LOCUS       sequence-42173-e       12077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12077)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 12077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ676 - MCS(ttTi4348, I, unc-18(+))
FEATURES             Location/Qualifiers
     source          1..12077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(705..1214)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(900..921)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1108..1127
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      1325..1913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1814..1833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1974..1995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2010..2015,2016..2033,2034..2040)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2048..2064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2053..2075
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2072..2088
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2072..2088
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3701..3717
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3725..3745
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    3745..3852
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     3769..3785
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3769..3785
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3819..3835)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3820..3836)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(3854..3874)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(11538..11554)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11547..11569)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        11775..11879
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11880..11948,11949..12077,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaccccg gttgataatc
      721 agaaaagccc caaaaacagg aagattgtat aagcaaatat ttaaattgta aacgttaata
      781 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
      841 aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagggttg agtgttgttc
      901 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
      961 ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt
     1021 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1081 ggggaaagcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     1141 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     1201 gctacagggc gcgtaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1441 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtaatgtg
     1981 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     2041 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgggtcacca
     2101 ggtatcgcgc tttaattagg gtgcaagttt tttataattt ttaatcccat taaaattcaa
     2161 agcagaaagg ttctgatgct ctacgcaaaa tatcaaaatc acaatttatg acaatattgt
     2221 tacaaaaatc caaaaaaaaa attgcagttc gaaatggtga ttgaagaaaa cggattacgt
     2281 gaagctactc gacgaatcat atcctatttg gaaaaatata ttcatgcact gcactatcat
