LOCUS       sequence-421742-        8555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter designed on shortlist of Housekeeping-like 
            signature and transcription factor genes from Tabula Sapiens and 
            Tabula Muris databases..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Company C, Schmitt MJ, Dramaretska Y, Serresi M, Kertalli S, Jiang 
            B, Yin JA, Aguzzi A, Barozzi I, Gargiulo G
  TITLE     Logical design of synthetic cis-regulatory DNA for genetic tracing 
            of cell identities and state changes.
  JOURNAL   Nat Commun. 2024 Feb 5;15(1):897. doi: 10.1038/s41467-024-45069-6.
  PUBMED    38316783
REFERENCE   2  (bases 1 to 8555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-02-5"; volume: "15(1):897. doi"; pages: " 
            10.1038/s41467-024-45069-6"
COMMENT     Sequence Label: pLV-HKGT1_short_mCherry2-NLS-PEST_Puro
FEATURES             Location/Qualifiers
     source          1..8555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2264..2288
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2289..2298
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2440..2458)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             3021..3041
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3045..3065
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3069..3089
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3228..3252)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3270..3858
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3323..3343)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3741..3752)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3889..4388
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3924..3947)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4321..4340
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4405..5004
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4405..4424)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4901..4921
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5076..5309
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5381..5502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5418..5437)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5472..5491
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5542..5677
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5697..5716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5698..5716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5726..5743)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5726..5742)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5735..5757)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5884..6339
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5971..5990)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6181..6202
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6365..6469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6470..6538,6539..7330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6688..6707)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7501..8089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7990..8009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8243..8260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8377..8398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8413..8418,8419..8436,8437..8443)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8451..8467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8456..8478
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8475..8491
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8475..8491
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8510..8530
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8512..8530
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgtc tagagcgcga ctttttttcc
     1981 cggcggcccc gcggctctgc ccctcgtgcg gtcgcgttcc cggcggcgcg gggggcgtgg
     2041 cctgggcgca gcatctataa aggcgggcgg cggcagaggc gccattttgc gaacggcgag
     2101 cagcggcggc ggcgaagcgc ccgccggggg cggggagggg gcggggccat ccgcgtgagg
     2161 cgaccctgtt ggtccggagg ggcggggcga ggaggaggac ccgcttgggc ggttcggctg
     2221 cccacagtaa ccgctgggtg gacctggcca gcgctccgaa ccttcaagtt tgtacaaaaa
     2281 agcaggctgc caccatggtg agcaagggcg aggaggacaa catggccatc atcaaggagt
     2341 tcatgaggtt caaggtgcac atggagggca gcgtgaacgg ccacgagttc gagatcgagg
     2401 gcgagggcga gggcaggccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     2461 gcggccccct gcccttcgcc tgggacatcc tgagccccca gttcatgtac ggcagcaagg
     2521 cctacgtgaa gcaccccgcc gacatccccg actacctgaa gctgagcttc cccgagggct
     2581 tcaactggga gagggtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggaca
     2641 gcagcctgca ggacggcgag ttcatctaca aggtgaagct gaggggcacc aacttcccca
     2701 gcgacggccc cgtgatgcag tgcaggacca tgggctggga ggccagcacc gagaggatgt
     2761 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     2821 gccactacga cgccgaggtg aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     2881 gcgcctacaa cgtggacatc aagctggaca tcctgagcca caacgaggac tacaccatcg
     2941 tggagcagta cgagagggcc gagggcaggc acagcaccgg cggcatggac gagctgtaca
     3001 agctcagatc tcgagctgat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa
     3061 aggtagatcc aaaaaagaag agaaaggtag gatccaccgg atctagccat ggcttcccgc
     3121 cggaggtgga ggagcaggat gatggcacgc tgcccatgtc ttgtgcccag gagagcggga
     3181 tggaccgtca ccctgcagcc tgtgcttctg ctaggatcaa tgtgtagacc cagctttctt
     3241 gtacaaagtg gtgataatcg aattccgata atcaacctct ggattacaaa atttgtgaaa
     3301 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3361 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3421 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3481 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3541 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3601 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3661 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3721 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3781 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3841 tttgggccgc ctccccgcat cgggaattcc cgcggttcga attctaccgg gtaggggagg
     3901 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     3961 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     4021 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     4081 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     4141 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     4201 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     4261 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     4321 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     4381 gcctttcgac ctcacgtggc caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4441 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4501 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4561 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4621 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4681 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4741 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     4801 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     4861 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     4921 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     4981 atgacccgca agcccggtgc ctgaggtacc tttaagacca atgacttaca aggcagctgt
     5041 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5101 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5161 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5221 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5281 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     5341 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     5401 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5461 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     5521 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5581 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5641 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     5701 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5761 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5821 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5881 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5941 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6001 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6061 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6121 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6181 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6241 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6301 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     6361 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6481 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6541 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6601 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6661 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6721 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6781 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6841 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6901 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6961 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7021 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7081 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7141 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7201 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7261 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7321 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7381 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7441 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7561 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7621 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7801 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7861 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaaga
     7921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8041 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8101 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8161 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8221 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8281 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8341 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8401 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8461 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     8521 tcactaaagg gaacaaaagc tggagctgca agctt
//
