LOCUS       sequence-421774-        1935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a hybrid S. cerevisiae OST1 pre- MFalpha1 pro- signal 
            peptide (OST1MFapp) as a Type 3a' part to be used in the yeast 
            secretion/display (YSD) extension of the MoClo yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD023
FEATURES             Location/Qualifiers
     source          1..1935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(300..334)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(405..1064)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     979..998
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1246..1834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1735..1754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgaggcagg tttggttctc ttggattgtg ggattgttcc tatgtttttt
       61 caacgtgtct tctgctgctc cagttaacac caccaccgaa gacgaaaccg ctcaaatccc
      121 agctgaagct gttatcggat acttggactt ggaaggtgac ttcgacgttg ctgtcttgcc
      181 attctctaac tctaccaaca acggtttgct cttcattaac accaccatcg cttctatcgc
      241 tgctaaggaa gaaggtgttt ccttggacaa gagagaagct gatgcttgag accagaccaa
      301 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      361 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      421 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      481 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      541 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      601 ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta
      661 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      721 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
      781 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      841 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
      901 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      961 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     1021 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     1081 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1141 aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc
     1201 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1261 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1321 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1381 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1441 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1501 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1561 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1621 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1681 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1741 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1801 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1861 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1921 tgtggataac cgtag
//
