LOCUS       sequence-421785-        2716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the HA-tagged S. cerevisiae Sed1p yeast surface display 
            anchor protein as a Type 4a part to be used in the yeast 
            secretion/display (YSD) extension of the MoClo yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD083
FEATURES             Location/Qualifiers
     source          1..2716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..34)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      679..713
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1700..1720)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(1724..1750)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      complement(1895..2483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1975..1994)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2665..2716,1..608)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
       61 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
      121 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga
      181 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
      241 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
      301 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
      361 ttattgagaa tatgtttttc gtttcagcca atccctgggt gagtttcacc agttttgatt
      421 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
      481 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
      541 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
      601 gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtctg
      721 gtctcagcca ctcgagttat aagaataaca tagcaacacc agccaaacct aaagcacctg
      781 gaacgacgac gttagcaccg ttactgttga tgacaacgga atgagaagaa gcagaggatg
      841 aaactgggac gactgtggat acggttagac ttgggttggc tgtggtttgt ttggtagtaa
      901 cacctgtttc tttggtggta gtgcccttag attcggtaac tgggacagaa gactcagggg
      961 cttcgctctt ttcaatagta catggacagt cagtgatagt caaagtggtt ggttcagtga
     1021 cggtgtaagt cttaccgttt gtggtgaaag tggttggttc tggacagtaa gtagtgtact
     1081 cagtgactac agtgtattcg gtggttgatg tggttgttgg cttttcaatg gtgcatggac
     1141 agtcagtgat agtcaatgtg gttggttcag tgacggtgta agtcttaccg tttgtggtga
     1201 aagtggttgg ttctggacag taagtagtat attcagtgac tacagtgtag tcagtggtgt
     1261 agtcagtaga tgggttgtaa ggaggagtgg tggtagtgtt gcttggtggc aaagatgtag
     1321 ttggtgggaa agctgaagtg gtaccgttgg ttggaagacc ggtggttgga gcttcagtag
     1381 tagtatcagt tggagcttca gtagaagtac cgttagttgg aagagcggtg gttggagctt
     1441 cagtagtagt atcagttgga gcttcagtag aggtaccgtt agttgggata gcagtggttg
     1501 gagcttcagt agaggtaccg ttagttggga tagcagtggt tggagcctct gttgaggttt
     1561 cagttggtgc agctgtgctg gtaccgttgt cggattctgg agcttcagaa gatgtgatag
     1621 ttactgagcc agaggaagtg gagatggaag aggaggaagt gacatcggtg gaagaagcag
     1681 atgtactgtt ggaaaattga gattgaaaat acaaattttc cttagcgtaa tctggaacat
     1741 cgtatgggta tgatccgcca ccaccagaac cccctccacc ggattgagac cgactacggt
     1801 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1861 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1921 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1981 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2041 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2101 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2161 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2221 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2281 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2341 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2401 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2461 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2521 agtggaacga aaactcacgt taagggattt tggtcatgat tgatcgggca cgtaagaggt
     2581 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     2641 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     2701 atatcccaat ggcatc
//
