LOCUS       sequence-421832-        1521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 
            (codon-optimized for insect cell expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Mahoney JP, Bruguera ES, Vasishtha M, Killingsworth LB, Kyaw S, Weis
            WI
  TITLE     PI(4,5)P(2)-stimulated positive feedback drives the recruitment of 
            Dishevelled to Frizzled in Wnt-beta-catenin signaling.
  JOURNAL   Sci Signal. 2022 Aug 23;15(748):eabo2820. doi: 
            10.1126/scisignal.abo2820. Epub 2022 Aug 23.
  PUBMED    35998232
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2022-08-23"; volume: "15(748):eabo2820. doi"; 
            pages: " 10.1126/scisignal.abo2820. Epub 2022 Aug 23"
COMMENT     Sequence Label: FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
FEATURES             Location/Qualifiers
     source          1..1521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(174..185)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gactacaagg acgatgatga cgccatggag gagcgccgct gcgacccaat ccgcattgcc
       61 atgtgccaga acctgggata caatgtgacc aagatgccaa acctggtggg ccacgagctg
      121 cagacagatg ccgagctgca gctgacaacc ttcacccccc tgatccagta cggctgcagc
      181 tcccagctgc agttctttct gtgcagcgtg tatgtgccga tgtgcaccga gaagatcaac
      241 attccaatcg gaccatgcgg aggaatgtgc ctgtccgtga agcgccgctg cgagccagtg
      301 ctgcgcgagt tcggctttgc ctggcccgat accctgaatt gctcgaagtt ccccccgcag
      361 aacgatcaca atcatatgtg catggaggga ccaggagacg aggaggtgcc gctgccacac
      421 aagacgccaa tccagcccgg agaggagtgc cattcggtgg gctccaactc ggaccagtac
      481 atttgggtga agcgcagcct gaattgcgtg ctgaagtgcg gatacgatgc cggcctgtat
      541 agtcgcagcg ccaaggagtt caccgacatt tggatggccg tgtgggccag tctgtgcttc
      601 atcagcacga catttacagt gctgaccttc ctgattgatt cgagtcgctt tagctacccg
      661 gagcgcccaa tcattttcct gtccatgtgc tacaacatct attcgattgc ctatatcgtg
      721 cgcctgacag tgggacgcga gcgcatctcc tgcgactttg aggaggccgc cgagccagtg
      781 ctgattcagg agggactgaa gaatacgggc tgcgccatca ttttcctgct gatgtacttc
      841 tttggcatgg ccagctccat ttggtgggtg atcctgaccc tgacgtggtt tctggccgcc
      901 ggactgaagt ggggacacga ggccatcgag atgcactcga gttacttcca tattgccgcc
      961 tgggccatcc cagccgtgaa gaccattgtg atcctgatta tgcgcctggt ggatgccgac
     1021 gagctgacgg gactgtgcta cgtgggcaac cagaatctgg acgccctgac aggctttgtg
     1081 gtggcccccc tgttcaccta tctggtgatt ggaacgctgt ttatcgccgc cggactggtg
     1141 gccctgttca agatccgcag caacctgcag aaggatggaa caaagaccga caagctggag
     1201 cgcctgatgg tgaagattgg cgtgttctcc gtgctgtaca cagtgcccgc cacctgcgtg
     1261 attgcctgct acttttatga gatctcgaac tgggccctgt tccgctatag tgccgatgac
     1321 agcaatatgg ccgtggagat gctgaagatc ttcatgtccc tgctggtggg aattacctcc
     1381 ggcatgtgga tctggtcggc caagacgctg cacacatggc agaagtgcag taaccgcctg
     1441 gtgaatagcg gaaaggtgaa gcgcgagaag cgcggcaatg gctgggtgaa gcccggaaag
     1501 ggaaacgaga ccgtggtgta a
//
