LOCUS       sequence-42177-e        5020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ421 - Pmyo-2::GFP::H2B (pharynx)
FEATURES             Location/Qualifiers
     source          1..5020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1559..1579
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(1583..1906)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    1911..1935
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2396..2416)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     3164..3192
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(3225..3249)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4229..4249)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4453..4472)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4572..4594
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4632..4650)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4718..4822
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4823..4891,4892..5020,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccat cgaattatca
     1561 actatgtata ataaagttgt gagacttttt tcttggcggc acaataaagt ttcctctttt
     1621 tgttgggtca ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt tctcaacctt
     1681 tcgggtttaa agcacttcaa aatggaattg aagagagatt gtgaaagtac atcagacaga
     1741 atcacatttt gatagcattc acttcactca gatgcaaact tttcagtgac tataaaaagt
     1801 cataaaataa taacattaaa aaagcaaata aattagcgag agaatttttt gacaaaaaga
     1861 aagaagagtg atagagaaga agggaatgct tgaaaggatt ttgcatttat cccactttgt
     1921 acaagaaagc tgggtttact tgctggaagt gtacttggtg acggccttgg ttccctcaga
     1981 cacggcgtgc ttggcaagtt ctcctgggag aatcaaacgg acagcggttt gaatttcgcg
     2041 ggatgagatc gttgagcgtt tgttgtaatg agcaagacgg gaagcttccg aagcgatgcg
     2101 ttcgaataca tcgttgacga aggagttcat gatagacatg gccttggagg agactccggt
     2161 gtctgggtga acttgcttga gaacacggta gatgtagacg gagtacgatt ccttgcgggc
     2221 atgacgtctc ttctttccgt cctttggctt ggcaacgacg gtcttggcgg ccttcttggc
     2281 tcccttggca gatggctttg gtggactagt tctagagcgg ccgccaccgg tggagctcca
     2341 ccgatccccc gggcatttgt atagttcatc catgccatgt gtaatcccag cagctgttac
     2401 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg
     2461 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
     2521 ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa ttttgaagtt
     2581 ctgaaaattt aaatcagatt agttagttag taaaatcatg tttaaactta caactttgat
     2641 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
     2701 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
     2761 aacaagggta tcaccttcaa acttgacttc agcacctgaa aatttaaata tgtatggtta
     2821 gttagtaccg aactgtttaa acttacgtgt cttgtagttc ccgtcatctt tgaaaaatat
     2881 agttctttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat gccgtttcat
     2941 atgatctggg tatctcgaga agcattgaac accataacag aaagtagtga caagtgttgg
     3001 ctgaaaattt aaataatcag ggttagttag tatatatatg tttaaactta cccatggaac
     3061 aggtagtttt ccagtagtgc aaataaattt aagggtaagt tttccgtatg ttgcatcacc
     3121 ttcaccctct ccactgacag aaaatttgtg cccattaaca tcaccatcta attcaacaag
     3181 aattgggaca actccagtga aaagttcttc tcctttactc atggagcctg cttttttgta
     3241 caaacttgtc atttctgtgt ctgacgatcg agggttaaaa tgaaaagtgg tggagagaag
     3301 tcaaccgctc ggtgggatgc gcgagcctgg agataacttt gattttcggc gagcccttct
     3361 ttttccctgg gtgggtggat tgggaggcgg ggatatccaa ttgtaattct aggtagctga
     3421 aaagtgtctg cacactgttc tctgaccatc gacgcaggaa aagtctcttt ctcaagtgca
     3481 aatagcaaaa atcttctttc ttccccccca tcttccgagc aaggcgcccc cacggatagt
     3541 tgctcgctgg cggtagggag cgaagagagc agagaacctc aacgggatag tggggatacg
     3601 agaaaagaaa cggcatttta ggttaactaa agatagtgaa gaagccggct caatgcagag
     3661 aggcagacat ccgacggcaa acaacgagag aattttatcc agaacaatga gtacagtgtg
     3721 tgcgcgcaaa gtgctcttcc tgccattttg ctactcttat ccacacaacc actttagcga
     3781 ttaccaacct atcttgagac ttatctttat tttctcttct tttgccgacg aaacgggaga
     3841 gaggggcaaa aaatgaagaa tcgggggcac tgattaacac acttactttt ttttaggctg
     3901 aagcacacat tgctgttcgg ggtgtaaagt tcagcagaat tcaaagcaaa agaccacccg
     3961 catcggtagt caatattgat ctggaagatt attaaaaatt gtcagagtga gccatatgca
     4021 tatgaattta attcatacat aacatttata agacaggaat aaataccaaa gtgaagttat
     4081 tatttggtcg attatcactt tagcatagtg aaagtatccc tcaatgtctc tacttgtaaa
     4141 ctcagttgat ttacaaacgt tgttttattg attataaaat tagttttatg gacatttaaa
     4201 gcaaaggata ctactcagat ataaaatgca acttttctat acaaagttga tagcttggcg
     4261 gatccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     4321 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     4381 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     4441 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     4501 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4561 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     4621 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     4681 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4741 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4801 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4861 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4921 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4981 gatccttgag agttttcgcc ccgaagaacg ttttccaatg
//
