LOCUS       sequence-421857-        7269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV construct expressing HA-hM3Dq-dTomato driven by Lamp5 
            interneuron-targeting enhancer. Alias: pAAV_WDL0003_Gq_dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Furlanis E, Dai M, Leyva Garcia B, Tran T, Vergara J, Pereira A, 
            Gorissen BL, Wills S, Vlachos A, Hairston A, Dwivedi D, Du S, 
            McMahon J, Huang S, Morabito A, Vazquez A, Kim S, Lee AT, Chang EF, 
            Razzaq T, Qazi A, Vargish G, Yuan X, Caccavano A, Hunt S, 
            Chittajallu R, McLean N, Hewitt L, Paranzino E, Rice H, Cummins AC, 
            Plotnikova A, Mohanty A, Tangen AC, Shin JH, Azadi R, Eldridge MAG, 
            Alvarez VA, Averbeck BB, Alyahyay M, Vallejo TR, Soheib M, Vattino 
            LG, MacGregor CP, Chatain CP, Banks E, Olah VJ, Naskar S, Hill S, 
            Liebergall S, Badiani R, Hyde L, Hanley E, Xu Q, Allaway KC, 
            Goldberg EM, Rowan MJM, Nowakowski TJ, Lee S, Favuzzi E, Kaeser PS, 
            Sjulson L, Batista-Brito R, Takesian AE, Ibrahim LA, Iqbal A, Pelkey
            KA, McBain CJ, Dimidschstein J, Fishell G, Wang Y
  TITLE     An enhancer-AAV toolbox to target and manipulate distinct 
            interneuron subtypes.
  JOURNAL   Neuron. 2025 May 21;113(10):1525-1547.e15. doi: 
            10.1016/j.neuron.2025.05.002.
  PUBMED    40403705
REFERENCE   2  (bases 1 to 7269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.05"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1525-1547.e15"
COMMENT     Sequence Label: pAAV_BiLAMP5e3_Gq_dTomato
FEATURES             Location/Qualifiers
     source          1..7269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     intron          942..1074
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             1097..1123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2912..2968
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2981..3001
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3002..3700
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(3132..3150)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3400..3419
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3594..3617
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3755..4343
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3808..3828)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4226..4237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4372..4453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4409..4428)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4463..4482
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4532..4672
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4532..4661
                     /label=AAV2 ITR
     rep_origin      4747..5202
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4834..4853)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5044..5065
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5219..5238)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5338..5360
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5484..5588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5589..5657,5658..6449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5807..5826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6620..7208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7109..7128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcgat aaaggatctt ggccctgtac gcctaagcat
      181 cccttcggtt tggtagcaat ggatgattgt atgcaccatt ctggttcaca tgagttttct
      241 ttctttcttt tgacagcatc tgattatatt agtgtgtatt catcaggaac aaaatggttc
      301 catcattatt caagtgactt ggattcagaa tcactagacc tatcgaatat gcagatgcaa
      361 aggaaagttg gtctattcag cctatcccgc aacaagccaa gaaaaccctg gcaccaatca
      421 ggaacaacaa aaatggaaga aagtatgtgc ttaataatcg gtatcattct ctgaagtaaa
      481 taaaagtgct cagattagtg gcaaagctca ttggttcttt taatatcaat tgtaatgcca
      541 tgaatttttc ccttagctac tgtttcttat tgaaatgaac tgaaataact ctaaaagaaa
      601 catctattat atgactgcaa atgttggagc agatataaat ctgaataggc ttatgagtag
      661 agtttctatc tttgccactg gcttttaatt attttcctaa atcacatata actttgatga
      721 aagtgaatat tgctattttc tgtcagccat ggttgaatga catcaacaca ggagccatta
      781 tcgatgttta aacttcctgc agcccgggct gggcataaaa gtcagggcag agccatctat
      841 tgcttacatt tgcttctagc ctgcaagtcg aggagcgcag ccttccagaa gcagagcgcg
      901 gcgccttaag ctgcagaagt tggtcgtgag gcactgggca cgtaagtatc aaggttacaa
      961 gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt
     1021 tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc acagcgagct
     1081 cgctagcgcc accatgtacc catacgatgt tccagattac gctaccttgc acaataacag
     1141 tacaacctcg cctttgtttc caaacatcag ctcctcctgg atacacagcc cctccgatgc
     1201 agggctgccc ccgggaaccg tcactcattt cggcagctac aatgtttctc gagcagctgg
     1261 caatttctcc tctccagacg gtaccaccga tgaccctctg ggaggtcata ccgtctggca
     1321 agtggtcttc atcgctttct taacgggcat cctggccttg gtgaccatca tcggcaacat
     1381 cctggtaatt gtgtcattta aggtcaacaa gcagctgaag acggtcaaca actacttcct
     1441 cttaagcctg gcctgtgccg atctgattat cggggtcatt tcaatgaatc tgtttacgac
     1501 ctacatcatc atgaatcgat gggccttagg gaacttggcc tgtgacctct ggcttgccat
     1561 tgactgcgta gccagcaatg cctctgttat gaatcttctg gtcatcagct ttgacagata
     1621 cttttccatc acgaggccgc tcacgtaccg agccaaacga acaacaaaga gagccggtgt
