LOCUS       sequence-421856-        7398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV construct expressing HA-hM3Dq-dTomato driven by SST 
            interneuron-targeting enhancer. Alias: pAAV_S9E10_Gq_dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Furlanis E, Dai M, Leyva Garcia B, Tran T, Vergara J, Pereira A, 
            Gorissen BL, Wills S, Vlachos A, Hairston A, Dwivedi D, Du S, 
            McMahon J, Huang S, Morabito A, Vazquez A, Kim S, Lee AT, Chang EF, 
            Razzaq T, Qazi A, Vargish G, Yuan X, Caccavano A, Hunt S, 
            Chittajallu R, McLean N, Hewitt L, Paranzino E, Rice H, Cummins AC, 
            Plotnikova A, Mohanty A, Tangen AC, Shin JH, Azadi R, Eldridge MAG, 
            Alvarez VA, Averbeck BB, Alyahyay M, Vallejo TR, Soheib M, Vattino 
            LG, MacGregor CP, Chatain CP, Banks E, Olah VJ, Naskar S, Hill S, 
            Liebergall S, Badiani R, Hyde L, Hanley E, Xu Q, Allaway KC, 
            Goldberg EM, Rowan MJM, Nowakowski TJ, Lee S, Favuzzi E, Kaeser PS, 
            Sjulson L, Batista-Brito R, Takesian AE, Ibrahim LA, Iqbal A, Pelkey
            KA, McBain CJ, Dimidschstein J, Fishell G, Wang Y
  TITLE     An enhancer-AAV toolbox to target and manipulate distinct 
            interneuron subtypes.
  JOURNAL   Neuron. 2025 May 21;113(10):1525-1547.e15. doi: 
            10.1016/j.neuron.2025.05.002.
  PUBMED    40403705
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.05"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1525-1547.e15"
COMMENT     Sequence Label: pAAV_BiSSTe10_Gq_dTomato
FEATURES             Location/Qualifiers
     source          1..7398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     intron          1071..1203
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             1226..1252
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3041..3097
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3110..3130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3131..3829
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(3261..3279)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3529..3548
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3723..3746
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3884..4472
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3937..3957)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4355..4366)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4501..4582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4538..4557)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4592..4611
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4661..4801
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4661..4790
                     /label=AAV2 ITR
     rep_origin      4876..5331
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4963..4982)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5173..5194
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5348..5367)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5467..5489
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5613..5717
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5718..5786,5787..6578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5936..5955)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6749..7337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7238..7257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcgat ctaggttcct gtctgcaagc atagcagggt
      181 atcattaata gtgtcaggag ttggctctct cccataggat gggtctcaag ttggggcaat
      241 cattggttgg ctgatccctt ggtctctgtc ccatctttat tcctgcacat cttgtaggca
      301 ggacaaattt tggtttgttc caaagtgatt tttaaagcat taccattttg tgttttgttg
      361 gtttaaaaca ctttagctat tctggtgggt gcgtgatcct gcctcactac ggtctcagtc
      421 tccatctcga tggtgactaa ggatgcctcc tatgcactta tttaccatct gaatggttcc
      481 tttatagaat gcatgactat tcaatttttt tgctcattgg acagcatttt aatgtgtgaa
      541 aggattatac atccttcaag atatggaaga aaaagcacaa agccctaaat tctgtgaatt
      601 cagatgtgta aaaaaagaca aggaagtgca aactatatca gcttggtgtc tcattctggg
      661 tggctgtcag agccctgtga gctgacttaa gaattgggtc cctgcaatgg gtgctggcac
      721 ttgcaagggg atcatgagct caaggccata ctgagctgca taatgagacc ttgtctcagt
      781 aactagactg gggttggagg tcagtactaa agggctagcc tagcctaaat tcccgagttc
      841 cacccccagc actataaaag gaaaaggagg aagaggtgga gggaaataaa gaaaaggaaa
      901 gccagtgtat cgatgtttaa acttcctgca gcccgggctg ggcataaaag tcagggcaga
      961 gccatctatt gcttacattt gcttctagcc tgcaagtcga ggagcgcagc cttccagaag
     1021 cagagcgcgg cgccttaagc tgcagaagtt ggtcgtgagg cactgggcac gtaagtatca
     1081 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
     1141 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     1201 cagcgagctc gctagcgcca ccatgtaccc atacgatgtt ccagattacg ctaccttgca
     1261 caataacagt acaacctcgc ctttgtttcc aaacatcagc tcctcctgga tacacagccc
     1321 ctccgatgca gggctgcccc cgggaaccgt cactcatttc ggcagctaca atgtttctcg
     1381 agcagctggc aatttctcct ctccagacgg taccaccgat gaccctctgg gaggtcatac
     1441 cgtctggcaa gtggtcttca tcgctttctt aacgggcatc ctggccttgg tgaccatcat
     1501 cggcaacatc ctggtaattg tgtcatttaa ggtcaacaag cagctgaaga cggtcaacaa
     1561 ctacttcctc ttaagcctgg cctgtgccga tctgattatc ggggtcattt caatgaatct
     1621 gtttacgacc tacatcatca tgaatcgatg ggccttaggg aacttggcct gtgacctctg
     1681 gcttgccatt gactgcgtag ccagcaatgc ctctgttatg aatcttctgg tcatcagctt
     1741 tgacagatac ttttccatca cgaggccgct cacgtaccga gccaaacgaa caacaaagag
     1801 agccggtgtg atgatcggtc tggcttgggt catctccttt gtcctttggg ctcctgccat
     1861 cttgttctgg caatactttg ttggaaagag aactgtgcct ccgggagagt gcttcattca
     1921 gttcctcagt gagcccacca ttacttttgg cacagccatc gctggttttt atatgcctgt
     1981 caccattatg actattttat actggaggat ctataaggaa actgaaaagc gtaccaaaga
