LOCUS       sequence-421842-       15388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transfer plasmid for lentiviral vector production expressing Hs 
            ATP13A2 WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15388)
  AUTHORS   Colijn MA, Vrijsen S, Au PYB, Abou El Asrar R, Houdou M, Van den 
            Haute C, Sarna J, Montgomery G, Vangheluwe P
  TITLE     Kufor-Rakeb syndrome-associated psychosis: a novel loss-of-function 
            ATP13A2 variant and response to antipsychotic therapy.
  JOURNAL   Neurogenetics. 2024 Jul 18. doi: 10.1007/s10048-024-00767-7.
  PUBMED    39023817
REFERENCE   2  (bases 1 to 15388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neurogenetics. 2024 Jul 18. doi: 10.1007/s10048-024-00767-7."
COMMENT     Sequence Label: pLenti HsATP13A2 WT (PAM) _ Hygro
FEATURES             Location/Qualifiers
     source          1..15388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1115..1134)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1348..1367)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1467..1489
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1527..1545)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1613..1717
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1718..1786,1787..2578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1936..1955)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2749..3337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3238..3257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3491..3508
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3578..3598)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3583..3912
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3763..3898
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5046..5111
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5241..5261
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5686..5820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5723..5742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5777..5796
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5988..6621
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6668..6793
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7286..7519
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7704..7748
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8041..8158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8217..8520
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8521..8724
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8675..8695
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8721..8745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(12551..12568)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12778..12797
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             12965..13990
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             12965..13990
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    14037..14625
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(14090..14110)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14508..14519)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             14795..14975
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatcttctg acacaaattc catttactcc tcaccctatg actcagtcga atatatcaaa
       61 gttatggaca ttatgctaag taacaaatta cccttttata tagtaaatac tgagtagatt
      121 gagagaagaa attgtttgca aacctgaata gcttcaagaa gaagagaagt gaggataaga
      181 ataacagttg tcatttaaca agttttaaca agtaacttgg ttagaaaggg attcaaatgc
      241 ataaagcaag ggataaattt ttctggcaac aagactatac aatataacct taaatatgac
      301 ttcaaataat tgttggaact tgataaaact aattaaatat tattgaagat tatcaatatt
      361 ataaatgtaa tttactttta aaaagggaac atagaaatgt gtatcattag agtagaaaac
      421 aatccttatt atcacaattt gtcaaaacaa gtttgttatt aacacaagta gaatactgca
      481 ttcaattaag ttgactgcag attttgtgtt ttgttaaaat tagaaagaga taacaacaat
      541 ttgaattatt gaaagtaaca tgtaaatagt tctacatacg ttcttttgac atcttgttca
      601 atcattgatc gaagttcttt atcttggaag aatttgttcc aaagactctg aaataaggaa
      661 aacaatctat tatatagtct cacacctttg ttttactttt agtgatttca atttaataat
      721 gtaaatggtt aaaatttatt cttctctgag atcatttcac attgcagata gaaaacctga
      781 gactggggta atttttatta aaatctaatt taatctcaga aacacatctt tattctaaca
      841 tcaatttttc cagtttgata ttatcatata aagtcagcct tcctcatctg caggttccac
      901 aacaaaaatc caaccaactg tggatcaaaa atattgggaa aaaattaaaa atagcaatac
      961 aacaataaaa aaatacaaat cagaaaaaca gcacagtata acaactttat ttagcattta
     1021 caatctatta ggtattataa gtaatctagc cagatcctct acgccggacg catcgtggcc
     1081 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     1141 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     1201 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     1261 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     1321 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     1381 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     1441 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     1501 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     1561 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     1621 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1681 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1741 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1801 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1861 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1921 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     1981 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     2041 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     2101 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     2161 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2221 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2281 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2341 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2401 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2461 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2521 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2581 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2641 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2701 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2761 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2821 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2881 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2941 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3001 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3061 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3121 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3181 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3241 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3301 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3361 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3421 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3481 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     3541 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
