LOCUS       sequence-421792-        1716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes S.cerevisiae AGA2 pre- signal peptide as a Type 3a' part to 
            be used in the yeast secretion/display (YSD) extension of the MoClo 
            yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD001
FEATURES             Location/Qualifiers
     source          1..1716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      153..741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     642..661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(923..957)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1028..1687)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1602..1621
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
       61 ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
      121 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      181 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      241 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      301 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      361 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      421 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      481 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      541 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      601 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      661 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      721 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      781 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      841 agtcggtctc atatgatgca gttacttcgc tgtttttcaa tattttctgt tattgcttca
      901 gttcttgctt gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
      961 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     1021 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1081 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1141 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1201 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa
     1261 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1321 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1381 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1441 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     1501 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1561 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1621 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1681 tctccatttt agcttcctta gctcctgaaa atctcg
//
