LOCUS       sequence-421850-        7165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV construct expressing HA-hM3Dq-dTomato driven by chandelier 
            cell-targeting enhancer. Alias: pAAV_WDC0004_Gq_dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Furlanis E, Dai M, Leyva Garcia B, Tran T, Vergara J, Pereira A, 
            Gorissen BL, Wills S, Vlachos A, Hairston A, Dwivedi D, Du S, 
            McMahon J, Huang S, Morabito A, Vazquez A, Kim S, Lee AT, Chang EF, 
            Razzaq T, Qazi A, Vargish G, Yuan X, Caccavano A, Hunt S, 
            Chittajallu R, McLean N, Hewitt L, Paranzino E, Rice H, Cummins AC, 
            Plotnikova A, Mohanty A, Tangen AC, Shin JH, Azadi R, Eldridge MAG, 
            Alvarez VA, Averbeck BB, Alyahyay M, Vallejo TR, Soheib M, Vattino 
            LG, MacGregor CP, Chatain CP, Banks E, Olah VJ, Naskar S, Hill S, 
            Liebergall S, Badiani R, Hyde L, Hanley E, Xu Q, Allaway KC, 
            Goldberg EM, Rowan MJM, Nowakowski TJ, Lee S, Favuzzi E, Kaeser PS, 
            Sjulson L, Batista-Brito R, Takesian AE, Ibrahim LA, Iqbal A, Pelkey
            KA, McBain CJ, Dimidschstein J, Fishell G, Wang Y
  TITLE     An enhancer-AAV toolbox to target and manipulate distinct 
            interneuron subtypes.
  JOURNAL   Neuron. 2025 May 21;113(10):1525-1547.e15. doi: 
            10.1016/j.neuron.2025.05.002.
  PUBMED    40403705
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.05"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1525-1547.e15"
COMMENT     Sequence Label: pAAV_BiPVe4_Gq_dTomato
FEATURES             Location/Qualifiers
     source          1..7165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     intron          838..970
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             993..1019
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2808..2864
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2877..2897
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2898..3596
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(3028..3046)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3296..3315
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3490..3513
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3651..4239
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3704..3724)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4122..4133)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4268..4349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4305..4324)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4359..4378
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4428..4568
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4428..4557
                     /label=AAV2 ITR
     rep_origin      4643..5098
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4730..4749)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4940..4961
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5115..5134)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5234..5256
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5380..5484
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5485..5553,5554..6345)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5703..5722)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6516..7104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7005..7024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcgat ggtgtatttc ctgtgtgggc taatgtagct
      181 aactttgttt ggactccagg tttctgaggc agtttcaaat tcttgattta tgggttcatt
      241 tttggatcat ggcagcctcc ctgctgaaac ccccatagtt tcccttcaat aaggaaggct
      301 ggatgagatc acagctgagg aatcctggtg gtgtgtactg tgttatttct ctcctagtgc
      361 tttgcctaca cccttccatc atgcttacat ttggacctga aaataattgg ttgaagctgc
      421 tcctcacttt tagcctttta tccgagtttg acagtacatc accccaaata acacggacta
      481 taaatggctt gaacctaatg agtgctcttt gtggacaagc cgtccattct ccttttcttg
      541 agagggaaat taccggctcc taagcttcta tttccagttc tatagaatgt gggcgctaat
      601 ttgaaagcag cctcataaaa tggaagcaaa caggctaatt agaaatattt atgggcctgt
      661 tagtgaatat atgccatcga tgtttaaact tcctgcagcc cgggctgggc ataaaagtca
      721 gggcagagcc atctattgct tacatttgct tctagcctgc aagtcgagga gcgcagcctt
      781 ccagaagcag agcgcggcgc cttaagctgc agaagttggt cgtgaggcac tgggcacgta
      841 agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag
      901 agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt
      961 ctctccacag cgagctcgct agcgccacca tgtacccata cgatgttcca gattacgcta
     1021 ccttgcacaa taacagtaca acctcgcctt tgtttccaaa catcagctcc tcctggatac
     1081 acagcccctc cgatgcaggg ctgcccccgg gaaccgtcac tcatttcggc agctacaatg
     1141 tttctcgagc agctggcaat ttctcctctc cagacggtac caccgatgac cctctgggag
     1201 gtcataccgt ctggcaagtg gtcttcatcg ctttcttaac gggcatcctg gccttggtga
     1261 ccatcatcgg caacatcctg gtaattgtgt catttaaggt caacaagcag ctgaagacgg
     1321 tcaacaacta cttcctctta agcctggcct gtgccgatct gattatcggg gtcatttcaa
     1381 tgaatctgtt tacgacctac atcatcatga atcgatgggc cttagggaac ttggcctgtg
     1441 acctctggct tgccattgac tgcgtagcca gcaatgcctc tgttatgaat cttctggtca
     1501 tcagctttga cagatacttt tccatcacga ggccgctcac gtaccgagcc aaacgaacaa
     1561 caaagagagc cggtgtgatg atcggtctgg cttgggtcat ctcctttgtc ctttgggctc
     1621 ctgccatctt gttctggcaa tactttgttg gaaagagaac tgtgcctccg ggagagtgct
     1681 tcattcagtt cctcagtgag cccaccatta cttttggcac agccatcgct ggtttttata
     1741 tgcctgtcac cattatgact attttatact ggaggatcta taaggaaact gaaaagcgta
     1801 ccaaagagct tgctggcctg caagcctctg ggacagaggc agagacagaa aactttgtcc
     1861 accccacggg cagttctcga agctgcagca gttacgaact tcaacagcaa agcatgaaac
