactccaatt gaatttacag ttaatgagga cggtcaggtt
      361 actgtagatg gtgaagcaac tgaaggtgac gctggatcca gtggtagcga aaacctgtat
      421 tttcagggcg gtggtagcgg ccgtggcgtt cctcatattg ttatggtggc cgcctacaaa
      481 cgctataaat aa
//
