LOCUS       sequence-42171-e        8941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ355 - MCS(ttTi14024, X)
FEATURES             Location/Qualifiers
     source          1..8941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1559..1579
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4009..4033
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     4040..4056
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4040..4056
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4252..4269)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4252..4268)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6209..6220
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6366..6390)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8150..8170)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8374..8393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8493..8515
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8553..8571)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8639..8743
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8744..8812,8813..8941,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccat cgaattatca
     1561 actatgtata ataaagttgg gggatgaagt ttaaaggttg agtagcgcat gtggggaaac
     1621 tgttaaaaac cactcctttg gtcctaaaat attcaaatat cataataaaa attttcaaaa
     1681 aatttttgaa ttttttttat ttcctgtcaa aaaatgacaa ttactcagtt tttgccactc
     1741 ataattttgg aagacgacca aaaaaaacat ttttttctac attttttata ttttaatttt
     1801 gttttgatta cttgtattga aacattgtaa gggtcggaac atgcgacatt gctttggatt
     1861 tcctcaaaag ctcatatttt tcaaaatttg gcaatttgcc aaagctccga aattatgaaa
     1921 aaatctgaaa attttcggag tgttttgtta tgatatttgg ttgttttagg tcattataat
     1981 tgtacttaga ctaaatccct actggcgcta ctccaccttt aaaactgaaa aactttaaac
     2041 tcaaaattcg tagtttttag attatgtttt agtgttcaat tgagaaccat gtgagaaaat
     2101 ctttttcaaa aaatatcaaa aattcataga aagttaaata aaatgctggc tacgcactgg
     2161 caagtttata atacaatgtt gtaaaaaatg atgagcttct gtgatgagcc catttttcgc
     2221 aaatacgttc actttgaagg agttgttgta catacataca gaggtagatt tgaaacagta
     2281 gcttatatat ggtcttgttt aaatttttta atctacctag ttttgttaaa aagttagaga
     2341 aaaaaccgca ttccatgaaa ttaaattaat actgaagttt ctcaaaaagt ttgatgacac
     2401 aatttttaaa attgaaattc cttgtatgtt tctttattat ttggttttta atctaatttt
     2461 tacagattta gcgttaattg ataaaaaatt catcgcaatg gccgagtaga attgaaatct
     2521 tttttttaaa tttttctaac tttttaaatg attttttttc catttcacgc ttagttttat
     2581 tgagttttcc gttttctgaa aattagtttc aatgggtttt tgaaatgtga cgtcatctta
     2641 gaaaaaccgg agaaggtcat ttaaattttc agaaaaacag aaaactacaa aaaatgatgg
     2701 gtctgggaca tcagccatac gaaatttttt tcaaaatttt aaataaaatt taaaaaaata
     2761 ataaaacatt ttttagactt attttctata tataatataa ctttaaagta gggattaaaa
     2821 ttttcaaaca aatgtttttc ttccattatt tttgtaataa aaattgaaac atagacaagt
     2881 ccaacccaga gcgaggaaaa aaaataatga tcgggataaa aaacgttgtt tgctgatttg
     2941 ttggcggatg acacaacaca acggcaagtg ctcgatgagt atcgaaaata tgctgctgct
     3001 gggttgctcc aactactgct gaggttgtgc tgtgctactc ctgtgccaga agaaactgcg
     3061 ccgtcatcac tttacacacc attcttgctg ccgaagagaa atggcactgc tggatgaaga
     3121 agaggaagag aaggcgggtg cagaagagga gtagatgaaa agattcgttt ctcctacttt
     3181 ttcaatcacc aatcgataag caactagagt ctcaagtcca agagtcactt taatgatgat
     3241 ggcgaaacga cgcaaaaaca ggagaagcaa agttcagaag gaaatggggg ggatcaatca
     3301 aatgttactt ttgacctgag ttttttatac tgttcaatgt atttaaaagt gtgacatggg
