LOCUS       sequence-421844-        7039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV construct expressing HA-hM3Dq-dTomato driven by VIP 
            interneuron-targeting enhancer. Alias: pAAV_YWE4.2_Gq_dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Furlanis E, Dai M, Leyva Garcia B, Tran T, Vergara J, Pereira A, 
            Gorissen BL, Wills S, Vlachos A, Hairston A, Dwivedi D, Du S, 
            McMahon J, Huang S, Morabito A, Vazquez A, Kim S, Lee AT, Chang EF, 
            Razzaq T, Qazi A, Vargish G, Yuan X, Caccavano A, Hunt S, 
            Chittajallu R, McLean N, Hewitt L, Paranzino E, Rice H, Cummins AC, 
            Plotnikova A, Mohanty A, Tangen AC, Shin JH, Azadi R, Eldridge MAG, 
            Alvarez VA, Averbeck BB, Alyahyay M, Vallejo TR, Soheib M, Vattino 
            LG, MacGregor CP, Chatain CP, Banks E, Olah VJ, Naskar S, Hill S, 
            Liebergall S, Badiani R, Hyde L, Hanley E, Xu Q, Allaway KC, 
            Goldberg EM, Rowan MJM, Nowakowski TJ, Lee S, Favuzzi E, Kaeser PS, 
            Sjulson L, Batista-Brito R, Takesian AE, Ibrahim LA, Iqbal A, Pelkey
            KA, McBain CJ, Dimidschstein J, Fishell G, Wang Y
  TITLE     An enhancer-AAV toolbox to target and manipulate distinct 
            interneuron subtypes.
  JOURNAL   Neuron. 2025 May 21;113(10):1525-1547.e15. doi: 
            10.1016/j.neuron.2025.05.002.
  PUBMED    40403705
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.05"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1525-1547.e15"
COMMENT     Sequence Label: pAAV_BiVIPe4_Gq_dTomato
FEATURES             Location/Qualifiers
     source          1..7039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     intron          712..844
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             867..893
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2682..2738
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2751..2771
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2772..3470
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(2902..2920)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3170..3189
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3364..3387
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3525..4113
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3578..3598)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3996..4007)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4142..4223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4179..4198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4233..4252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4302..4442
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4302..4431
                     /label=AAV2 ITR
     rep_origin      4517..4972
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4604..4623)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4814..4835
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4989..5008)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5108..5130
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5254..5358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5359..5427,5428..6219)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5577..5596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6390..6978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6879..6898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcgat tattttaaat cctcattggt caggaatgtt
      181 tacattcata gtgtgaagca gagcctgaaa tgccaactta gtacttcagt gtgctgctaa
      241 attgactaag cgtgctgaac agtaatagct tggtactaga agcccaatgg cagggctcag
      301 tggccaaatg tttttattat tacgaagtaa taaggctaat aagcagtgtt gtgtgatgga
      361 aggcaggctt acttctattt aaagatggaa tgaacagctt tgatggggac aagcaaagaa
      421 cacaagaaca ttagctgtgt tccgatattc agtgcacata ggccagagct tggcaaacat
      481 ttctgtaatg gccagatgat aaacatttga ggattggaag actatagggt ctccaatgca
      541 accactaaaa tcgatgttta aacttcctgc agcccgggct gggcataaaa gtcagggcag
      601 agccatctat tgcttacatt tgcttctagc ctgcaagtcg aggagcgcag ccttccagaa
      661 gcagagcgcg gcgccttaag ctgcagaagt tggtcgtgag gcactgggca cgtaagtatc
      721 aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga
      781 ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc
      841 acagcgagct cgctagcgcc accatgtacc catacgatgt tccagattac gctaccttgc
      901 acaataacag tacaacctcg cctttgtttc caaacatcag ctcctcctgg atacacagcc
      961 cctccgatgc agggctgccc ccgggaaccg tcactcattt cggcagctac aatgtttctc
     1021 gagcagctgg caatttctcc tctccagacg gtaccaccga tgaccctctg ggaggtcata
     1081 ccgtctggca agtggtcttc atcgctttct taacgggcat cctggccttg gtgaccatca
     1141 tcggcaacat cctggtaatt gtgtcattta aggtcaacaa gcagctgaag acggtcaaca
     1201 actacttcct cttaagcctg gcctgtgccg atctgattat cggggtcatt tcaatgaatc
     1261 tgtttacgac ctacatcatc atgaatcgat gggccttagg gaacttggcc tgtgacctct
     1321 ggcttgccat tgactgcgta gccagcaatg cctctgttat gaatcttctg gtcatcagct
     1381 ttgacagata cttttccatc acgaggccgc tcacgtaccg agccaaacga acaacaaaga
     1441 gagccggtgt gatgatcggt ctggcttggg tcatctcctt tgtcctttgg gctcctgcca
     1501 tcttgttctg gcaatacttt gttggaaaga gaactgtgcc tccgggagag tgcttcattc