     2041 gcttgctggc ctgcaagcct ctgggacaga ggcagagaca gaaaactttg tccaccccac
     2101 gggcagttct cgaagctgca gcagttacga acttcaacag caaagcatga aacgctccaa
     2161 caggaggaag tatggccgct gccacttctg gttcacaacc aagagctgga aacccagctc
     2221 cgagcagatg gaccaagacc acagcagcag tgacagttgg aacaacaatg atgctgctgc
     2281 ctccctggag aactccgcct cctccgacga ggaggacatt ggctccgaga cgagagccat
     2341 ctactccatc gtgctcaagc ttccgggtca cagcaccatc ctcaactcca ccaagttacc
     2401 ctcatcggac aacctgcagg tgcctgagga ggagctgggg atggtggact tggagaggaa
     2461 agccgacaag ctgcaggccc agaagagcgt ggacgatgga ggcagttttc caaaaagctt
     2521 ctccaagctt cccatccagc tagagtcagc cgtggacaca gctaagactt ctgacgtcaa
     2581 ctcctcagtg ggtaagagca cggccactct acctctgtcc ttcaaggaag ccactctggc
     2641 caagaggttt gctctgaaga ccagaagtca gatcactaag cggaaaagga tgtccctggt
     2701 caaggagaag aaagcggccc agaccctcag tgcgatcttg cttgccttca tcatcacttg
     2761 gaccccatac aacatcatgg ttctggtgaa caccttttgt gacagctgca tacccaaaac
     2821 cttttggaat ctgggctact ggctgtgcta catcaacagc accgtgaacc ccgtgtgcta
     2881 tgctctgtgc aacaaaacat tcagaaccac tttcaagatg ctgctgctgt gccagtgtga
     2941 caaaaaaaag aggcgcaagc agcagtacca gcagagacag tcggtcattt ttcacaagcg
     3001 cgcacccgag caggccttgt ccggagataa cggaagcgga gccactaact tctccctgtt
     3061 gaaacaagca ggggatgtcg aagagaatcc cgggccatct agaatggccc ccaagaagaa
     3121 gaggaaggtg gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg
     3181 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3241 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     3301 ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc
     3361 cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3421 gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac
     3481 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
     3541 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     3601 gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     3661 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac
     3721 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     3781 cgagggccgc caccacctgt tcctgtacgg catggacgag ctgtacaagt aagtcgacgg
     3841 cgcgccccac ccctgcaggg aattcgatat caagcttatc gataatcaac ctctggatta
     3901 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3961 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4021 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4081 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4141 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4201 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4261 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg
     4321 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4381 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4441 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc
     4501 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4561 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatca
     4621 gcttatcgat accgcatgca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     4681 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     4741 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     4801 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     4861 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4921 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     4981 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5041 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     5101 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5161 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     5221 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     5281 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     5341 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5401 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5461 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5521 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5581 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5641 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5701 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5761 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5821 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5881 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5941 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6001 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6061 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6121 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6181 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6241 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6301 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6361 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6421 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6481 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6541 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6601 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6661 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6721 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6781 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6841 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6901 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6961 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7021 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7081 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7141 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7201 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7261 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7321 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7381 ggccttttgc tcacatgt
//
