LOCUS       sequence-421694-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher 
            H-lock is a variant of DoubleCatcher in which each SpyCatcher003 
            moiety is separated by an alpha helical linker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Driscoll CL, Keeble AH, Howarth MR
  TITLE     SpyMask enables combinatorial assembly of bispecific binders.
  JOURNAL   Nat Commun. 2024 Mar 16;15(1):2403. doi: 10.1038/s41467-024-46599-9.
  PUBMED    38493197
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-03-16"; volume: "15(1):2403. doi"; pages: " 
            10.1038/s41467-024-46599-9"
COMMENT     Sequence Label: pDEST14-DoubleCatcher-H-Lock
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             82..420
                     /codon_start=1
                     /product="improved SpyCatcher variant that reacts with 
                     SpyTag003 at a rate approaching the diffusion limit (Keeble
                     et al., 2019)"
                     /label=SpyCatcher003
                     /translation="VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATME
                     LRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATPIEFTVNEDGQVTVDG
                     EATEGDAHT"
     CDS             883..903
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgaca tccccacaac tgagaacctt
       61 tatttccaag gtggggcgat ggttactact ctgagtggtc tgagcggcga acagggtccg
      121 tcgggtgaca tgacgacaga ggaagacagt gccacgcata ttaaattttc aaaacgtgat
      181 gaggacggtc gcgagttagc aggggcaact atggaattgc gtgactcctc cggcaaaacc
      241 atttctacat ggattagtga tgggcacgta aaggattttt atctgtaccc cggaaagtat
      301 acctttgtcg agacagcggc tcctgacggt tatgaagtgg caaccccgat cgaatttacg
      361 gtgaatgagg acgggcaggt gacggtagat ggggaagcca cagagggcga tgctcatacc
      421 gggtctcccg ccaacctgaa ggctttggag gcccagaagc agaaggagca gagacaggcc
      481 gccgaggagc tggccaacgc caagaaactg aaagagcagc tggagaaggg atccgtaact
      541 actttaagtg ggttgagcgg agaacaaggg cctagtggag atatgacaac cgaggaggac
      601 tccgccaccc atattaaatt tagtaaacgc gatgaagacg gtcgcgagct tgcgggtgct
      661 actatggagc tgcgcgatag tagcggcaag accatctcga cctggatttc agatggacat
      721 gtcaaagact tttacctgta ccctggcaaa tatacctttg tggaaacggc agctccggat
      781 ggatatgagg ttgcgacgcc gatcgagttc acggtcaacg aagatggaca agttacggtg
      841 gacggtgagg caactgaagg agatgcaggt agttcaggct cagaaaactt gtattttcag
      901 ggcggaggat cggggcgcgg agtgccccac attgttatgg tggccgccta caaacgctat
      961 aaataa
//
