LOCUS       sequence-421871-        7304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV construct expressing HA-hM3Dq-dTomato driven by PV+ basket 
            cell-targeting enhancer. Alias: pAAV_WDP0003_Gq_dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Furlanis E, Dai M, Leyva Garcia B, Tran T, Vergara J, Pereira A, 
            Gorissen BL, Wills S, Vlachos A, Hairston A, Dwivedi D, Du S, 
            McMahon J, Huang S, Morabito A, Vazquez A, Kim S, Lee AT, Chang EF, 
            Razzaq T, Qazi A, Vargish G, Yuan X, Caccavano A, Hunt S, 
            Chittajallu R, McLean N, Hewitt L, Paranzino E, Rice H, Cummins AC, 
            Plotnikova A, Mohanty A, Tangen AC, Shin JH, Azadi R, Eldridge MAG, 
            Alvarez VA, Averbeck BB, Alyahyay M, Vallejo TR, Soheib M, Vattino 
            LG, MacGregor CP, Chatain CP, Banks E, Olah VJ, Naskar S, Hill S, 
            Liebergall S, Badiani R, Hyde L, Hanley E, Xu Q, Allaway KC, 
            Goldberg EM, Rowan MJM, Nowakowski TJ, Lee S, Favuzzi E, Kaeser PS, 
            Sjulson L, Batista-Brito R, Takesian AE, Ibrahim LA, Iqbal A, Pelkey
            KA, McBain CJ, Dimidschstein J, Fishell G, Wang Y
  TITLE     An enhancer-AAV toolbox to target and manipulate distinct 
            interneuron subtypes.
  JOURNAL   Neuron. 2025 May 21;113(10):1525-1547.e15. doi: 
            10.1016/j.neuron.2025.05.002.
  PUBMED    40403705
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.05"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1525-1547.e15"
COMMENT     Sequence Label: pAAV_BiPVe3_Gq_dTomato
FEATURES             Location/Qualifiers
     source          1..7304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     intron          977..1109
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             1132..1158
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2947..3003
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3016..3036
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3037..3735
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(3167..3185)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3435..3454
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3629..3652
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3790..4378
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3843..3863)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4261..4272)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4407..4488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4444..4463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4498..4517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4567..4707
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4567..4696
                     /label=AAV2 ITR
     rep_origin      4782..5237
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4869..4888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5079..5100
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5254..5273)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5373..5395
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5519..5623
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5624..5692,5693..6484)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5842..5861)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6655..7243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7144..7163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcgat agaccgctgt tgcagtattt cagggaacaa
      181 gatcagaatg gtatagccta cctgaaaaat aggcaatcct ttcaaaatta aatgcacttt
      241 ctcttgggta ggagacaaga taatctatga taagggaaga gagatgtctc cagcttttat
      301 tttttttctt gtaatttgca tttaagccct ggtaacagtt taatatgaag gtgactatgg
      361 cagctccatt tattcaatct atcaatactc tgttgtcaca ggaggagcca tgaggacttg
      421 agtgcagagg aggaatcccc acaaagcagt cttcactctt atcatagctt ccatgtgccc
      481 ttcttattca gcagctctca ttaaaggaac actatcttta gcacagggat tctacttgac
      541 attgacaagg acaggctcag tagcaaatgg gccaaaacta tgaataggcg attcacagca
      601 gagtaaaatc caatggcagc agacataaaa agaagctcaa aatgacaaac aagtcagaca
      661 aacacaaatt caagtaacaa taaaaaatgg agcactggtt tgactcgtac acaagcaaac
      721 agacacaaac aaaacaaaag agtcattaca cctggggcta aagacttctc ttatgtattg
      781 caaagtggaa ataagacctc caggcaaaat gtgaatcgat gtttaaactt cctgcagccc
      841 gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt ctagcctgca
      901 agtcgaggag cgcagccttc cagaagcaga gcgcggcgcc ttaagctgca gaagttggtc
      961 gtgaggcact gggcacgtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
     1021 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
     1081 tgacatccac tttgcctttc tctccacagc gagctcgcta gcgccaccat gtacccatac
     1141 gatgttccag attacgctac cttgcacaat aacagtacaa cctcgccttt gtttccaaac
     1201 atcagctcct cctggataca cagcccctcc gatgcagggc tgcccccggg aaccgtcact
     1261 catttcggca gctacaatgt ttctcgagca gctggcaatt tctcctctcc agacggtacc
     1321 accgatgacc ctctgggagg tcataccgtc tggcaagtgg tcttcatcgc tttcttaacg
     1381 ggcatcctgg ccttggtgac catcatcggc aacatcctgg taattgtgtc atttaaggtc
     1441 aacaagcagc tgaagacggt caacaactac ttcctcttaa gcctggcctg tgccgatctg
     1501 attatcgggg tcatttcaat gaatctgttt acgacctaca tcatcatgaa tcgatgggcc
     1561 ttagggaact tggcctgtga cctctggctt gccattgact gcgtagccag caatgcctct
     1621 gttatgaatc ttctggtcat cagctttgac agatactttt ccatcacgag gccgctcacg
     1681 taccgagcca aacgaacaac aaagagagcc ggtgtgatga tcggtctggc ttgggtcatc
     1741 tcctttgtcc tttgggctcc tgccatcttg ttctggcaat actttgttgg aaagagaact
     1801 gtgcctccgg gagagtgctt cattcagttc ctcagtgagc ccaccattac ttttggcaca
     1861 gccatcgctg gtttttatat gcctgtcacc attatgacta ttttatactg gaggatctat