     2341 cataaggatg atgagattta ataatttttg taatttatct tacaattagc attttattta
     2401 attttaaaaa aatttgaatg ggaacataaa gtcaaattcc tgagctgcat ggctaagggt
     2461 gccctcgaaa attttccgat tgttcgattt atttcagaga aattcggaaa atgcgaaatc
     2521 gaaaaaaagt gtagaaaatc aaaaaatgca aaaatttata ccaaaccgta aaaaattacc
     2581 aaaatttaaa gatattttac ggaaacttac gcttgagtta ttcaaacaca taactgatat
     2641 tataacatta atactatatt aatactagat taatatgtac tatagcaatt ttcaaaatat
     2701 tttcaattag caaaatgttg ccgatcattt ttctacattt acaattaaaa ttaaaatttg
     2761 ataaccctga tccggaaaaa gttacggcag ctgacatttg tctgattttt gactagaatc
     2821 gctaagtggc ccacaatttt taaactaggt gttttcacga aacaaagttt tctatttttt
     2881 tgcaattttt tatcctgtca caaaaggacc ataaaaattt tatacaaaag attaaacatg
     2941 aaactcaaat ttcaaaaatt agagtgtttc gtaaaaagta acgtacatat ggtgccaaaa
     3001 tggccaaata tcatagtaaa atttttcaaa acatttttga aaaaagtggc aattactgag
     3061 tttttgccac ttttttttag taaactatag ctctctcgca tactcgaatt cctacaaaaa
     3121 tttatatttg aaatataagt accataaaat ccggagaaaa acttccaaaa aaatggcaaa
     3181 aactaacatt cttccaattt tcaaaaaatc atataaaatt tggaattttt taaagaattt
     3241 ttatactatg atatttggac ctttaagctg cgtttttgta gttgacaatt taaaaaaaaa
     3301 ctccaaattg ctttgaaaac taccaaaatg agtaccctga taaccatatt gaaaaaccat
     3361 caaatgggta atgtctagaa aaatgaacga aaaattagag aaaataggaa atgagatatg
     3421 ttcttcttcc tattctttat cttctaagct tctcacacct tttctctcgt ctctccacga
     3481 tttacacact atttgcaatg aatctcactc tgatgagcgt atctatcaag tccttgtctc
     3541 ttcttttgac aaaacttcaa agaaatcgcc gacttgcgag gaggggggag cgaaaaataa
     3601 ttgattactg aaatacaaac actgatctta cttgcactta taatacgact cactagtggg
     3661 cagatcttcg aatgcatcgc gcgcaccgta cgtctcgagt gtaaaacgac ggccagtgaa
     3721 ttatcaacta tgtataataa agttgagctc caccgcggtg gcggccgctc tagaactagt
     3781 ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg
     3841 gggcccggta ccccaacttt tctatacaaa gttgatagct tggcgtaatc atggtcatag
     3901 ctgttcctgc ctaggtgagc tctggtaccc tctagtcaag gggggcgacc ctaagcttta
     3961 attgccaact gtgtcatcaa gaaaaacgta ttgtcagagc tttttttttt aattgtgaaa
     4021 tttttgaatt tcttttttga ttgttagaaa tagttttaaa agtcttgtat tgtggtgcta
     4081 cattatagat gcacaaacag taaatattga tctaaatttc tcaaaaagtc aaaaaaatat
     4141 ggttttttat ttggcgtaaa aaataaactt cgacggataa ttcttaaaaa tcaatatttt
     4201 ctgtagtatt ttattaaggt ggtcatgtaa gacaaagagt aagcctttga aaacttttaa
     4261 tttttatttt tatcgccatg aaggaaattg atgtgacatt ttggaatata gtcattattg
     4321 tgttttaatt cgtaaaataa ttatacaaat agttattccg tcgagttgat ctttattatg
     4381 cgagtgcaaa ttctattcaa aaaatggttg gaacaaatgg cgagaaacga aaggaaaatg
     4441 taccaggaaa atcgaggaat gtttttgaaa tgcacaactc ttttgactag acaaaaacac
     4501 aacgtcaaat cacaatcctt ggtttttgga attattgcaa cgaggaatcc tcattttcag
     4561 attaaaagca agataaaaaa caataaaagt cgtagcaaca cagaaacact agcctatgac
     4621 taaatttttt tcaaaccggg ggatacaatc attagataaa aataaatact atatctagcc
     4681 gagcacatta ttcaaatagt cttaaagcat aaaaatcaaa ttatttaaat ggtcagtgaa
     4741 gggtaataca tgtaactgga cgaagaaact tattaaaaac tatacatata cagttacatt