     3361 ttggcacagt gataagtatt caagagagtt tcacaatatg aactcgtggt gtcagagtgt
     3421 ctcatttcgg tttgatctac aaatatctac aaaaaatgcg ggagttgaga cacagagttc
     3481 ttaactgatt taaatggcta aaaacgtgct gacgtctcat tttttgggca aaatttgtaa
     3541 gtcaaaccgt aacaacctga caccacgtgt atgaagtatt tcaaaaaaaa tgaatggaat
     3601 cttgaacaaa aacctttgaa aacttcggta aaattgtttt accctcaaaa ttcgtgaagg
     3661 gaaatattct aatcttctgt tttgtatcca aaaactaatc cgagttttta gattctttct
     3721 cctagccttc gttttttatc catagaggta ataaaaatgt tgcaagcttt tataacaaaa
     3781 aaaatattaa aaggtaaaat cattcttttc tatgtatact caacgtgcca gttgttgact
     3841 tatgacacat ttgaaattaa atgggtgcaa acagacaact tctacaaaat tttaaaatgt
     3901 agtgctcgtg aaaaagttat taaaatttaa gaaatacaaa gcaccatttt ttattctgat
     3961 tctgaagggt ttgaacgaat tcacaagttt cctgaaactc acaaacaaac cactttgtac
     4021 aagaaagctg ggtcacgggc aggaaacagc tatgaccatg attacgccaa gctacgtaat
     4081 acgactcact agtgggcaga tcttcgaatg catcgcgcgc accgtacgtc tcgaggaatt
     4141 cctgcaggat atctggatcc acgaagcttc ccatggtgac gtcaccggtt ctagatacct
     4201 aggtgagctc tggtaccctc tagtcaaggc cttaagtgag tcgtattacg gactggccgt
     4261 cgttttacac ccgtgcagtt gaaattgaaa atgagttaaa gttggagttt ttattgaaaa
     4321 cagatttccg tgtgattagt gtttttagcg agtgtgacag gacagcgaaa aaatatagaa
     4381 acaagggggg aactgaaaag cttaggaatg cattgaacat gagaagggga aggggaagga
     4441 acaaactaga caggaattat tggaatttaa tcacatttgg agtttttttt ctattcgaca
     4501 gaataattat ccagaacatt tttgtattaa atatttatgc atcatatgag tagtcggctt
     4561 tgttgtgcat gacgagtttg ttatcgacga aatagaagct gtcagaacga gtctcgtttg
     4621 gattgttgat catgtcgtcc actgaaaaag agattagtct tttgaattgt actttttaga
     4681 ataatgactc actgagttgt tgagagagtt gagggaactc atagatatgt tcacagttgt
     4741 ttcgtgaatt cggaatacag aatccgaatt caaagtcaaa acacttcaga aggcgatctt
     4801 tgaagaagtg acgttcgatc attcggaaat gatggattgg gatgtctcct actttgaatt
     4861 ccacagttgc tccgaccgtt ttgagtttca aaaagtttgg agcaaatcta taacgcacgt
     4921 atcttgccga ctcctgtggc gactcatcat tctcctgatc gttctccggt ttggcgatct
     4981 cgaaaagcac ttgctcagtg tccagatcac ggatttggaa cttggtgaac tcgatgttat
     5041 agatgttcgc agatggggag cataagaatc ctaaatttat gttttaaact gaaatccaaa
     5101 gggagcaaga taccttgagt gattcccgga agtgctaaaa cgtcgttcgg agtgatttga
     5161 gctttcttcg caagctccga ttccgttgtg attccttgtt cggtgcttgg tggtggccgt
     5221 ggcatctgga aatatggaaa agttcaacaa aaagaaaaga gaaaagaatg aaatcggata
     5281 tcaagagtta gttgagcggt ttctctagtt ttctgagtct cacctgcgac gggaaggtcg
     5341 ccgagccggg tggaatcgat cgttgttgct cggctttcat atcggtttgg ttggaagcgg
     5401 ctgaaaacgg aaagaagtgg aagaaggaaa agagtgtggt gtgacaggaa aatggtaatt
     5461 agagggtgcc aaataaccag ctatattttg tttttttttg aaaacatttt taaaaagaaa
     5521 aatacgataa tgatatcaga tggatttccg gaaaactggt atgaaaaatt tcaacctttt
     5581 tgagtacatg taatcaaaat acactttgta aattatcatt tttattgaaa ctccaccatt
     5641 tttctattta taacgctaat aatttgaaaa agaaacctat tgcgaaccgc ggggtgaatc
     5701 ccaaaaacga atgcgttttg gtggagtgat tgattcgaat cgaagaagaa aaagaagaag
     5761 acgtggaata gagagctcac tcttaaccga gcagcacaca ccgacagaaa aaaaaatgaa
     5821 atgaatgagg gtcttcttct tcttcttctt cgaatgattg acagaaatgg gaaaaagagg
     5881 aagattgaga agggaaaaag gaaggagaaa agaagcagaa gaagacgtca gagaggagag
     5941 gaacgagcgg aaaagcagcg ggcgcaagtc atagaagtag cagagctggg gagaagaaga
     6001 cactatccaa gaaaggaatg acgagagagt atgcaaaggg gtatagggtg cagacagaat