     1681 gatgatcggt ctggcttggg tcatctcctt tgtcctttgg gctcctgcca tcttgttctg
     1741 gcaatacttt gttggaaaga gaactgtgcc tccgggagag tgcttcattc agttcctcag
     1801 tgagcccacc attacttttg gcacagccat cgctggtttt tatatgcctg tcaccattat
     1861 gactatttta tactggagga tctataagga aactgaaaag cgtaccaaag agcttgctgg
     1921 cctgcaagcc tctgggacag aggcagagac agaaaacttt gtccacccca cgggcagttc
     1981 tcgaagctgc agcagttacg aacttcaaca gcaaagcatg aaacgctcca acaggaggaa
     2041 gtatggccgc tgccacttct ggttcacaac caagagctgg aaacccagct ccgagcagat
     2101 ggaccaagac cacagcagca gtgacagttg gaacaacaat gatgctgctg cctccctgga
     2161 gaactccgcc tcctccgacg aggaggacat tggctccgag acgagagcca tctactccat
     2221 cgtgctcaag cttccgggtc acagcaccat cctcaactcc accaagttac cctcatcgga
     2281 caacctgcag gtgcctgagg aggagctggg gatggtggac ttggagagga aagccgacaa
     2341 gctgcaggcc cagaagagcg tggacgatgg aggcagtttt ccaaaaagct tctccaagct
     2401 tcccatccag ctagagtcag ccgtggacac agctaagact tctgacgtca actcctcagt
     2461 gggtaagagc acggccactc tacctctgtc cttcaaggaa gccactctgg ccaagaggtt
     2521 tgctctgaag accagaagtc agatcactaa gcggaaaagg atgtccctgg tcaaggagaa
     2581 gaaagcggcc cagaccctca gtgcgatctt gcttgccttc atcatcactt ggaccccata
     2641 caacatcatg gttctggtga acaccttttg tgacagctgc atacccaaaa ccttttggaa
     2701 tctgggctac tggctgtgct acatcaacag caccgtgaac cccgtgtgct atgctctgtg
     2761 caacaaaaca ttcagaacca ctttcaagat gctgctgctg tgccagtgtg acaaaaaaaa
     2821 gaggcgcaag cagcagtacc agcagagaca gtcggtcatt tttcacaagc gcgcacccga
     2881 gcaggccttg tccggagata acggaagcgg agccactaac ttctccctgt tgaaacaagc
     2941 aggggatgtc gaagagaatc ccgggccatc tagaatggcc cccaagaaga agaggaaggt
     3001 ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     3061 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     3121 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     3181 cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     3241 cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3301 ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     3361 caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     3421 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     3481 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     3541 ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga
     3601 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     3661 ccaccacctg ttcctgtacg gcatggacga gctgtacaag taagtcgacg gcgcgcccca
     3721 cccctgcagg gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3781 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3841 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3901 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3961 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4021 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4081 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4141 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     4201 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4261 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4321 ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat caacttgttt
     4381 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4441 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc agcttatcga
     4501 taccgcatgc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     4561 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4621 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     4681 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     4741 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4801 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     4861 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4921 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     4981 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5041 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
     5101 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
     5161 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     5221 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     5281 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5341 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5401 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5461 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5521 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5581 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5641 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5701 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5761 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5821 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5881 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5941 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6001 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6061 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6121 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6181 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6241 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6301 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6361 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6421 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6481 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6541 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7261 ctcacatgt
//
