LOCUS       sequence-421748-        8711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter designed on shortlist of Housekeeping-like 
            signature and transcription factor genes from Tabula Sapiens and 
            Tabula Muris databases..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Company C, Schmitt MJ, Dramaretska Y, Serresi M, Kertalli S, Jiang 
            B, Yin JA, Aguzzi A, Barozzi I, Gargiulo G
  TITLE     Logical design of synthetic cis-regulatory DNA for genetic tracing 
            of cell identities and state changes.
  JOURNAL   Nat Commun. 2024 Feb 5;15(1):897. doi: 10.1038/s41467-024-45069-6.
  PUBMED    38316783
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-02-5"; volume: "15(1):897. doi"; pages: " 
            10.1038/s41467-024-45069-6"
COMMENT     Sequence Label: pLV-HKGT2_mCherry2-NLS-PEST_Puro
FEATURES             Location/Qualifiers
     source          1..8711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2420..2444
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2445..2454
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2596..2614)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             3177..3197
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3201..3221
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3225..3245
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3384..3408)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3426..4014
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3479..3499)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3897..3908)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4045..4544
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4080..4103)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4477..4496
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4561..5160
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4561..4580)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5057..5077
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5232..5465
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5537..5658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5574..5593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5628..5647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5698..5833
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5853..5872)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5854..5872)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5882..5899)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5882..5898)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5891..5913)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6040..6495
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6127..6146)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6337..6358
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6521..6625
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6626..6694,6695..7486)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6844..6863)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7657..8245
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8146..8165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8399..8416
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8533..8554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8569..8574,8575..8592,8593..8599)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8607..8623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8612..8634
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8631..8647
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8631..8647
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8666..8686
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8668..8686
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgtc tagaactcgc ccagtttggc
     1981 gcccaccagg cagtggggaa ggggaagggg aaggggaaga gggtgagggt ggcagggtgg
     2041 ggacagggaa tgggaagcag tggtggggat gtgggtggtg gaccaggggc gagggcaggg
     2101 gacaatgtct gtggtctaga cgcgccgttt gccttttatg gcggactcgt gggccgcgga
     2161 cgtggccggc tcagggccca ccctgcctct gagccacagg ggccgcgggc cgggggcggg
     2221 agggggcgcg ctcccccgag ggacccccgg gctccccgct gcccggctcc aagcgcccgc
     2281 cgggggcggg gagggggcgg ggccatccgc gtgaggcgac cctgttggtc cggaggggcg
     2341 gggcgaggag gaggacccgc ttgggcggtt cggctgccca cagtaaccgc tgggtggacc
     2401 tggccagcgc tccgaacctt caagtttgta caaaaaagca ggctgccacc atggtgagca
     2461 agggcgagga ggacaacatg gccatcatca aggagttcat gaggttcaag gtgcacatgg
     2521 agggcagcgt gaacggccac gagttcgaga tcgagggcga gggcgagggc aggccctacg
     2581 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     2641 acatcctgag cccccagttc atgtacggca gcaaggccta cgtgaagcac cccgccgaca
     2701 tccccgacta cctgaagctg agcttccccg agggcttcaa ctgggagagg gtgatgaact
     2761 tcgaggacgg cggcgtggtg accgtgaccc aggacagcag cctgcaggac ggcgagttca
     2821 tctacaaggt gaagctgagg ggcaccaact tccccagcga cggccccgtg atgcagtgca
     2881 ggaccatggg ctgggaggcc agcaccgaga ggatgtaccc cgaggacggc gccctgaagg
     2941 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgcc gaggtgaaga
     3001 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtg gacatcaagc
     3061 tggacatcct gagccacaac gaggactaca ccatcgtgga gcagtacgag agggccgagg
     3121 gcaggcacag caccggcggc atggacgagc tgtacaagct cagatctcga gctgatccaa
     3181 aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa
     3241 aggtaggatc caccggatct agccatggct tcccgccgga ggtggaggag caggatgatg
     3301 gcacgctgcc catgtcttgt gcccaggaga gcgggatgga ccgtcaccct gcagcctgtg
     3361 cttctgctag gatcaatgtg tagacccagc tttcttgtac aaagtggtga taatcgaatt
     3421 ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3481 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3541 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3601 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3661 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3721 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3781 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     3841 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3901 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3961 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcggg
     4021 aattcccgcg gttcgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     4081 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     4141 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     4201 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     4261 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     4321 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     4381 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     4441 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     4501 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctca cgtggccacc
     4561 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     4621 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     4681 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4741 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4801 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     4861 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     4921 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     4981 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5041 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5101 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     5161 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5221 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     5281 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5341 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5401 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5461 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     5521 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5581 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5641 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     5701 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5761 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5821 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     5881 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     5941 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6001 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     6061 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6121 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6181 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6241 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6301 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6361 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6421 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6481 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6541 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6601 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6661 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6721 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6781 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6841 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6901 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6961 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7021 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7081 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7141 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7201 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7261 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7321 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7381 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7441 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7501 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7561 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7621 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7681 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7741 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7801 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7861 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7921 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7981 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8041 gcgtgagcta tgagaaagcg ccacgcttcc cgaagagaga aaggcggaca ggtatccggt
     8101 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8161 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8221 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8281 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8341 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8401 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     8461 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     8521 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     8581 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     8641 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     8701 gctgcaagct t
//