     4801 atttttggcc atgggaagag aaattaactg aaattgtttc gggtttgata gactgttttt
     4861 cagaagtttt gtgaggtaat atcaataaat aatgaaggtt aattgagcgc actagctatt
     4921 gttgaaggct tagaaaaatg acttagaggc aacaagctaa atgatgacaa agaataaatt
     4981 actggtggga gaataagaaa attcccaaac gtgtgtgggg cacgaaaaca atctatgaga
     5041 aaaatttact gtaatgaaat aatggtcagt gtttggaaaa tgggagggaa atctcaaata
     5101 gggccgtatc ttgttgtttc gtcaattgtc gattcttttc ggtaccccgc actctgtcat
     5161 atgtcacgcg gtttgttcag gtcacgcaag ttggtcagga acttatccgg agtgatgatt
     5221 ctgtcggacc caatgacgac ttcccacggt tttcgggcag cggtcacttc ataacaagct
     5281 cgcatttcgg agaaggtgac acctccaata atgtagataa tcagtcgtgg tccactgcga
     5341 tagttggatt gttgtcctcg ttccttgtgc cactgaccat atctagcaga tgcaggggct
     5401 ctgaaaatga aacagggttg aaatgagcaa tcatcagttt ttgctagatc agagagaagt
     5461 tttgggatga ctaatcaatc caattgttaa aattagatac ctgtatcctt ggttaacttg
     5521 tcgtccagca aggaatggga aatgttttgt gtccaaacgc tcgtcaatag catcttcaat
     5581 aatatccttg ataactggaa cccagcggga agattggtaa acttgctcgt gtggtctttc
     5641 ctttttggtt ggggtccacg tcttcttgcg gccggtctgt aaattgacac gtctatttac
     5701 atatgaaaat acaaatccga tttatgcgta ctgtttatgt tcaaaaaaaa aaacttacat
     5761 cggtaacaat attcaatccc aagtaagcag cgttggtgat tgtttcctta tcagccatgg
     5821 agatattagc gtgttgtaaa agtttattca agttttcatc agtaattcca ttcttggaaa
     5881 gaatgtacaa cagaatcaat ctgaggcggt cttcacaccg cacggctggg tcaatcaaaa
     5941 gtggcaccat caacttcatt gcatctctga ctcgttctcc ttctgcgtcg attccggtac
     6001 tcaaatcttg ttcaacctta cacaacttgt caactccttg ttgatactgt ttcatgcatt
     6061 cctcagcaag actgatgtga gtgctaaact tgttcaactc tttcttgtgc tgtggcattc
     6121 ttttgatgag catcgataga tccttgatcg atttggagtc catcgttccc ttgttgccct
     6181 tgctctcact gaactttttc aagttctttg tgacttcttg tgaaacaacg gcaatatgtt
     6241 tgtgcctcat ttcaacccat aaatcatcat tctcatcgag cagaacctgc aagaacaaat
     6301 tatcatccgt tatttgcgta cactataatt gcatgtttcc tggacaacaa catacctctt
     6361 tctccaagtt ttcatcgctt cctcccgttt cgtacttgta aacgtcattc tcaatgccca
     6421 aaaggtcgta gcacattgct tgaagagtaa gttcatggag aagtggagtg atggcatcgt
     6481 aacctctgtc gatgatgatc aactgactgc gtgccttgtc ggctccctca cccatagatg
     6541 gatcgtcagc cttgtaagcg tcgagttttt gctcaacaag gtggccgagc tcaacatttc
     6601 tctcaaagtc agcacggtac ctgaaaactt gattagtttt agaaaaactc atatgaaata
     6661 ttttatgttt tttctttcgt tacttacctc aatgatggat attctccaag tgttgcgcac
     6721 accgttgcga tttgttcagc aattctctca aggttcgatg tcagaccacc ttgtttttgg
     6781 gcgttgtagt ataagaaaaa tgtatcagga gagtccaaat tgaaaaccta aaaaagcaaa
     6841 ctatattttt gatttatttg gaacaaatct cacctggctt tcgtatggag tgaaagctat
     6901 gttaatctcc ttcagagttt tgatgaaacg agcagctgca cttttggata atgttgagaa
     6961 gagctgatcc gaacacgctt cggtgaaaaa gacatgagcg catttgtaca aatttctggc
     7021 gcaataatct tgaatcagct tgtctatctg caaaacatct tggtttagac aagcgacttt
     7081 tggagattaa aactaaccga ttcagcagtt ggagcaatca gataaatggc ttcaagtgtt
     7141 ggaaggggct ctctcctttt gtttagatct tccacaattg taattccttc tagaaaatag