     3601 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3661 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3721 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3781 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3841 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3901 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     3961 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     4021 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     4081 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     4141 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     4201 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
     4261 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     4321 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     4381 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
     4441 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
     4501 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
     4561 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
     4621 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
     4681 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
     4741 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
     4801 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
     4861 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
     4921 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
     4981 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     5041 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     5101 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     5161 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     5221 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     5281 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     5341 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     5401 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
     5461 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
     5521 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
     5581 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     5641 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     5701 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5761 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     5821 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     5881 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
     5941 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
     6001 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     6061 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     6121 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     6181 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     6241 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     6301 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     6361 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     6421 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6481 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6541 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6601 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     6661 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     6721 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     6781 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     6841 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     6901 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     6961 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     7021 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     7081 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     7141 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     7201 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     7261 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     7321 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     7381 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     7441 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     7501 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     7561 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     7621 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     7681 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     7741 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     7801 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     7861 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     7921 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     7981 gattagtgaa cggatctcga cggtatcgcc gaattcacaa atggcagtat tcatccacaa
     8041 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     8101 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     8161 ggtttattac agggacagca gagatccact ttggggcgat aagcttggga gttccgcgtt
     8221 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     8281 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     8341 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     8401 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     8461 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     8521 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     8581 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     8641 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     8701 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     8761 ccacgctgtt ttgacctcca tagaagacac cgactctagc tagaggatct tctagaagat
     8821 ctgccaccat gagcgcagac agcagccctc tcgtgggcag cacgcccaca ggatacggaa
     8881 ctctgacgat agggacatca atagatcccc tcagctcctc agtttcatcc gtgaggctca
     8941 gcggctactg tggcagtcca tggagggtca tcggctatca cgtcgtggtc tggatgatgg
     9001 ctgggatccc tttgctgctc ttccgttgga agcccctgtg gggggtgcgg ctgcggctcc
     9061 ggccctgcaa cctggcccac gccgaaacac tcgttatcga aataagagac aaagaggata
     9121 gttcctggca gctcttcact gtccaggtgc agactgaggc catcggcgag ggcagcctgg
     9181 agccgtcccc acagtcccag gcagaggatg gccggagcca ggcggcagtt ggggcggtac
     9241 cagagggtgc ctggaaggat acggcccagc tccacaagag cgaggaggcg aagcgggtgc
     9301 tgcggtatta cctcttccag ggccagcgct atatctggat cgagacccag caagccttct
     9361 accaggtcag cctcctggac catggccgct cttgtgacga cgtccaccgc tcccgccatg
     9421 gcctcagcct ccaggaccaa atggtgagga aggccattta cggccccaac gtgatcagca
     9481 taccggtcaa gtcctacccc cagctgctgg tggacgaggc actgaacccc tactatgggt
     9541 tccaggcctt cagcatcgcg ctgtggctgg ctgaccacta ctactggtac gccctgtgca
     9601 tcttcctcat ttcctccatc tccatctgcc tgtcgctgta caagaccaga aagcaaagcc
