LOCUS       sequence-421775-        1989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the S. cerevisiae YAR066W Translational Fusion Partner 
            Sequence as a Type 3a' part to be used in the yeast 
            secretion/display (YSD) extension of the MoClo yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD041
FEATURES             Location/Qualifiers
     source          1..1989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     71..90
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      338..926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     827..846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1381..1415)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1486..1989,1..156))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
       61 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
      121 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
      181 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      241 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
      301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      961 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1021 accgtagtcg gtctcatatg ttcaatcgtt ttaacaaatt ccaagctgct gtcgctttgg
     1081 ccctactctc tcgcggcgct ctcggtgact cttacaccaa tagcacctcc tccgcagact
     1141 tgagttctat cacttcagtc tcgtcagcta gtgcaagtgc caccgcttcc gactcacttt
     1201 cttccagtga cggtaccgtt tatttgccat ccacaacaat tagcggtgat ctcacagtta
     1261 ctggtaaagt aattgcaacc gaggccgtgg aagtcgctgc cggtggtaag ttgactttac
     1321 ttgacggtga aaaatacgtc ttctcatctg atctagacaa acgtgcttga gaccagacca
     1381 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1441 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     1501 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1561 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1621 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1681 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
     1741 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1801 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     1861 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1921 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1981 aaacttgtg
//