     1561 agttcctcag tgagcccacc attacttttg gcacagccat cgctggtttt tatatgcctg
     1621 tcaccattat gactatttta tactggagga tctataagga aactgaaaag cgtaccaaag
     1681 agcttgctgg cctgcaagcc tctgggacag aggcagagac agaaaacttt gtccacccca
     1741 cgggcagttc tcgaagctgc agcagttacg aacttcaaca gcaaagcatg aaacgctcca
     1801 acaggaggaa gtatggccgc tgccacttct ggttcacaac caagagctgg aaacccagct
     1861 ccgagcagat ggaccaagac cacagcagca gtgacagttg gaacaacaat gatgctgctg
     1921 cctccctgga gaactccgcc tcctccgacg aggaggacat tggctccgag acgagagcca
     1981 tctactccat cgtgctcaag cttccgggtc acagcaccat cctcaactcc accaagttac
     2041 cctcatcgga caacctgcag gtgcctgagg aggagctggg gatggtggac ttggagagga
     2101 aagccgacaa gctgcaggcc cagaagagcg tggacgatgg aggcagtttt ccaaaaagct
     2161 tctccaagct tcccatccag ctagagtcag ccgtggacac agctaagact tctgacgtca
     2221 actcctcagt gggtaagagc acggccactc tacctctgtc cttcaaggaa gccactctgg
     2281 ccaagaggtt tgctctgaag accagaagtc agatcactaa gcggaaaagg atgtccctgg
     2341 tcaaggagaa gaaagcggcc cagaccctca gtgcgatctt gcttgccttc atcatcactt
     2401 ggaccccata caacatcatg gttctggtga acaccttttg tgacagctgc atacccaaaa
     2461 ccttttggaa tctgggctac tggctgtgct acatcaacag caccgtgaac cccgtgtgct
     2521 atgctctgtg caacaaaaca ttcagaacca ctttcaagat gctgctgctg tgccagtgtg
     2581 acaaaaaaaa gaggcgcaag cagcagtacc agcagagaca gtcggtcatt tttcacaagc
     2641 gcgcacccga gcaggccttg tccggagata acggaagcgg agccactaac ttctccctgt
     2701 tgaaacaagc aggggatgtc gaagagaatc ccgggccatc tagaatggcc cccaagaaga
     2761 agaggaaggt ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     2821 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     2881 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     2941 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     3001 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     3061 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     3121 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     3181 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     3241 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     3301 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     3361 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     3421 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag taagtcgacg
     3481 gcgcgcccca cccctgcagg gaattcgata tcaagcttat cgataatcaa cctctggatt
     3541 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3601 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3661 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3721 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3781 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3841 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3901 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct
     3961 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4021 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4081 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat
     4141 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4201 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4261 agcttatcga taccgcatgc acgtgcggac cgagcggccg caggaacccc tagtgatgga
     4321 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     4381 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     4441 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     4501 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4561 acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc
     4621 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4681 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     4741 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4801 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
     4861 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
     4921 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     4981 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     5041 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     5101 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     5161 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5221 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5281 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5341 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5401 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5461 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5521 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5581 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5641 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5701 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5761 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5821 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5881 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5941 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6001 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6061 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6121 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6181 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7021 tggccttttg ctcacatgt
//
