LOCUS       sequence-421793-        3844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the S. cerevisiae Aga1p yeast surface display anchor protein
            as a Type 3 part to be used in the yeast secretion/display (YSD) 
            extension of the MoClo yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD080
FEATURES             Location/Qualifiers
     source          1..3844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1065..1099)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1170..1829)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1744..1763
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2011..2599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2500..2519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaatatatca ccaaatcctc aatttctact actatcccat cattttccat gtctacatat
       61 ttcaccactg ttagtggagt cactacaatg tatacgacat ggtgtcctta tagctctgaa
      121 tctgagacta gcacattaac cagtatgcat gaaacggtta caacagacgc tacagtctgc
      181 actcacgagt cttgcatgcc ctcgcagaca acaagtttga ttacatcttc tataaaaatg
      241 tccactaaaa acgtcgcaac ttctgtaagc acctcaacgg ttgaatcctc atatgcatgc
      301 tccacatgtg ctgaaacgtc acactcgtat tcttccgtgc aaacagcttc atcaagttct
      361 gtaacacagc agaccacatc cacaaagagt tgggtaagtt caatgacaac ttcggatgaa
      421 gatttcaata agcacgctac cggtaagtat catgtaacat cttcaggtac ctcaaccatt
      481 tcgactagtg taagtgaagc cacgagtaca tcaagcattg actcagaatc tcaagaacaa
      541 tcatcacact tattatcgac atcggtcctt tcatcctcct ccttgtctgc tacattatcc
      601 tctgacagta ctattttgct attcagttct gtatcatcac taagtgtcga acagtcacca
      661 gttaccacac ttcaaatttc ttcaacatca gagattttac aacccacttc ttccacagct
      721 attgctacaa tatctgcctc tacatcatca ctttccgcaa catctatctc tacaccatct
      781 acctctgtgg aatcgactat tgaatcttca tcattgactc cgacggtatc ttctattttc
      841 ctctcatcat catctgctcc ctcttctcta caaacatctg ttaccactac agaagtttcc
      901 actacttcaa tctccataca ataccaaact tcatcaatgg taacaattag ccaatatatg
      961 ggcagtggat cgcaaacgcg tttgccatta ggaaagttgg tcttcgccat catggcagtt
     1021 gcttgcaatg taattttcag ttaataaatc ctgagaccag accaataaaa aacgcccggc
     1081 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     1141 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
     1201 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     1261 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     1321 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     1381 cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     1441 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     1501 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     1561 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     1621 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     1681 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     1741 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     1801 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     1861 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     1921 acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1981 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2041 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2101 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2161 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2221 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2281 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2341 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2401 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2461 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2521 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2581 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2641 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag
     2701 tcggtctcat atgacattat ctttcgctca ttttacctac ctgttcacaa tattgttggg
     2761 attaactaat attgccttgg catctgatcc agaaacgatt ctagtgacga taaccaagac
     2821 aaacgatgca aatggggttg ttacaactac agtttcaccc gcgctagtct ccacatccac
     2881 tatcgttcaa gctggcacta cgacattgta tacgacttgg tgtccattga cggtatccac
     2941 ttcatctgct gccgaaataa gtccttcaat atcgtacgct actaccctat ccagatttag
     3001 tactttgaca ttatctacag aagtctgctc ccatgaggca tgtccttcgt catcgacgtt
     3061 gccaaccacc accttatctg tgacttccaa gttcacttca tatatttgcc ctacttgtca
     3121 cacaaccgct atcagctcat tatccgaagt aggaactaca accgtggtat catccagcgc
     3181 cattgaacca tcaagtgcct ctataatctc acctgtcacc tctacacttt cgagtacaac
     3241 atcgtccaat ccaactacta cctccctaag ttcgacatct acatctccaa gctctacatc
     3301 tacatctcca agctctacat ctacctcatc aagttcgaca tctacctcat caagttcgac
     3361 atctacctca tcaagttcga catctacatc tccaagttcg acatccacat cttcaagttt
     3421 gacatccaca tcttcaagtt ctacatctac atcccaaagt tctacatcta cctcatcaag
     3481 ttcgacatct acatctccaa gctctacatc tacctcatca agttcaacat ctacatctcc
     3541 aagttctaaa tctacttctg caagctccac ttccacttct tcatattcaa catctacatc
     3601 cccaagtttg acttcttcat ctccaacttt ggcttccact tctccaagtt caacatctat
     3661 tagctctact tttactgatt caacttcatc ccttggctcc tctatagcat cttcatcaac
     3721 gtctgtgtca ttatacagcc catccacacc tgtttactcc gtcccttcga cttcgtcaaa
     3781 tgttgcaact ccttctatga cttcttcaac tgttgaaaca actgttagtt cacaaagttc
     3841 gtct
//
