LOCUS       sequence-421858-        6087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV construct expressing jGCaMP8m driven by Lamp5 
            interneuron-targeting enhancer. Alias: pAAV_WDL0003_jGCaMP8m.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Furlanis E, Dai M, Leyva Garcia B, Tran T, Vergara J, Pereira A, 
            Gorissen BL, Wills S, Vlachos A, Hairston A, Dwivedi D, Du S, 
            McMahon J, Huang S, Morabito A, Vazquez A, Kim S, Lee AT, Chang EF, 
            Razzaq T, Qazi A, Vargish G, Yuan X, Caccavano A, Hunt S, 
            Chittajallu R, McLean N, Hewitt L, Paranzino E, Rice H, Cummins AC, 
            Plotnikova A, Mohanty A, Tangen AC, Shin JH, Azadi R, Eldridge MAG, 
            Alvarez VA, Averbeck BB, Alyahyay M, Vallejo TR, Soheib M, Vattino 
            LG, MacGregor CP, Chatain CP, Banks E, Olah VJ, Naskar S, Hill S, 
            Liebergall S, Badiani R, Hyde L, Hanley E, Xu Q, Allaway KC, 
            Goldberg EM, Rowan MJM, Nowakowski TJ, Lee S, Favuzzi E, Kaeser PS, 
            Sjulson L, Batista-Brito R, Takesian AE, Ibrahim LA, Iqbal A, Pelkey
            KA, McBain CJ, Dimidschstein J, Fishell G, Wang Y
  TITLE     An enhancer-AAV toolbox to target and manipulate distinct 
            interneuron subtypes.
  JOURNAL   Neuron. 2025 May 21;113(10):1525-1547.e15. doi: 
            10.1016/j.neuron.2025.05.002.
  PUBMED    40403705
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.05"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1525-1547.e15"
COMMENT     Sequence Label: pAAV_BiLAMP5e3_jGCaMP8m
FEATURES             Location/Qualifiers
     source          1..6087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     intron          942..1074
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1057..1076
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1155..1172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     1454..1475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1581..1602)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1842..1861)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2433..3021
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2486..2506)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2904..2915)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3190..3271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3227..3246)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3281..3300
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3350..3490
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3350..3479
                     /label=AAV2 ITR
     rep_origin      3565..4020
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3652..3671)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3862..3883
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4037..4056)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4156..4178
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4216..4234)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4302..4406
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4407..4475,4476..5267)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4625..4644)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5438..6026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5927..5946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcgat aaaggatctt ggccctgtac gcctaagcat
      181 cccttcggtt tggtagcaat ggatgattgt atgcaccatt ctggttcaca tgagttttct
      241 ttctttcttt tgacagcatc tgattatatt agtgtgtatt catcaggaac aaaatggttc
      301 catcattatt caagtgactt ggattcagaa tcactagacc tatcgaatat gcagatgcaa
      361 aggaaagttg gtctattcag cctatcccgc aacaagccaa gaaaaccctg gcaccaatca
      421 ggaacaacaa aaatggaaga aagtatgtgc ttaataatcg gtatcattct ctgaagtaaa
      481 taaaagtgct cagattagtg gcaaagctca ttggttcttt taatatcaat tgtaatgcca
      541 tgaatttttc ccttagctac tgtttcttat tgaaatgaac tgaaataact ctaaaagaaa
      601 catctattat atgactgcaa atgttggagc agatataaat ctgaataggc ttatgagtag
      661 agtttctatc tttgccactg gcttttaatt attttcctaa atcacatata actttgatga
      721 aagtgaatat tgctattttc tgtcagccat ggttgaatga catcaacaca ggagccatta
      781 tcgatgttta aacttcctgc agcccgggct gggcataaaa gtcagggcag agccatctat
      841 tgcttacatt tgcttctagc ctgcaagtcg aggagcgcag ccttccagaa gcagagcgcg
      901 gcgccttaag ctgcagaagt tggtcgtgag gcactgggca cgtaagtatc aaggttacaa
      961 gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt
     1021 tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc
     1081 actcccagtt caattacagc tcttaagaaa gctagcgaat tcaagctgct agcaaggatc
     1141 cacccgccac catgcatcat caccatcatc acacgcgtcg caagaagacc ttcaaggagg
     1201 tggccaccgc cgtgaagatc atcggccgcc tgatgggcct gaagatcaac gtctatatca
     1261 aggccgacaa gcagaagaac ggcatcaagg cgaacttcca catccgccac aacatcgagg
     1321 acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc gacggccccg
     1381 tgctgctgcc cgacaaccac tacctgagcg tggagtccaa actttcgaaa gaccccaacg
     1441 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1501 tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc gaggagctgt
     1561 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1621 gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1681 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1741 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1801 tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1861 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1921 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac ctgccggacc
     1981 aactgactga agagcagatc gcagaataca aagaggcttt ctccctattt gacaaggacg
     2041 gggatgggac aataacaacc aaggagctgg ggacggtgat gcggtctctg gggcagaacc
     2101 ccacagaagc agagctgcag gacatgatca atgaagtaga tgccgacggt gacggcacaa
     2161 tcgacttccc tgagttcctg acaatgatgg caagaaaaat gaaatacagg gacacggaag
     2221 aagaaattag agaagcgttc ggtgtgtttg ataaggatgg caatggctac atcagtgcag
     2281 cagagcttcg ccacgtgatg acaaaccttg gagagaagtt aacagatgaa gaggttgatg
     2341 aaatgatcag ggaagcagac atcgatgggg atggtcaggt aaactacgaa gagtttgtac
     2401 aaatgatgac agcgaagtaa aagcttatcg ataatcaacc tctggattac aaaatttgtg
     2461 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2521 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2581 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2641 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2701 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     2761 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     2821 cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg
     2881 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     2941 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     3001 ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct gtgatagcgg
     3061 ccatcaagct ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     3121 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     3181 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3241 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3301 aatgtatcag cttatcgata ccgcatgcac gtgcggaccg agcggccgca ggaaccccta
     3361 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     3421 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     3481 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3541 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3601 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
     3661 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3721 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3781 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3841 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
     3901 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
     3961 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     4021 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     4081 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4141 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4201 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4261 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4321 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4381 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4441 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4501 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4561 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4621 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4681 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4741 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4801 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4861 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4921 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4981 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5041 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5101 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5161 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5221 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5281 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5341 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5581 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5641 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5701 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5761 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5821 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5881 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5941 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6001 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6061 ccttttgctg gccttttgct cacatgt
//
