LOCUS       sequence-421713-         444 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an 
            anti-HER2 nanobody..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Driscoll CL, Keeble AH, Howarth MR
  TITLE     SpyMask enables combinatorial assembly of bispecific binders.
  JOURNAL   Nat Commun. 2024 Mar 16;15(1):2403. doi: 10.1038/s41467-024-46599-9.
  PUBMED    38493197
REFERENCE   2  (bases 1 to 444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-03-16"; volume: "15(1):2403. doi"; pages: " 
            10.1038/s41467-024-46599-9"
COMMENT     Sequence Label: pET28a-nanoHER2-SpyTag003
FEATURES             Location/Qualifiers
     source          1..444
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             364..411
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
     CDS             421..438
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggaagttc aactggtgga atcgggcggt ggtctggttc aagcgggcgg ctcactgcgt
       61 ctgtcctgtg ctacctcggg catcacgttt atgcgttatg cactgggttg gtaccgtcag
      121 agcccgggta aacaacgtga aatggttgca agtattaact ccggcggtac cacgaattat
      181 gctgattcag tcaaaggccg ttttaccatc tcgcgcgaca acgcaaaaaa tacggtgtac
      241 ctgcagatga acagtctgaa accggaagat accgcggtct attactgcaa tgcccgctgg
      301 gtgaaaccgc aattcattga caacaattat tggggccagg gtacccaagt gacggttagc
      361 tctcgtggcg tgcctcatat cgtgatggtg gacgcctaca agcgttacaa gggttcaggt
      421 caccatcacc atcaccatta ataa
//