     9661 agactctaag ggacatggtc aagttgtcca tgcgggtgtg cgtgtgccgg ccagggggag
     9721 aggaagagtg ggtggactcc agtgagctag tgcccggaga ctgcctggtg ctgccccagg
     9781 agggtgggct gatgccctgt gatgccgccc tggtggccgg cgagtgcatg gtgaatgaga
     9841 gctctctgac aggagagagc attccagtgc tgaagacggc actgccggag gggctggggc
     9901 cctactgtgc agagacacac cggcggcaca cactcttctg cgggaccctc atcttgcagg
     9961 cccgggccta tgtgggaccg cacgtcctgg cagtggtgac ccgcacaggg ttctgcacgg
    10021 caaaaggggg cctggtgagc tccatcttgc acccccggcc catcaacttc aagttctata
    10081 aacacagcat gaagtttgtg gctgccctct ctgtcctggc tctcctcggc accatctaca
    10141 gcatcttcat cctctaccga aaccgggtgc ctctgaatga gattgtaatc cgggctctcg
    10201 acctggtgac cgtggtggtg ccacctgccc tgcctgctgc catgactgtg tgcacgctct
    10261 acgcccagag ccgactgcgg agacagggca ttttctgcat ccacccactg cgcatcaacc
    10321 tggggggcaa gctgcagctg gtgtgtttcg acaagacggg caccctcact gaggacggct
    10381 tagacgtgat gggggtggtg cccctgaagg ggcaggcatt cctgcccctg gtcccagagc
    10441 ctcgccgcct gcctgtgggg cccctgctcc gagcactggc cacctgccat gccctcagcc
    10501 ggctccagga cacccccgtg ggcgacccca tggacttgaa gatggtggag tctactggct
    10561 gggtcctgga ggaagagccg gctgcagact cagcatttgg gacccaggtc ttggcagtga
    10621 tgagaccccc actttgggag ccccagctgc aggcaatgga ggagcccccg gtgccagtca
    10681 gcgtcctcca ccgcttcccc ttctcttcgg ctctgcagcg catgagtgtg gtggtggcgt
    10741 ggccaggggc cactcagccc gaggcctacg tcaaaggctc cccggagctg gtggcagggc
    10801 tctgcaaccc cgagacagtg cccaccgact tcgcccagat gctgcagagc tatacagctg
    10861 ctggctaccg tgtcgtggcc ctggccagca agccactgcc cactgtgccc agcctggagg
    10921 cagcccagca actgacgagg gacactgtgg aaggagacct gagcctcctg gggctgctgg
    10981 tcatgaggaa cctactgaag ccgcagacaa cgccagttat ccaggctctg cgaaggaccc
    11041 gcatccgcgc cgtcatggtg acaggggaca acctgcagac agcggtgact gtggcccggg
    11101 gctgtggcat ggtggccccc caggagcatc tgatcatcgt ccacgccacc caccctgagc
    11161 ggggtcagcc tgcctctctc gagttcctgc cgatggagtc ccccacagcc gtgaatggcg
    11221 ttaaggatcc tgaccaggct gcaagctaca ccgtggagcc agacccccga tccaggcacc
    11281 tggccctcag cgggcccacc tttggtatca ttgtgaagca cttccccaag ctgctgccca
    11341 aggtcctggt ccagggcact gtctttgccc gcatggcccc tgagcagaag acagagctgg
    11401 tgtgcgagct acagaagctt cagtactgcg tgggcatgtg cggagacggc gccaatgact
    11461 gtggggccct gaaggcggct gatgtcggca tctcgctgtc ccaggcagaa gcctcagtgg
    11521 tctcaccctt cacctcgagc atggccagta ttgagtgcgt gcccatggtc atcagggagg
    11581 ggcgctgttc ccttgacact tcattcagcg tcttcaagta catggctctg tacagcctga
    11641 cccagttcat ctccgtcctg atcctctaca cgatcaacac caacctgggt gacctgcagt
    11701 tcctggccat cgacctggtc atcaccacca cagtggcagt gctcatgagc cgcacggggc
    11761 cagcgctggt cctgggacgg gtgcggccac cgggggcgct gctcagcgtg cccgtgctca
    11821 gcagcctgct gctgcagatg gtcctggtga ccggcgtgca gctagggggc tacttcctga
    11881 ccctggccca gccatggttc gtgcctctga acaggacagt ggccgcacca gacaacctgc
    11941 ccaactacga gaacaccgtg gtcttctctc tgtccagctt ccagtacctc atcctggctg
    12001 cagccgtgtc caagggggcg cccttccgcc ggccgctcta caccaatgtg cccttcctgg
    12061 tggccctggc gctcctgagc tccgtcctgg tgggtcttgt cctggtcccc ggcctcctgc
    12121 aggggccgct ggcgctgagg aacatcactg acaccggctt caagctgctg ctgctgggtc
    12181 tggtcaccct caacttcgtg ggggccttca tgctggagag cgtgctagac cagtgcctcc
    12241 ccgcctgcct gcgccgcctc cggcccaagc gggcctccaa gaagcgcttc aagcagctgg
    12301 aacgagagct ggccgagcag ccctggccgc cgctgcccgc cggccccctg aggtagacta
    12361 gcggcgcgcc gtcgagaagc tttttgaatt ccgccccccc cccctaacgt tactggccga
    12421 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
    12481 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
    12541 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
    12601 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
    12661 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
    12721 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
    12781 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
    12841 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
    12901 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgatcagca
    12961 cgtgatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
    13021 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
    13081 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
    13141 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
    13201 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
    13261 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
    13321 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
    13381 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
    13441 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
    13501 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
    13561 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
    13621 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
    13681 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
    13741 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
    13801 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
    13861 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
    13921 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
    13981 aaaggaatag gtcgagcact agtccccggg accgcggcac gcgttgaatt cccgataatc
    14041 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
    14101 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
    14161 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
    14221 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
    14281 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
    14341 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
    14401 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
    14461 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
    14521 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
    14581 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg gaattctgtc
    14641 gactggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
    14701 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaaaat cgtcgagaga
    14761 tgctgcatat aagcagctgc tttttgcttg tactgggtct ctctggttag accagatctg
    14821 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    14881 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    14941 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
    15001 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattataa
    15061 tagatttagc aggaattgaa ctaggagtgg agcacacagg caaagctgca gaagtacttg
    15121 gaagaagcca ccagagatac tcacgattct gcacatacct ggctaatccc agatcctaag
    15181 gattacatta agtttactaa catttatata atgatttata gtttaaagta taaacttatc
    15241 taatttacta ttctgacaga tattaattaa tcctcaaata tcataagaga tgattactat
    15301 tatccccatt taacacaaga ggaaactgag agggaaagat gttgaagtaa ttttcccaca
    15361 attacagcat ccgttagtta cgactcta
//