     7201 agtgattaga gaaggaatta aaaatatcaa gtgtaattac cttccataat attgtgcatt
     7261 ttgcagcagg atgacaacat ccgcatggct agggtgtcaa cgatgagaac attccacgcg
     7321 ctacgcccgt ctcccttctt cagtggacgg attacatcgt tgagcagttc tttaaaagaa
     7381 aagttttagt aataattttc aaagtgtaat ttgttgtttt ccgtctaact tactgtgccc
     7441 aacgatttgt ttgagtgaca tcactcaaaa atcctcgtcg atgcactcac aattaacctg
     7501 caaccgaaac aaattgaatt aatcagaaga cgcaaaaaag gggagcgaag aacatgagac
     7561 ggaaagaaat aaaagcatga atagaatatg gggagtgttg cgaggtttgg ggccacccac
     7621 tataagacca acttgtttct caggtcagag cgtacaactt catggaggtc ttgagcacac
     7681 ttcatgattt tttcttctta cttcgcatgt gcatgaagac tacaaacggc agcaattcca
     7741 aagatggatg cacagaagag ataggaagaa atatgtatgt gtgtgtgtgt cgcgagagaa
     7801 gagcaatttc ggacttgtca ctgacgagta aagcaaaaat caataacgtg taagcaaacg
     7861 agggagagag tgtctgaatc tggtgctcag catgacacag agagaggaaa agagagaaga
     7921 attggcaaca cgaagaggaa gagcatgcca aataagaatg tttaacattt tgcattgatt
     7981 ttttctattt ttaagaaagt tattataaat aaaacgaata tttagaaaac ttattagttt
     8041 tctttgagta aatttaatta gttttttttc ttcaaagacc acataaccaa tttttctgaa
     8101 aaagttatgt ttagattttc caatgataaa ataccttgtt gacaaaattt aaaaataaca
     8161 attgatatca tcaacaagga acagtcgata catagacaca ttagggatta ggaccgtaga
     8221 caccacgggg actgtactgc gtactatgcg ctggaaatga cgcgcaatgg agtgcaatag
     8281 agaccagcat ataactattt caatgaaaat gggagattac ttatttaata atcggtattt
     8341 aggttttata atctcacatt ttaaaaaata aactaatttt tccatcaaat tttccatatt
     8401 aataatacgt gatacacgtg ttcctcttca agtttaagtg gttttccaag aataaaaatg
     8461 caaaaggatt tcgaacattt ccatttctga taaattaaaa aggtgttatg attcacaaca
     8521 aggtttaata aataacaaaa tacgtgcgta ctttgaaaac agttatatat atctaatttt
     8581 taaaattaaa gttcatgtat tttgtcgttt aatttttgac actatatgag cgggtttaaa
     8641 cagttttcct actgaaggta ctccgttttt aatttatata acaagaagag ctacaaaaca
     8701 ctttgtggtt tgtttcaagc agagaaaatg tgatcacaca tatgtactca aagaaaaaat
     8761 cacaatttta gtctctcttg atgactcagt tgactaaagg caaaaaagtc agcctcgttt
     8821 gcacaacaac gattgtgaaa tatattccgt tgcctttctt ttttgccagt tgcggaacat
     8881 tttcatatgt gtttgcgccg ccgaaaatcg tccatcattc gaaatatgga aagcgaaacg
     8941 atgtgtcttt gtatcttcaa aacggcattt ctcaattgaa gctcaatggc tcattgacat
     9001 tcgaagtgaa gacacagtgt agggtcacgg cgctaaaatg cgtgttttcc cattgtttta
     9061 atggggaaaa aggacaataa gagtagaaaa cggcaattcg tcaaaatgat tgattatatt
     9121 catcaattag tttgcaggaa acaacatgca gaaatgtgga aaagatttca gtgaaagttt
     9181 caattagttt ggaaaatcac taactgttga atatgaataa acaaatggag aaaaaacagg
     9241 gatgttaaaa tttttatcaa aagtcactcc tcaaaaaggt attccgttgc ggggaatcga
     9301 accccggcct tatgggtgag agccataaat cctaaccact agaccacaac ggagatgggt
     9361 gagcaatggt cgctgcttaa tgataagagt acagaaaact ttgtgactgt gaacaatgtg
     9421 tagaaaattt tatcagtcag aaagcagcac gcgaagaaat tgagaaaaat gatggttgcc
     9481 tttccggcaa tctctttggc aacagttcca gaatttcagc tcttatttta tatcttttaa
     9541 tcagttttca gactatgatc aaaactttgt tggatgttaa aaatattgga tgactcgttt
