LOCUS       sequence-421773-        1716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes S.cerevisiae SUC2 (Invertase) pre- signal peptide as a Type 
            3a' part to be used in the yeast secretion/display (YSD) extension 
            of the MoClo yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD004
FEATURES             Location/Qualifiers
     source          1..1716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      199..233
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(415..1003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(495..514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1185..1716,1..128)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1251..1270)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
       61 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
      121 gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga tagatccagt aatgacctca
      181 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtctg
      241 gtctcaagca gatattttgg ctgcaaaacc agccaaaagg aaaaggaaag cttgcaaaag
      301 catatgagac cgactacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      841 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgat
     1081 tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     1141 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     1201 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     1261 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     1321 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     1381 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     1441 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     1501 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     1561 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgttt
     1621 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1681 tcttcgcccc cgttttcacc atgggcaaat attata
//
