LOCUS       sequence-421786-        1719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes S. cerevisiae MFalpha1 pre- signal peptide as a Type 3a' 
            part to be used in the yeast secretion/display (YSD) extension of 
            the MoClo yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD006
FEATURES             Location/Qualifiers
     source          1..1719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             79..738
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(145..164)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      809..843
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(1028..1616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1108..1127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
       61 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
      121 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
      181 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
      241 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt
      301 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
      361 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
      421 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
      481 cctaaagggt ttattgagaa tatgtttttc gtttcagcca atccctgggt gagtttcacc
      541 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
      601 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
      661 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
      721 tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga tagatccagt
      781 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
      841 tattggtctg gtctcaagca agtgcggagg atgctgcgaa taaaactgca gtaaaaattg
      901 aaggaaatct catcatatga gaccgactac ggttatccac agaatcaggg gataacgcag
      961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1021 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1441 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1621 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1681 ttttggtcat gattgatcgg gcacgtaaga ggttccaac
//
