LOCUS       sequence-421778-        1728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes S. cerevisiae OST1 pre- signal peptide as a Type 3a' part to
            be used in the yeast secretion/display (YSD) extension of the MoClo 
            yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD007
FEATURES             Location/Qualifiers
     source          1..1728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      219..253
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(447..1035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(527..546)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1217..1728,1..148)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1283..1302)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
       61 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
      121 ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga
      181 tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc
      241 gggcgttttt tattggtctg gtctcaagca gaagacacgt tgaaaaaaca taggaacaat
      301 cccacaatcc aagagaacca aacctgcctc atcatatgag accgactacg gttatccaca
      361 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      421 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      481 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      541 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      781 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      841 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      901 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      961 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1021 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1081 gaaaactcac gttaagggat tttggtcatg attgatcggg cacgtaagag gttccaactt
     1141 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
     1201 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     1261 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     1321 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     1381 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat
     1441 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     1501 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     1561 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     1621 taaagggttt attgagaata tgtttttcgt ttcagccaat ccctgggtga gtttcaccag
     1681 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgtttt
//
