LOCUS       sequence-421797-        2007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the S. cerevisiae 111 amino acid SCW4 Translational Fusion 
            Partner Sequence as a Type 3a' part to be used in the yeast 
            secretion/display (YSD) extension of the MoClo yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD043
FEATURES             Location/Qualifiers
     source          1..2007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     80..99
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      347..935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     836..855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1408..1442)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1513..2007,1..165))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
       61 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
      121 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
      181 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
      241 aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt
      301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
      361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
      421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
      481 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      961 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     1021 ctgtggataa ccgtagtcgg tctcatatgc gtctatctaa cctaattgct tctgcctctc
     1081 ttttatctgc tgctactctt gctgctcccg ctaaccacga acacaaggac aagcgtgctg
     1141 tggtcactac cactgttcaa aaacaaacca ctatcattgt taatggtgcc gcttcaactc
     1201 cagttgctgc tttggaagaa aatgctgttg tcaactccgc tccagctgcc gctaccagta
     1261 caacatcgtc tgctgcttct gtagctaccg ctgctgcttc ctcttctgag aacaactcac
     1321 aagtttctgc tgccgcatct ccagcctcca gctctgctgc tacatctact caatcttccc
     1381 tagacaaacg tgcttgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg
     1441 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     1501 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     1561 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1621 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1681 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac
     1741 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1801 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1861 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1921 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     1981 ggcaagaatg tgaataaagg ccggata
//