     6061 aggaacagaa taacagatga tgagccaaga agagttgaaa agggcgatga atttgtcatg
     6121 taacttaatt tgggtcaatt tgagcatgat gaattgaaat catcccttgt tgggagttaa
     6181 taaccggttt gttatcagaa accctgtaat agaagggcgc cctaactttg agccaattca
     6241 tcccggtttc tgtcaaatat atcaaaaagt ggtcaactga caaattgttt ttgatattat
     6301 aataaacatt ttatccgtta acaattttcg aatacttttt acaaggactt ggataaattg
     6361 gctcaagcct gcttttttgt acaaacttgt ataatgtaaa attgcaaaat cgttagtagc
     6421 cacactgaaa gagactacat aaaatgagaa acggatatgg gaaattttca acttttaagc
     6481 agtcgtcatg tacgtgttcg acatgttctt ttatgttccc gaaccattaa gtgtcagcaa
     6541 ctacgctgat caaatgaaac ctaaagtcac taaccgtcat caattgaaaa attagttttc
     6601 atttaagtaa taaactggct ggcgttcagt tgaacggatg gttccatcaa caagtatgca
     6661 atttcgacga gttctttggt atttgatatc gttataacaa aagtatgcca gaattgtagt
     6721 tctgcgttga caacgattct gacgagatct aaaattttaa atcggaaaca tttattaaat
     6781 ttttagtacc tactcaccag cgccaaaaac atgttacatt gctacttatt agagtttggc
     6841 atattgaggg tttatctgcg aagcttgagg atatggatta aaaaacttct atttgtttta
     6901 agtatcaatt gaaaaggttc ctttttttaa atgtacaagc gcagcaatac acatataata
     6961 attttcaaat aaagttctcg ttttagtaca tgctttaagt tttaaatggg ttcttttcaa
     7021 aagtgccaaa ttttgtcaag ttttcaaagt cttttttcaa gtttttttca atgtcttagc
     7081 aaccggaatt ttctaatatc ttttcttcat tgtggatttt ttaaccatta attgtttaag
     7141 gtatattttc cgtttatagg tgcaccaatt cactatcatt tgttcatctt tcgaaagaaa
     7201 acatatacct accaatcaat gaagaaaata aacgtaataa attaccaaaa tgtgattaca
     7261 aatgataatt attttcaaga ttgcttccga ccgctgcaaa ccaaacaatt tcacaaaaat
     7321 tagggatttt tccaagaata aacagacttt cctacatcta catttatagg gccaatgttt
     7381 tgcacaaaac taatactaca tttttccgtt ttaactttat tccactttca cgaataaata
     7441 caaattctac acaatacgtt tcaaaaatct ataatttagg ctttttcaga tcattcttct
     7501 caacgaggga aaacctagaa gagcttataa tctaagaaaa atctataaat atatttactt
     7561 cttataaata tgggcttaaa ataatcaaaa ctgggatttg tggtggaggg ggaattgggt
     7621 gggatgaaga aagaacctac aaatgcacta aaagagttta tacattgggt ggccataaag
     7681 aacatctaaa taaatatagg cagactgggg ggggggggtt attgtaagtg acaaagtatt
     7741 gctatagaag agtaggaaaa atgtaaagtt tcaaatcata gaaaaaataa aatcaataaa
     7801 ttattgaaca aagtgagttt ataaaacatg caaataaaat tattgggagg tgggggaaaa
     7861 aaagagaaat aggactcgat tggttgtgga aaattcgccg ctgacaaaga aaaacacagt
     7921 tggaacggga gagagaatgg agaggtggat gaattgacgc ctagttagcg ctgggaccaa
     7981 tgcaatcctc aacgagactt gctcaaaagg tggaagaact cgggatattg attaatttaa
     8041 aaattctcaa aaaaaaaaaa gaaatgcttg ataaaacggg ggcattgtac aattactatg
     8101 ggaacggtaa tagtatgaca agtgagctag gatggagatg taaaagcatc aacttttcta
     8161 tacaaagttg atagcttggc ggatccctgg cgttacccaa cttaatcgcc ttgcagcaca
     8221 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     8281 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     8341 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8401 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8461 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     8521 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     8581 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     8641 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8701 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8761 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8821 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8881 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8941 g
//
