LOCUS       sequence-421705-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To
            be paired with 39S light chain to form the anti-HER2 39S Fab.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Driscoll CL, Keeble AH, Howarth MR
  TITLE     SpyMask enables combinatorial assembly of bispecific binders.
  JOURNAL   Nat Commun. 2024 Mar 16;15(1):2403. doi: 10.1038/s41467-024-46599-9.
  PUBMED    38493197
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-03-16"; volume: "15(1):2403. doi"; pages: " 
            10.1038/s41467-024-46599-9"
COMMENT     Sequence Label: pcDNA3.1-39S Heavy chain-SpyTag003
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             766..813
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
     CDS             817..834
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggggatcc ttcccagccc tgggatgcct gcgctgctct ccctcgtgag ccttctctcc
       61 gtgctgctga tgggttgcgt agctgaggtt caactggttg aaagcggtgg cgggttagtt
      121 aagcctggtg gatcacttcg cctgagctgt gcggcatcag gctttacctt ctcttcttac
      181 tcgatgtcat gggtccgtca ggcacctggg aaggggttag agtgggtctc aagcatctcc
      241 tcatcttcat cgtatattta ctatgctgac tcagttaaag ggcgctttac aattagccgt
      301 gacaatgcta aaaattcatt atatctgcaa atgaatagcc ttcgcgcaga agatactgcc
      361 gtgtactact gcgcacgtgg aggtgacgca tacaactact actatttcga ctattggggg
      421 caaggcacct tagtgactgt ttcatctgcc tccaccaagg gcccatcggt cttccccctg
      481 gcaccctcct ccaagagcac ctctgggggc acagcggccc tgggctgcct ggtcaaggac
      541 tacttccccg aaccggtgac ggtgtcgtgg aactcaggcg ccctgaccag cggcgtgcac
      601 accttcccgg ctgtcctaca gtcctcagga ctctactccc tcagcagcgt ggtgaccgtg
      661 ccctccagca gcttgggcac ccagacctac atctgcaacg tgaatcacaa gcccagcaac
      721 accaaggtgg acaagaaagt tgagcctaag tcttgcgggt ccgggcgtgg cgtgcctcac
      781 atcgtgatgg tggacgccta caagcgttac aaggggcatc accaccatca tcattgataa
//
