LOCUS       sequence-421796-        2329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the HA-tagged S. cerevisiae Tip1p yeast surface display 
            anchor protein as a Type 4a part to be used in the yeast 
            secretion/display (YSD) extension of the MoClo yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD084
FEATURES             Location/Qualifiers
     source          1..2329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             369..1028
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(435..454)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1099..1133
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1733..1753)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(1757..1783)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      complement(join(1928..2329,1..187))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2008..2027)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
       61 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      121 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      181 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      241 acgttaaggg attttggtca tgattgatcg ggcacgtaag aggttccaac tttcaccata
      301 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
      361 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      421 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
      481 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
      541 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga
      601 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
      661 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
      721 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
      781 ttattgagaa tatgtttttc gtttcagcca atccctgggt gagtttcacc agttttgatt
      841 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
      901 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
      961 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     1021 gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga tagatccagt aatgacctca
     1081 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtctg
     1141 gtctcagcca ctcgagttat aacaataaag cagctgcacc tgcaacaaca gcaccgaaag
     1201 aggcagcgcc tgcattgact ctttgaccgg cattggaagc agtttcgact gtagaagtgc
     1261 tgacagaaga ggtagttgga gcgacagaag aagaaacggc cttagaagat gattcggcag
     1321 atgatgatgg ggcagcagaa gaagatgggg cagcagaaga ggatggagcg gcagaggatg
     1381 tagcttcgga agaagatgca gccttggaag aagatgcagc ctcggaagaa gatgcagcct
     1441 cggaagaagc tggggaaacg gaggcaagag cagcagcgat ttcagaactc aatcttgtgg
     1501 tgtaccatgg caatttaaca attgtcttag tgatagcatc aaagtccaat tcactaaaca
     1561 aggtagtgta agcgtcatcg gtgtaagtca tgacttgttg atacacactc aagacatcag
     1621 atggaatttg gaatccactg ttgccagttt ctagacccaa gtagtcagaa agatgagagt
     1681 tgatgtcacc gataatagct tgcaattcag cagtttcggc ggcgctggtg tcagattgaa
     1741 aatacaaatt ttccttagcg taatctggaa catcgtatgg gtatgatccg ccaccaccag
     1801 aaccccctcc accggattga gaccgactac ggttatccac agaatcaggg gataacgcag
     1861 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1921 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1981 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2041 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2101 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2161 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2221 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2281 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacaga
//
