LOCUS       sequence-42181-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Gunewardene MS, Subach FV, Gould TJ, Penoncello GP, Gudheti MV, 
            Verkhusha VV, Hess ST
  TITLE     Superresolution imaging of multiple fluorescent proteins with highly
            overlapping emission spectra in living cells.
  JOURNAL   Biophys J. 2011 Sep 21;101(6):1522-8. Epub 2011 Sep 20.
  PUBMED    21943434
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys 
            J."; date: "2011-09-21"; volume: "101(6)"; pages: "1522-8. Epub 2011
            Sep 20"
COMMENT     Sequence Label: pPAmKate-N1
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    31..111
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             119..832
                     /codon_start=1
                     /product="monomeric photoactivatable red fluorescent 
                     protein (Gunewardene et al., 2011)"
                     /label=PAmKate
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRGD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgagct
       61 caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat
      121 ggtgtctaag ggcgaagagc tgattaagga gaacatgcac atgaagctgt acatggaggg
      181 caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
      241 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
      301 cctggctacc agcttcatgt acggcagcaa gaccttcatc aaccacaccc agggcatccc
      361 cgacttctgg aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
      421 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
      481 caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac
      541 actcggctgg gaggccaaca ccgagatgct gtaccccgct gacggcggcc tggaaggcag
      601 aggggacatg gccctgaagc tcgtgggcgg gggccacctg atctgcaact tgaagaccac
      661 atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggaccg
      721 cagactggaa agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc
      781 tgtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatt aaagcggccg
      841 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      901 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      961 tttattgcag cttataatgg ttacaa
//