     1921 aaggaaactg aaaagcgtac caaagagctt gctggcctgc aagcctctgg gacagaggca
     1981 gagacagaaa actttgtcca ccccacgggc agttctcgaa gctgcagcag ttacgaactt
     2041 caacagcaaa gcatgaaacg ctccaacagg aggaagtatg gccgctgcca cttctggttc
     2101 acaaccaaga gctggaaacc cagctccgag cagatggacc aagaccacag cagcagtgac
     2161 agttggaaca acaatgatgc tgctgcctcc ctggagaact ccgcctcctc cgacgaggag
     2221 gacattggct ccgagacgag agccatctac tccatcgtgc tcaagcttcc gggtcacagc
     2281 accatcctca actccaccaa gttaccctca tcggacaacc tgcaggtgcc tgaggaggag
     2341 ctggggatgg tggacttgga gaggaaagcc gacaagctgc aggcccagaa gagcgtggac
     2401 gatggaggca gttttccaaa aagcttctcc aagcttccca tccagctaga gtcagccgtg
     2461 gacacagcta agacttctga cgtcaactcc tcagtgggta agagcacggc cactctacct
     2521 ctgtccttca aggaagccac tctggccaag aggtttgctc tgaagaccag aagtcagatc
     2581 actaagcgga aaaggatgtc cctggtcaag gagaagaaag cggcccagac cctcagtgcg
     2641 atcttgcttg ccttcatcat cacttggacc ccatacaaca tcatggttct ggtgaacacc
     2701 ttttgtgaca gctgcatacc caaaaccttt tggaatctgg gctactggct gtgctacatc
     2761 aacagcaccg tgaaccccgt gtgctatgct ctgtgcaaca aaacattcag aaccactttc
     2821 aagatgctgc tgctgtgcca gtgtgacaaa aaaaagaggc gcaagcagca gtaccagcag
     2881 agacagtcgg tcatttttca caagcgcgca cccgagcagg ccttgtccgg agataacgga
     2941 agcggagcca ctaacttctc cctgttgaaa caagcagggg atgtcgaaga gaatcccggg
     3001 ccatctagaa tggcccccaa gaagaagagg aaggtggtga gcaagggcga ggaggtcatc
     3061 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
     3121 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     3181 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
     3241 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     3301 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     3361 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     3421 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     3481 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     3541 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     3601 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     3661 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg
     3721 gacgagctgt acaagtaagt cgacggcgcg ccccacccct gcagggaatt cgatatcaag
     3781 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3841 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3901 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3961 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4021 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4081 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4141 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4201 tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4261 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4321 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     4381 cgataccgag cgctgctcga gagatcaact tgtttattgc agcttataat ggttacaaat
     4441 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4501 gtttgtccaa actcatcaat gtatcagctt atcgataccg catgcacgtg cggaccgagc
     4561 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     4621 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     4681 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     4741 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     4801 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     4861 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4921 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4981 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5041 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5101 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     5161 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5221 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5281 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5341 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     5401 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     5461 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5521 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5581 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5641 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5701 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5761 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5821 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5881 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5941 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6001 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6061 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6121 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6181 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6241 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6301 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6361 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6421 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6481 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6541 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6601 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6661 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6721 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6781 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6841 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6901 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6961 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7021 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7081 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7141 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7201 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7261 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
