LOCUS       sequence-421783-        1734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes S.cerevisiae ECM14 pre- signal peptide as a Type 3a' part to
            be used in the yeast secretion/display (YSD) extension of the MoClo 
            yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD003
FEATURES             Location/Qualifiers
     source          1..1734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      210..798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     699..718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(998..1032)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1103..1734,1..28))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1677..1696
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
       61 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
      121 cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
      181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
      241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      841 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt
      901 cggtctcata tgatgttgca catgaattcg ttgtggggtt gttttctttt cgttttacta
      961 gccgtgacag gtgctgtgca tgcttgagac cagaccaata aaaaacgccc ggcggcaacc
     1021 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     1081 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     1141 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     1201 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     1261 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     1321 tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     1381 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     1441 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     1501 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     1561 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     1621 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     1681 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acgg
//