     1921 gctccaacag gaggaagtat ggccgctgcc acttctggtt cacaaccaag agctggaaac
     1981 ccagctccga gcagatggac caagaccaca gcagcagtga cagttggaac aacaatgatg
     2041 ctgctgcctc cctggagaac tccgcctcct ccgacgagga ggacattggc tccgagacga
     2101 gagccatcta ctccatcgtg ctcaagcttc cgggtcacag caccatcctc aactccacca
     2161 agttaccctc atcggacaac ctgcaggtgc ctgaggagga gctggggatg gtggacttgg
     2221 agaggaaagc cgacaagctg caggcccaga agagcgtgga cgatggaggc agttttccaa
     2281 aaagcttctc caagcttccc atccagctag agtcagccgt ggacacagct aagacttctg
     2341 acgtcaactc ctcagtgggt aagagcacgg ccactctacc tctgtccttc aaggaagcca
     2401 ctctggccaa gaggtttgct ctgaagacca gaagtcagat cactaagcgg aaaaggatgt
     2461 ccctggtcaa ggagaagaaa gcggcccaga ccctcagtgc gatcttgctt gccttcatca
     2521 tcacttggac cccatacaac atcatggttc tggtgaacac cttttgtgac agctgcatac
     2581 ccaaaacctt ttggaatctg ggctactggc tgtgctacat caacagcacc gtgaaccccg
     2641 tgtgctatgc tctgtgcaac aaaacattca gaaccacttt caagatgctg ctgctgtgcc
     2701 agtgtgacaa aaaaaagagg cgcaagcagc agtaccagca gagacagtcg gtcatttttc
     2761 acaagcgcgc acccgagcag gccttgtccg gagataacgg aagcggagcc actaacttct
     2821 ccctgttgaa acaagcaggg gatgtcgaag agaatcccgg gccatctaga atggccccca
     2881 agaagaagag gaaggtggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca
     2941 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     3001 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     3061 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     3121 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     3181 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     3241 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     3301 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     3361 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     3421 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     3481 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     3541 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtaag
     3601 tcgacggcgc gccccacccc tgcagggaat tcgatatcaa gcttatcgat aatcaacctc
     3661 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3721 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3781 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3841 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3901 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     3961 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4021 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg
     4081 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4141 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4201 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg
     4261 agagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4321 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4381 tgtatcagct tatcgatacc gcatgcacgt gcggaccgag cggccgcagg aacccctagt
     4441 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     4501 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     4561 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     4621 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4681 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
     4741 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4801 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     4861 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4921 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
     4981 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
     5041 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     5101 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     5161 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5221 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5281 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5341 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5401 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5461 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5521 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5581 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5641 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5701 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5761 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5821 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5881 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5941 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6001 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6061 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6121 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6181 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6241 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6301 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6361 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6421 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6481 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6541 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6601 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6661 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6721 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6781 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6841 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6901 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6961 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7021 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7081 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7141 ttttgctggc cttttgctca catgt
//