     9601 ctgatattaa aaattcaaac cagctgttta tcatcttgtt tctcacttcc ttcaactgct
     9661 cgtttttgct gttcgtctat gattccgcat cttttgctgt tgtaatgtgt gtattacacc
     9721 gcttccgatg agcagatagg agggccctaa ctttgagcca attcatcccg gtttctgtca
     9781 aatatatcaa aaagtggtca actgacaaat tgtttttgat attataataa acattttatc
     9841 cgttaacaat tttcgaatac tttttacaag gacttggata aattggctca aagacgaagt
     9901 gagtttgcta ccatatttca tctttatttt ttaattcgat tttctgatgg caatgactaa
     9961 ccgattttcg ggataattga gatgagggga ggtattttgg gaattgacag ggaggtattt
    10021 ctgcagattt tgttatcaga gtgtactgta gctctaaaac caatacttga cataatactt
    10081 gaatctatac agttgataag tttttttgca gtttcaagtt gaataaagtt atagtggccg
    10141 acatcttaga ggtctaaaaa tgagattttg gtcaaatttg agctagtcca attatacaac
    10201 taatcgcagt tcttgcgccc gacactttac gggatttcaa ttgtatatgt tttgtttcaa
    10261 ggtttcatct gttcagacaa tgcgaagcat tttgttggca ggtttacaaa gttttagcgg
    10321 ccgacacctc gcgggtttct aagataagat ttttgcgaag tcttcagcca tctgtaagct
    10381 acactagcgg tcgacatctc gcgggttaaa tctagctttt gaaaaactat ccaaacacta
    10441 gaaatattta aattttaatt ttaatttttg aaaaaaaatt ccattttaaa ttccggtttt
    10501 caaaaaaaaa caatagatca aaaaatttta acgtattttt ttgcaatttt ttcaaaatgc
    10561 ataactttca gaatatttct cacctcttcg catggtgaaa accgtcaaga gttttttttt
    10621 caaaataaat ggaaaaatcg aaaattttag tttatcaatt tttcgtatat tcgagaaaaa
    10681 tcttaaaatt ctggtcattt ttcttcttct tagaaaaata ttgtatgttt tctttctcat
    10741 taccataaac aaaatttgat aaaatattga attttgctgc tatttttctt aaaatatttt
    10801 tgtcgatttt cggttttcat aaatgtctaa aattcgttgc gtaactttat tgaattatca
    10861 gcaaaacaaa caaataaaaa acgaaaaata tccattttaa aaaattagat aaatgaatca
    10921 acacgtccct gaatgtaggg acgaatgtgc ggaagagact caaaagtctg gcaaaaagtt
    10981 cggagcctct cgaaatgagc cagatagaag ctatcgtagc actgctgaca ccgagttaga
    11041 aactcgacga cagcgatgtc agcccatgaa tatcgatctc caatcatgaa cttttgctcg
    11101 ccgagcaact cttcatattc tctgagcaat gggaagagtg actcctcttt gaatacattc
    11161 cagcattctt tctgaaaagg aagtattgaa ttttatgagg ctatctacct gcctgactac
    11221 cgcctatttc catgcgcagt aggcacatgt gtagacatag gcaggcatta tcgcgcctcc
    11281 cttcaaacta cctactaggt actaggtagt tcgaaaggag acgcgataat gcctgcctat
    11341 acctacacac gttcctactg cacatggaaa taggcggcag gcaggcaggt ggatagcttc
    11401 aaggtagtgc ctaccctcgc gtcatagttc ttcgtcagtg ttgctctcaa aattgggttc
    11461 atcgctgtgt tgagctcctc gagtttttga atcactttat caacttcttc ctgatcccca
    11521 cttggatcga ctgatcaact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    11581 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    11641 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttc
    11701 gcttggtaat aaagcccgct tcggcgggct tttttttgtt aactacgtca ggtggcactt
    11761 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    11821 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    11881 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    11941 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    12001 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    12061 aagaacgttc tccaatg
//
